Method of preparing 2,3-butanediol by simultaneous saccharification and fermentation of corncob residues
A simultaneous saccharification and fermentation, corncob technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high cost, difficult industrial production, cumbersome process, etc., and achieve good efficiency, good saccharification and fermentation, The effect of reducing environmental pollution
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Embodiment 1
[0018] The corncob residue, which is a waste of corn cob production and xylose industry, is crushed and added to 1.5% NaOH solution at a solid-to-liquid ratio of 1:8 (dry weight of cellulose raw material: NaOH solution, W: V), and pretreated at 80°C After 3 hours, the residue was washed until neutral, dried and pulverized. The pretreated residue contained 88.92% cellulose and 3.35% lignin. The content of lignin, which affects the hydrolysis of cellulose, was significantly reduced.
[0019] The treated and untreated corncob residues were added to the fermentation broth containing 1.61g / L urea according to the ratio of cellulose to fermentation broth 1:8 (W / V), the filling volume was 50mL / 250mL, and the initial pH was 5.0. After sterilization, add cellulase at a rate of 400 U per gram of corncob residue, insert 10% Klebsiella pneumoniae (Klebsiella pneumoniae) CICC10011 bacterial liquid, 35 ° C, 150 r / min shake flask fermentation 36h, in the fermentation broth 2 , and the conce...
Embodiment 2
[0022] The corn cob residue, which is a waste of corn cob production xylose industry, is crushed and added to 1.5% NaOH solution at a solid-to-liquid ratio of 1:10 (dry weight of cellulose raw material: NaOH solution, W: V), and pretreated at 80°C After 3 hours, the residue was washed to neutral, dried and pulverized.
[0023] The treated corncob residue is added to the mixture containing urea 1.61g / L, lactic acid 2g / L, sodium citrate 1g / L, EDTA by solid-liquid ratio 1: 10 (dry weight of cellulose raw material: fermented liquid, W: V). .006g / L, dipotassium hydrogen phosphate 5g / L, potassium dihydrogen phosphate 20g / L, magnesium sulfate 0.25g / L, calcium sulfate 0.05g / L, ferrous sulfate 0.15g / L, cobalt chloride 0.005g / L , copper sulfate 0.0075g / L, zinc sulfate 0.15g / L, manganese sulfate 0.05g / L fermentation broth, liquid volume 50mL / 250mL, initial pH 5.0, after sterilization, add in the proportion of 400U per gram of corn cob residue Cellulase, insert 5% Klebsiella pneumoniae (...
Embodiment 3
[0025] The corn cob residue, which is a waste of the corncob production xylose industry, is crushed and added to the 1.5% NaOH solution at a solid-to-liquid ratio of 1:12 (dry weight of cellulose raw material: NaOH solution, W: V), and pretreated at 80°C After 3 hours, the residue was washed to neutral, dried and pulverized.
[0026]The corncob residue after treatment is added to the mixture containing urea 1.61g / L, lactic acid 2g / L, sodium citrate 1g / L, EDTA by solid-liquid ratio 1: 12 (dry weight of cellulose raw material: fermented liquid, W: V). .006g / L, dipotassium hydrogen phosphate 5g / L, potassium dihydrogen phosphate 20g / L, magnesium sulfate 0.25g / L, calcium sulfate 0.05g / L, ferrous sulfate 0.15g / L, cobalt chloride 0.005g / L , copper sulfate 0.0075g / L, zinc sulfate 0.15g / L, manganese sulfate 0.05g / L fermentation broth, liquid volume 50mL / 250mL, initial pH 5.0, after sterilization, add in the proportion of 400U per gram of corn cob residue Cellulase, insert 5% Klebsiell...
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