Method of preparing 2,3-butanediol by simultaneous saccharification and fermentation of corncob residues

A simultaneous saccharification and fermentation, corncob technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of high cost, difficult industrial production, cumbersome process, etc., and achieve good efficiency, good saccharification and fermentation, The effect of reducing environmental pollution

Active Publication Date: 2012-08-22
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

The chemical method for industrial production of 2,3-butanediol is mainly obtained by hydrolysis of four-carbon hydrocarbons produced during petroleum cracking under high tem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The corncob residue, which is a waste of corn cob production and xylose industry, is crushed and added to 1.5% NaOH solution at a solid-to-liquid ratio of 1:8 (dry weight of cellulose raw material: NaOH solution, W: V), and pretreated at 80°C After 3 hours, the residue was washed until neutral, dried and pulverized. The pretreated residue contained 88.92% cellulose and 3.35% lignin. The content of lignin, which affects the hydrolysis of cellulose, was significantly reduced.

[0019] The treated and untreated corncob residues were added to the fermentation broth containing 1.61g / L urea according to the ratio of cellulose to fermentation broth 1:8 (W / V), the filling volume was 50mL / 250mL, and the initial pH was 5.0. After sterilization, add cellulase at a rate of 400 U per gram of corncob residue, insert 10% Klebsiella pneumoniae (Klebsiella pneumoniae) CICC10011 bacterial liquid, 35 ° C, 150 r / min shake flask fermentation 36h, in the fermentation broth 2 , and the conce...

Embodiment 2

[0022] The corn cob residue, which is a waste of corn cob production xylose industry, is crushed and added to 1.5% NaOH solution at a solid-to-liquid ratio of 1:10 (dry weight of cellulose raw material: NaOH solution, W: V), and pretreated at 80°C After 3 hours, the residue was washed to neutral, dried and pulverized.

[0023] The treated corncob residue is added to the mixture containing urea 1.61g / L, lactic acid 2g / L, sodium citrate 1g / L, EDTA by solid-liquid ratio 1: 10 (dry weight of cellulose raw material: fermented liquid, W: V). .006g / L, dipotassium hydrogen phosphate 5g / L, potassium dihydrogen phosphate 20g / L, magnesium sulfate 0.25g / L, calcium sulfate 0.05g / L, ferrous sulfate 0.15g / L, cobalt chloride 0.005g / L , copper sulfate 0.0075g / L, zinc sulfate 0.15g / L, manganese sulfate 0.05g / L fermentation broth, liquid volume 50mL / 250mL, initial pH 5.0, after sterilization, add in the proportion of 400U per gram of corn cob residue Cellulase, insert 5% Klebsiella pneumoniae (...

Embodiment 3

[0025] The corn cob residue, which is a waste of the corncob production xylose industry, is crushed and added to the 1.5% NaOH solution at a solid-to-liquid ratio of 1:12 (dry weight of cellulose raw material: NaOH solution, W: V), and pretreated at 80°C After 3 hours, the residue was washed to neutral, dried and pulverized.

[0026]The corncob residue after treatment is added to the mixture containing urea 1.61g / L, lactic acid 2g / L, sodium citrate 1g / L, EDTA by solid-liquid ratio 1: 12 (dry weight of cellulose raw material: fermented liquid, W: V). .006g / L, dipotassium hydrogen phosphate 5g / L, potassium dihydrogen phosphate 20g / L, magnesium sulfate 0.25g / L, calcium sulfate 0.05g / L, ferrous sulfate 0.15g / L, cobalt chloride 0.005g / L , copper sulfate 0.0075g / L, zinc sulfate 0.15g / L, manganese sulfate 0.05g / L fermentation broth, liquid volume 50mL / 250mL, initial pH 5.0, after sterilization, add in the proportion of 400U per gram of corn cob residue Cellulase, insert 5% Klebsiell...

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Abstract

The invention discloses a method of preparing 2,3-butanediol by simultaneous saccharification and fermentation of corncob residues with xylose extracted from corncobs and belongs to the technical field of biochemical engineering. The method includes the steps of using the corncob residues as a carbon source and carbamide as a nitrogen source, using klebsiella pneumoniae CICC 10011, klebsiella oxytoca CICC 21518, bacillus polymyxa CICC 10010, bacillus cloacae CICC 10014 and a mixed bacterium solution to perform the simultaneous saccharification and fermentation, and enabling the weight of the 2,3-butanediol and acetoin to reach 19.59%-36.13% of the dry weight of the corncob residues. According to the method of preparing 2,3-butanediol by the simultaneous saccharification and fermentation of the corncob residues, the corncob residues are used as the carbon source, so that production cost is effectively reduced, high value utilization of resources is achieved, and huge economic benefits and social benefits are achieved.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a method for fermenting and producing 2,3-butanediol. Background technique [0002] 2,3-butanediol (2,3-butanediol, referred to as 2,3-BD) is widely used in many fields such as chemical industry, food and aerospace. Its calorific value is 27200kJ / kg, which is similar to ethanol (29100kJ / kg). It can be used as a fuel. At the same time, it can also be used to prepare polymers, inks, perfumes, antifreezes, aromatherapy agents, humidifiers, explosives and pharmaceuticals. sex carrier, etc. [0003] With the depletion of petrochemical resources and the impact of the petrochemical industry on the environment, renewable resources have attracted more and more attention. Due to the rising and unstable oil prices, the production of bio-based bulk chemicals such as biofuels from renewable resources has attracted great interest. Using renewable biomass resources as substrates a...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12P39/00C12R1/22C12R1/12C12R1/07
Inventor 肖冬光张翠英郭学武陈叶福杜丽平董健彭晓培
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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