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Klebsiella oxytoca and application thereof

A technology of Klebsiella and seeds, applied in the field of microorganisms, can solve problems such as difficult to solve multiple problems at the same time, achieve the effects of low production cost, increase crop yield, and simple operation

Active Publication Date: 2019-08-20
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the microorganisms reported so far have the functions of dissolving phosphorus, dissolving potassium, nitrogen fixation, and salt tolerance, basically only have one or two of these functions, and it is difficult to solve multiple problems at the same time

Method used

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  • Klebsiella oxytoca and application thereof
  • Klebsiella oxytoca and application thereof
  • Klebsiella oxytoca and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Qualitative detection of phosphorus-solubilizing function of Klebsiella oxytoca YJY19-04

[0036]Using calcium phosphate as the source of inorganic phosphorus, the formula of inorganic phosphorus PVK solid medium is: glucose 10g, ammonium sulfate 0.5g, sodium chloride 0.2g, magnesium sulfate 0.1g, potassium chloride 0.2g, yeast extract 0.5g, manganese sulfate 0.03 g, 0.03g of ferrous sulfate, 0.4% bromophenol blue, 15g of agar, 1000ml of distilled water, pH 7.0-7.2. Pour the melted 1.5% plain agar (sterilized) into the sterilized plate to form a thin layer of agar just enough to cover the bottom of the plate. After the agar has solidified, place three Oxford cups on each plate according to the three corners of an equilateral triangle, pour 10ml of inorganic phosphorus PVK medium into each plate, and take out the Oxford cups with sterilized tweezers after solidification. Add 30ul of bacterial solution to each well and incubate at 30°C for 5-7d. Measure the d...

Embodiment 2

[0042] Example 2: Colonization ability of Klebsiella oxytocia YJY19-04 in soil and its influence on available phosphorus content in soil

[0043] Soil samples were taken from tomato greenhouses in Dianzi Town, Boxing County, Binzhou City, and large pieces of soil were crushed, and soil with smaller particle size was taken for experiments. Pick a single colony of Klebsiella oxytoca and inoculate it in 100ml of LB liquid medium, cultivate overnight at 37°C at 170rpm, centrifuge at 5500rpm, resuspend the bacteria in distilled water for later use. Put the soil in a seedling pot for experiments, and take samples every 7 days to detect changes in the content of available phosphorus in the soil. The specific system configuration is as follows:

[0044] 1#: blank soil;

[0045] 2#: Soil + 1ml YJY19-04 bacterial suspension;

[0046] 3#: Soil + 1% tricalcium phosphate;

[0047] 4#: Soil + 1% tricalcium phosphate + 1ml YJY19-04.

[0048] The available phosphorus in the soil was detec...

Embodiment 3

[0051] Embodiment 3: detection of Klebsiella oxytoca YJY19-04 available phosphorus (inorganic phosphorus)

[0052] Inoculate colony YJY19-04 into LB liquid medium, culture overnight at 35°C 170rpm, adjust OD 600 =1, inoculate in inorganic phosphorus medium according to 1% inoculum size (inorganic phosphorus medium NBRIP (g / L): glucose 10g, calcium phosphate 5g, ammonium sulfate 0.1g, potassium chloride 0.2g, magnesium sulfate 0.25g, Magnesium chloride heptahydrate 5g, distilled water 1L, pH 6.8-7.0, high temperature sterilization at 120°C for 20min), after culturing at 30°C and 200rpm for 72h, the fermentation broth was tested for available phosphorus with reference to the agricultural industry standard NY412-2000. From the detection results, the available phosphorus content in the inorganic phosphorus fermentation broth of Klebsiella oxytoca YJY19-04 is 2.62%, which is 4 times that of the 0.656% available phosphorus content disclosed in paragraph 0032 of the existing patent C...

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Abstract

The invention relates to the technical field of microorganisms, and discloses klebsiella oxytoca and an application thereof. The preservation number of the klebsiella oxytoca is CGMCC No.17360. The invention provides the klebsiella oxytoca which has the effects of dissolving phosphorus, dissolving potassium, resisting salt and the like at the same time. The strain can convert indissolvable phosphorus and potassium elements into rapidly available phosphorus and rapidly available potassium elements of which the state can be directly utilized by plants; and besides, the strain has the effects offixing nitrogen and resisting diseases, the applying quantity of fertilizers is reduced, and besides, the crop yield is increased. The klebsiella oxytoca can be applied to preparation of high-efficiency microbial preparation, has the advantages of being low in production cost, simple to operate, free from secondary pollution to soil and capable of improving crumb structure of soil, can be subjected to field planting in ordinary soil and can be adapted to alkaline soil environment.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a kind of Klebsiella oxytoca and its application. Background technique [0002] Nitrogen, phosphorus, and potassium are the three major nutrients necessary for the growth and development of crops, and play an extremely important role in the growth and development of plants. The soil in our country is rich in phosphorus and potassium elements, but the vast majority of phosphorus and potassium elements exist in the form of insoluble salts, and more than 90% of them cannot be directly absorbed and utilized by plants. How to increase the phosphorus and potassium content in the soil has become an important factor limiting the growth of crops. In order to increase the yield of crops, it is necessary to apply a large amount of chemical fertilizers or organic fertilizers every year, but more than 70% of the applied fertilizers are accumulated in the soil, and the utilization rate of fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C05G3/04C12R1/22C05G3/80
CPCC12N1/20C05G3/00C05G3/80C12R2001/22C12N1/205
Inventor 任晓燕马韵升吴文雷杨传伦张心青李琦杨丹丹倪建龙王玉鑫孙建忠
Owner CHAMBROAD CHEM IND RES INST CO LTD
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