Klebsiella oxytoca strain and application thereof, and triazophos pesticide residue degrading bacterium and preparation method thereof

A Klebsiella oxytoca, pesticide residue degradation technology, applied in chemical instruments and methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve the unreasonable use or even abuse of chemical pesticides, ecosystem hazards, Excessive pesticide residues and other problems, to achieve good pesticide removal effect, promote crop growth, and good removal effect

Active Publication Date: 2012-07-11
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the irrational use or even abuse of chemical pesticides has led to the loss of a large amount of chemical pesticides into the ecological environment, causing serious harm to the ecological system; The pestici

Method used

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  • Klebsiella oxytoca strain and application thereof, and triazophos pesticide residue degrading bacterium and preparation method thereof
  • Klebsiella oxytoca strain and application thereof, and triazophos pesticide residue degrading bacterium and preparation method thereof
  • Klebsiella oxytoca strain and application thereof, and triazophos pesticide residue degrading bacterium and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] a kind of like figure 1 The shown Klebsiella oxytoca strain of the present invention is preserved in China Center for Type Culture Collection CCTCC, and the preservation number is CCTCC M 2011350. This strain has the following main characteristics:

[0035] spherical, capsulated, G - , V-P reaction is positive, methyl red reaction is positive, gelatin liquefaction is negative, indole reaction is positive, the optimum growth temperature is 25℃~35℃, the optimum growth pH value is 5~9, the length of 16S rDNA sequence is 1505bp, in Genbank The accession number in is JQ068819.

[0036] The Klebsiella oxytoca strain of this example can be used to degrade triazophos pesticide residues. For example, a triazophos pesticide residue-degrading bacterial agent comprising the Klebsiella oxytoca strain of the present embodiment of the present invention, the bacterial agent is prepared by the bacterial strain of the present embodiment and the MM medium at a concentration of 2 × 10 ...

Embodiment 2

[0060] Example 2: The bacterial strain degrades triazophos in water samples in farmland drainage ditches.

[0061] Dissolve triazophos with acetone, add in the water sample, be 20mg / L to the final concentration of triazophos, the triazophos pesticide residue degrading bacterial agent prepared in embodiment 1 is inoculated into by 1% (v / v) cultured in this water sample.

[0062] Sampling at regular intervals, extracting triazophos with petroleum ether, spectrophotometer (Tu-1901) measures triazophos residues, and is used to measure standard curve with known triazophos concentration gradient before measurement (see Table 1 and Figure 4 ). The water sample without the addition of bacteria was used as the control. All treatments were repeated three times, and the measurement results are shown in Table 4 below.

[0063] As can be seen from Table 4, the bacterial strain of Example 1 was used to degrade triazophos, and the degradation rate in 20 min was 0.30 mg / L min.

[0064] T...

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Abstract

The invention discloses a Klebsiella oxytoca strain, which is collected in China Center for Type Culture Collection (CCTCC) and has the collection number of CCTCC M2011350. The strain can be applied to degrading triazophos pesticide residue, for example, the strain can be prepared into a triazophos pesticide residue degrading bacterium. The bacterium is bacterium suspension mainly prepared from the strain and a basic liquid nutrient medium; and a preparation method for the bacterium comprises the following steps of: culturing the preserved strain by using a plating method until a single colony appears; inoculating the single colony into a seed culture bottle, and culturing by using a seed medium until an exponential phase; inoculating into a production bottle, and culturing by using a production medium until the exponential phase, wherein the inoculating amount of nutrient solution is not less than 1 percent of the total volume of the production medium for the strain; and taking out of the bottle and filling. The invention has the advantages of low production cost, high pesticide removing effect, no toxin, environment friendliness and the like.

Description

technical field [0001] The invention relates to a bacterium and its application, in particular to a bacterial strain and its application in degrading pesticide residues. Background technique [0002] At present, chemical pesticides have been widely used in modern pesticide production and sanitation and epidemic prevention. The extensive use of chemical pesticides has greatly promoted the development of modern agricultural production. However, the irrational use or even abuse of chemical pesticides has led to the loss of a large amount of chemical pesticides into the ecological environment, causing serious harm to the ecological system; The pesticide residues in agricultural products exceed the standard, which affects the quality of agricultural products, poses a serious threat to people's health, and has a negative impact on the export of agricultural products. [0003] Organophosphorus insecticides are the most widely used and the largest amount of insecticides in my count...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C02F3/34A01N63/02A01P1/00A01P3/00A01P21/00C12R1/22A62D101/04A62D101/28
Inventor 张松柏刘勇张德咏任磊罗香文彭静程菊娥谭新球成飞雪朱春晖杨春晓
Owner HUNAN PLANT PROTECTION INST
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