41results about How to "Effective enzymatic hydrolysis" patented technology

The invention discloses a polypeptide germinated-soybean milk production method, which comprises the following processes of: selecting soybean; performing germination treatment; grinding into milk; performing heat treatment; performing enzymolysis twice by using mixed enzyme; blending nutrient; homogenizing and sterilizing; performing vacuum deodorization; and performing sterile filling to obtain the finished product. By adopting the mixed enzyme technology, soybean protein is hydrolyzed into polypeptide substances, and substances such as cellulose, pectin and the like are effectively subjected to enzymolysis, so that nutrients are easy to absorb. Nutrition enhancement is carried out aiming at different people, so that the nutritional value of the product is improved and the product has unique physiological functions of preventing and treating cardiovascular disease and the like. Beany flavor and bitter substances of the produced product are removed, so that the product is fine and smooth, and pure in taste, and a process technology route is reasonable.

The invention belongs to the technical field of preparation of veterinary Chinese medicinal oral liquid, and in particular relates to a method for preparing veterinary heat-clearing and detoxifying oral liquid. The method comprises the steps of crushing of raw medicinal materials, screening, enzymolysis, decocting, micro-filtration, ultra-filtration, reduced pressure concentration, volume setting and the like. Compared with the prior art, the method has the advantages that plant cell walls can be hydrolyzed with an enzyme more effectively, so that the dissolution rate of active ingredients of the medicinal materials is improved; and by combining the micro-filtration and ultra-filtration technologies, impurities are effectively removed from the medicinal liquid, the clarity of the solution is higher, the loss of the active ingredients of the medicinal materials is less, and the clinical treatment effect of the medicaments is well guaranteed. By reasonably matching the bio-enzyme and using the bio-enzyme to assist in extracting the active ingredients in the medicinal materials, the content of key effect ingredients in the medicinal liquid is effectively improved, so that the quality of the product is well improved, and the oral liquid has good popularization and application values.

The invention provides a method for extracting oligopeptide from algae to prepare alga oligopeptide powder. The method includes steps: cleaning, airing and grinding algae to obtain alga powder; adding water for pulp grinding; heating alga pulp, adjusting pH to 3.3-4.0, adding pectinase, and stirring for enzymolysis; adding NaOH, adjusting pH to 7.0-9.0, adding compound protease, and stirring for enzymolysis; heating for enzyme deactivation, and performing solid-liquid separation and liquid phase collection; treating clear liquid according to a membrane filtration method, and collecting permeation liquid; subjecting the permeation liquid to electrodialysis, activated carbon refining, decarburization, evaporative concentration and drying to obtain the alga oligopeptide powder. The method is simple in process and reasonable in design, and small peptides are extracted from algae by enzymolysis to provide products beneficial to human health; high-value utilization of algae is realized, alga utilization rate is increased to make full use of natural resources, and influences of sea water quality and neritic organisms due to large-scale growth of algae are reduced.

The invention belongs to the fields of food biotechnologies and function healthcare and discloses an antihypertensive peptide derived from a sardine as well as a preparation method and application of the antihypertensive peptide. The method comprises the following main technical steps of after pretreating the sardine to obtain homogenate, adding papain to carry out enzymolysis firstly, and then, adding an alkaline protease to carry out enzymolysis; deactivating the enzyme after enzymolysis is finished, and centrifuging the enzyme-deactivated enzymatic hydrolysate; taking the centrifuged supernatant, and separating and purifying a crude product by using an ultrafiltration and gel chromatography method; carrying out freeze-drying on the separated and purified efficient component to obtain the antihypertensive peptide derived from the sardine. The sardine is high in yield and low in price, so that the comprehensive utilization value of the sardine can be sufficiently increased when the sardine is used as the raw material to prepare the antihypertensive peptide; in addition, the antihypertensive peptide derived from the sardine and obtained through enzymolysis is free of safety problems and side effects of chemical antihypertensive drugs and ideal in antihypertensive effect.

The invention discloses carp feed which comprises soybean meal, yellow soybean meal, black soybean powder, wheat flour, millet flour, glutinous millet flour, polished glutinous rice flour, corn flour, rice bran powder, oat powder, sesame powder, linseed powder, egg shells, crab shells, chicken breast, sunflower seeds, rapeseed, fish oil, spirulina, seaweeds, porphyra tenera, carrots, composite trace elements, citric acid, lactic acid, oyster powder, fish meal, glutathione, phospholipid, vitamin C, vitamin E, vitamin A, calcium gluconate, zinc gluconate, acetic acid, DHA (docosahexaenoic acid), plant enzyme and algin. The fish feed disclosed by the invention is rich in nutrition and enriched in various amino acids, proteins and microelements, meanwhile, a variety of organic acids and enzymes are enriched, so that the survival rate of fishes is improved, and the meat quality of fishes can be effectively improved.

Catfish feed is disclosed. The feed includes soybean powder, soybean powder, black bean powder, wheat flour, millet powder, broomcorn millet flour, flour of polished glutinous rice, corn flour, rice bran powder, oat meal, sesame powder, linseed meal, eggshell, crab shells, chicken breast, sunflower seed, rapeseed, fish oil, carrots, compound trace elements, citric acid, lactic acid, oyster powder, fish meal, glutathione, phospholipid, vitamin C, vitamin E, vitamin A, calcium gluconate, zinc gluconate, acetic acid, DHA, plant enzymes, and algin. The feed is rich in nutrition, rich in a plurality of amino acids, proteins and trace elements, and rich in a plurality of organic acids and enzymes, increases the survival rate of fishes and effectively improves meat quality of fishes.

The invention discloses milk cow feed which comprises silage, wheat barn, corns, carrots, pumpkins, magnesium sulfate, calcium gluconate, zinc gluconate, fish oil, spirit lees, citric acid, table salt, soy meal, soybean meal, black soybean meal, wheat meal, millet flour, yellow rice flour, polished glutinous rice flour, rice bran powder, sesame powder, linseed meal, egg shells, crab shells, chicken breasts, sunflower seeds, rapeseeds, spirulina, seaweeds, lavers, compound trace elements, lactic acid, oyster shell power, fish meal, glutathione, phospholipid, vitamins C, vitamins E, vitamins A, acetic acid, DHA (Docosahexaenoic Acid), plant enzymes, algin, bavin bugs, omphisa fuscidentalis hampson, tenebrio molitor, scoop rice worms, silkworm chrysalis, bean worms and cicada. The milk cow feed disclosed by the invention is rich in nutrition, rich in a variety of amino acids, proteins and trace elements and meanwhile rich in a variety of organic acids and enzymes, so that not only can the survival rate of milk cows be improved, but also the quality of the milk cows can be effectively improved.

The invention discloses a crab shell swing-type enzymolysis device, which is characterized by comprising: an enzymolysis pool (1), a crab cage (2), hoisting devices (3) and a cleaning pool (4); two hoisting devices (3) are provided and are symmetrically arranged at two sides of the enzymolysis pool (1) and the cleaning pool (4); the hoisting device (3) comprises a support bar (5), a slide cylinder (6), and lifting rods (7); the lifting rods (7) are located at two ends of the support bar (5); the slide cylinder (6) is located on the support bar (5) and can slide on the support bar (5); a hanging ring of the crab cage (2) at the same side as the slide cylinder (6) is hung below the slide cylinder (6); the enzymolysis pool (1) and the cleaning pool (4) which are parallelly arranged are disposed below the crab cage (2). The crab shell swing-type enzymolysis device provided by the invention enables the crab cage to be in a swing-type motion state driven by the hoisting device, and thus has good dissolution effect on residual crab meat and mucous membranes, and short dissolution time.

The invention relates to an astaxanthin-containing piglet feed additive as well as a preparation method and application thereof and belongs to the field of feed. The preparation method of the astaxanthin-containing piglet feed additive comprises the following steps: carrying out enzymolysis on algae powder with a compound enzyme, so that enzymatic hydrolysate is obtained, and then drying, whereinthe compound enzyme comprises cellulase and protease. The preparation method of the astaxanthin-containing piglet feed additive is simple in operation, can effectively release astaxanthin contained inthe algae powder and is beneficial to improvement of utilization rate of the astaxanthin. The piglet feed additive prepared by adopting the preparation method contains rich astaxanthin and proteins and can effectively improve appetite of piglets and skin color and hair quality of the piglets. The piglet feed additive provided by the invention can obviously improve daily feed intake and daily gainof the piglets and also can effectively improve the skin color and hair quality of the piglets when being used for preparing piglet feed.

The present invention is the preparation process of natural active protein and peptide. The natural material with rich protein is processed through defatting, cleaning, homogenizing, mixing with deionized water inside a bioreactor, pH regulating to 5-8, heating to 70-90 deg.c, maintaining and stirring for 10-30 min, quick cooling to 30-50 deg.c and mixing with composite proteinase for enzymolysis for 2-6 hr. During enzymolysis, the reactant is sampled once every 0.5 hr, and the molecular weight distribution of the product is determined in SDS-PAGE or HPLC / MS method. According to the requirement on the molecular weight of target product, the enzymolysis degree is judged, the end time of enzymolysis is determined, enzyme is deactivated immediately after finishing the reaction and the product is filtered and frozen for preservation.

The invention provides a method for preparing a heavy metal adsorbing material by modifying silk fiber and application of the material and belongs to the technical field of adsorbing materials. The method disclosed by the invention comprises the following steps: (1) pretreatment: taking raw silk of silkworms in the current year, putting the raw silk into a weak alkaline solution, adding lipase, alkaline protease and a surface active agent, utilizing ultrasonic wave to treat, soak and boil the raw silk and then utilizing water to washing the raw silk; (2) constant weight: putting the cleaned silk into a freezing and drying box to be dried, then weighing the dried silk, then freezing and drying the silk and then weighing the silk; (3) detection: sampling the constant-weight silk and utilizing a picric acid carmine reagent to detect the silk; (4) modification: directly soaking fibroin in a neutral protease solution, performing ultrasonic wave treatment and then freezing and drying to constant weight to obtain the needed adsorbing material. The preparation method of the novel heavy-metal adsorbing material disclosed by the invention is low in cost, high in efficiency, simple in technology, convenient to operate and convenient for large-scale application.

The invention belongs to the field of food biotechnology and functional health care, and discloses a sardine hypotensive peptide, a preparation method and application thereof. The main technical steps of the method are as follows: after the sardines are pretreated into a homogenate, first add papain for enzymolysis, then add alkaline protease for enzymolysis; Centrifuging; taking the centrifuged supernatant, using ultrafiltration and gel chromatography to separate and purify the crude product; freeze-drying the separated and purified high-efficiency components to obtain the sardine hypotensive peptide. The output of sardines is high and the price is low. Therefore, using sardines as raw materials to prepare hypotensive peptides can fully improve the comprehensive utilization value of sardines, and the sardine hypotensive peptides obtained by enzymatic hydrolysis have no safety problems and no chemical antihypertensive drugs. Side effects, antihypertensive effect ideal.

The invention relates to the field of plant transgenic technology, in particular to a method for improving the agrobacterium-mediated genetic transformation efficiency of maize. Transgenic plants areobtained by the steps of ultrasonic treatment of seeds to obtain germinated seeds, culture of agrobacteria containing target genes, seed infection and co-culture, agrobacterium removal, selective culture, and molecular detection of transgenic plants. The method can effectively shorten the time of transgene transformation and improve the agrobacterium-mediated genetic transformation efficiency of maize.

The present invention discloses a method for preparing cattle feed from siraitia grosvenorii residues. The preparing method comprises the following steps: adding the siraitia grosvenorii residues to water and carrying out ultrasonic water bath, thereby obtaining a mixed solvent A; adjusting the pH value to 4.5, adding lignocellulose and pectinase enzyme, carrying out enzymolysis for 8min, adjusting the pH value to 7, adding the siraitia grosvenorii protease, carrying out enzymolysis and inactivation; adding salt, honey, dry seaweed, straw, soybean meal, distilled grains, chicken meat, chicken bone meal, aloe, lotus root, andrographis paniculata and EM bacteria; carrying out aerobic fermentation; adding yeast, and carrying out anaerobic fermentation; carrying out drying; adding rapeseeds, sunflower seeds and forsythia suspensa to a wok, and frying the mixture for 3min; adding ginger powder, pepper powder, star aniseed powder, sesame, walnut kernels and poria cocos, frying the mixture for 6min; carrying out stirring and irradiating the mixture by an electron beam for 2min, thereby finally obtaining the cattle feed. The preparation method is simple and efficient; and the prepared cattle feed has a high nutritional value.

The invention discloses a preparation method of sugarcane dietary fibers. The preparation method comprises the following steps of pretreatment, preparation of a semi-finished product, purification andpost-treatment. The invention also discloses the sugarcane dietary fibers prepared by the preparation method of the sugarcane dietary fibers. According to the method provided by the invention, duringpretreatment, a mode with combination of high-temperature cooking treatment, steam explosion treatment and ultrasonic treatment on raw materials is adopted, structural organizations of the raw materials can be effectively destroyed, and inclusions of the raw materials are fully exposed; by combining with impurity removal treatment, non-dietary fiber components in the raw materials are further lowered, and the raw materials after pretreatment are beneficial to strengthening the enzymolysis effect of cellulase or a compound enzyme of the cellulase and hemicellulase and also enables enzymolysisto be more full, so that the purity of a finished product is improved, and the palatability of the finished product can be effectively improved; and the finished product is purified after enzymolysis,the purity is further improved, and the finished product has the advantage of high purity. The sugarcane dietary fibers provided by the invention also have the advantages of small fiber particle sizes, strong water-holding capacity and strong swelling capacity.

The invention relates to a method for producing ethanol by the wood fiber raw material, and solves the problem of low total sugar yield in the prior art. The method for producing ethanol by the wood fiber raw material comprises the following steps of: 1) smashing the wood fiber raw material, effectively and continuously dipping in dilute acid at the temperature of 20-80DEG C, staying for 1-15 minutes, and dewatering; 2) processing the dewatered material under the condition that the temperature is 80-200DEG C and the pressure is 0.4-10MPa for 1-35 minutes; 3) adding water into the material obtained in step 2), blending into required dry matter density, and hydrolyzing by the cellulose for 8-120 hours to obtain enzymatic hydrolysate under the condition that the temperature is 30-70DEG C and pH (potential of hydrogen) is 1-7; 4) carrying out zymophyte co-fermentation for 8-120h under the anaerobic condition that the temperature is 20-45DEG C and the pH is 3-8 to obtain fermentation mash containing ethanol; and 5) after the fermentation mash is subjected to concentration extraction, obtaining the ethanol of which the purity is 95%. According to the technical scheme, the problem is better solved, and the method for producing ethanol by the wood fiber raw material can be used for the industrial production for producing the ethanol by the wood fiber raw material.

The invention relates to novel rhizoma polygonati wine and a production process thereof. The production process comprises the following steps: performing enzymolysis on traditional Chinese medicinal materials, performing diastatic fermentation, performing base liquor fermentation, adding edible alcohol for soaking, filtering, separating and the like. The wine provided by the invention takes the pure traditional Chinese medicinal materials with homology of medicine and food as raw materials and is safe and harmless to the human body; the wine meets the compatibility of the traditional Chinese medicinal materials, and has the effects of strengthening the spleen and stomach, reinforcing liver and kidney, nourishing yin and blood, regulating deficiency, calming the heart and tranquilizing themind and the like; by virtue of steps of filtration, beta-cyclodextrin debitterization and the like, bitterness is removed, and the taste is optimized. The innovation point is that by utilizing a manner of directly fermenting after performing enzymolysis on the traditional Chinese medicinal materials, the active ingredients in the medicinal materials are efficiently and fully released. Compared with the traditional health medicinal liquor, the novel rhizoma polygonati wine disclosed by the invention has the advantages that the production cycle is greatly shortened, the ingredients of the traditional Chinese medicinal materials are fully utilized, and the losses of nutritional ingredients are greatly reduced.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to rose stock solution essence and a preparation method thereof. The rose petals and the rose branches and leaves are subjected to extraction respectively to serve as main raw materials to prepare the essence, and the essence has low content of preservatives, emulsifiers and the like and low irritation to skin, and has calming, relieving, skin softening and whitening effects.

The invention relates to a device and method for extracting xylitol and chlorophyll dietary fiber from whole-plant corn straws. The device comprises a whole-plant corn straw pretreatment system, an enzyme preparation system, an enzymolysis system, a xylitol extraction system and a chlorophyll dietary fiber manufacturing system, wherein the enzymolysis system comprises a hydrolysis kettle and a squeezing-extracting hopper, a solid material inlet and a liquid material inlet are formed in the top of the hydrolysis kettle, the solid material inlet is connected with the whole-plant corn straw pretreatment system, the liquid material inlet is connected with the enzyme preparation system, an outlet is formed in the bottom of the hydrolysis kettle and is connected with the squeezing-extracting hopper, a solid material outlet of the squeezing-extracting hopper is connected with the chlorophyll dietary fiber manufacturing system by virtue of a conveyer, and pipelines for collecting percolate are arranged at outlets of the squeezing-extracting hopper and the hydrolysis kettle and are connected with the xylitol extraction system. The device is reasonable in structure, effective enzymolysis of the whole-plant corn straws can be realized, and the product yield is improved.

The invention discloses livestock raising feed. The feed is prepared from silage, wheat bran, corn, carrots, pumpkin, magnesium sulfate, calcium gluconate, zinc gluconate, fish oil, distiller grains, citric acid, edible salt, soybean meal, black bean meal, wheat flour, millet flour, glutinous millet powder, glutinous rice meal, rice bran meal, sesame powder, linseed meal, egg shells, crab shells, chicken breast, sunflower seeds, rapeseeds, spirulina, seaweed, porphyratrace element complex, lactic acid, oyster powder, fish powder, glutathione, phospholipid, vitamin C, vitamin E, vitamin A, acetic acid, DHA, plant-esterase, peppermint, Chinese dates, astragalus membranaceus, codonopsis pilosula, pericarpium citri reticulatae, ricepaper pith, caulis akebiae, dandelion, cordate houttuynia, as arum, ryegrass, poria cocos, platycodon grandiflorum and lonicera japonica. The livestock raising feed is rich in nutrition, is rich in multiple amino acids, protein and trace elements, and is rich in multiple organic acids and enzyme, so that the survival rate of cattle and sheep can be improved, and the meat quality of cattle and sheep can be effectively improved.

The invention discloses a crab shell enzymolysis device, which is characterized by comprising: an enzymolysis pool (1), a crab cage (2), a support bar (3), a slide cylinder (4), lifting bars (5), and a cleaning pool (6); the slide cylinder (4) is disposed on the support bar (3) and can slide on the support bar (3); the lifting bars (5) are disposed at two ends of the support bar (3); the crab cage (2) is hung below the slide cylinder (4); the enzymolysis pool (1) and the cleaning pool (6) which are parallelly arranged are disposed below the crab cage (2). The crab shell enzymolysis device provided by the invention removes mucous membranes and residual crab meat on crab shell surfaces in an enzymolysis mode. When the enzymolysis completes, the mucous membranes and residual crab meat are enzymolyzed and dissolved out effectively.

The invention provides a crayfish culture method, which belongs to the field of aquaculture, including pretreatment of culture ponds, cultivation of hydroponic algae, seedling feeding and management, feed feeding, and water body purification, wherein the purifying agent used for water body purification includes EM biological bacteria agent and Composite flocculant, the above-mentioned composite flocculant is a polymer formed by β-cyclodextrin as a matrix, and a cross-linking agent is catalyzed to undergo esterification and cross-linking reaction, and the cross-linking agent used includes citric acid, hydroxybenzoic acid and 2- Hydroxypropionic acid. The method for cultivating crayfish provided by the invention has a benign breeding ecological environment, low cultivation cost, good water quality purification, high survival rate and output of crayfish; the feed used in the method is excellent in edibility and palatability, and the production rate and product purity are excellent. High; the specific surface area of ​​the composite flocculant used is relatively large, the cavity structure capacity and adsorption capacity are high, the expansion coefficient is low, the water area is wide, the water purification effect is excellent, the breeding environment is improved, and the survival rate is conducive to improving the economic benefits of breeding.

The invention discloses a brewing method of cherry fruit wine and belongs to the technical field of fruit wine brewing. The method includes: picking fruits and removing impurities, performing secondary cleaning, performing quick freezing, thawing, loading into a tank for impregnation, performing enzymolysis, clarifying, fermenting, pouring into a jar, storing, aging, fining, filtering, performinganti-oxidation treatment, blending, performing freezing treatment, performing sterilizing plate filtration, performing stability tests, precisely filtering, performing quality inspection and filling.The cherry fruit wine brewed by the method is good in taste, stable in quality and the like. The brewing method is capable of shortening the process, reducing energy consumption and lowering production cost.

The invention belongs to the technical field of biology, and particularly relates to an efficient straw enzymolysis method. The efficient straw enzymolysis method comprises the following steps of (1)pretreatment of straws, namely, crushing and sieving the straws, putting the straws into a steamer, steaming with water vapor, adding a mixed solution of potassium hydrogen persulfate and boric acid,keeping warm, standing, adding into deionized water, keeping warm, standing, drying in a drying oven until the weight is constant, and performing high-pressure steam sterilization treatment to obtainpretreated straws; and (2) composite enzymolysis culture, namely, putting the pretreated straws into a culture medium containing trichoderma reesei and rhizopus oryzae, and performing culturing to obtain the product. According to the efficient straw enzymolysis method, the problem that the traditional single cellulase is low in enzymolysis effect is solved, the accessibility is further improved through a pretreatment method, the straw is fully utilized, the enzyme activity is enhanced through a culture medium containing hydrolyzed fish protein powder and cottonseed protein, and therefore, themethod has relatively high economic value.

The present invention belongs to the technical field of veterinary herbal medicines, and discloses a traditional Chinese medicine composition for preventing and treating chicken colibacillosis and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 50-70 parts of herba rhodiolae, 40-60 parts of fructus ligustrilucidi, 20-40 parts of herba centellae, 20-30 parts of herba andrographitis, 20-40 parts of radix berberidis, 20-40 parts of mahonia bealei, 20-40 parts of herba euphorbiae humifusae, 20-30 parts ofcacumen biotae, 20-30 parts of fructus citrus sarcodactylis and 5-15 parts of radix glycyrrhizae. According to parts by weight with the unit of g and parts by volume with the unit of mL, all the raw materials are mixed and ground in proportion, then extraction, impurity removal and concentration are performed, the volume is made up to 1,000 parts by volume, and an oral solution is prepared. The traditional Chinese medicine composition is a pure traditional Chinese medicine preparation, has safety, no toxic or side effects and no palatability problem, can increase the colibacillosis survival rate to 65% or above and can improve the weight gain.

The invention belongs to the technical field of traditional Chinese veterinary medicine, and discloses a traditional Chinese medicine composition for preventing and treating chicken colibacillosis and a preparation method thereof. The traditional Chinese medicine composition is made of the following raw materials in parts by weight: 50-70 parts of Rhodiola rosea, 40-60 parts of Ligustrum lucidum, 20-40 parts of Centella asiatica, 20-30 parts of Andrographis paniculata, 20-30 parts of three needles 40 parts, 20~40 parts of top ten contributions, 20~40 parts of Dijincao, 20~30 parts of Arborvitae leaves, 20~30 parts of bergamot, 5~15 parts of licorice. The parts by weight are in g, and the parts by volume are in mL. The raw materials are mixed according to the proportion, pulverized, then extracted, impurity-removed, concentrated, and the volume is set to 1000 parts by volume to obtain an oral liquid. The invention is a pure traditional Chinese medicine preparation, which is safe, has no toxic and side effects, and has no palatability problem, can increase the survival rate of colibacillosis to more than 65%, and can improve weight gain.

The invention relates to the field of food additives, and particularly discloses a food additive for egg yolk products as well as a preparation method and application of the food additive. The invention discloses a food additive for egg yolk products. The food additive is prepared from the following raw materials in parts by weight: 2-20 parts of sodium alginate / chitosan immobilized phospholipase A, 1-5 parts of a calcium ion adjuvant, 8-16 parts of tea polyphenol, 15-25 parts of astaxanthin, 5-15 parts of alliin and 20-40 parts of water, wherein the concentration of calcium ions in the calcium ion auxiliary agent is 3-11 wt%; according to the food additive prepared by the application, the emulsifying stability of the egg yolk product can be improved, and meanwhile, the microbiological indexes of the egg yolk product are effectively reduced in combination with substances such as tea polyphenol, so that the contradiction between the microbiological indexes and sensory indexes in the egg yolk product is solved; the egg yolk product can be endowed with certain nutritional value, and the health-care effect is improved.

The present invention is the preparation process of natural active protein and peptide. The natural material with rich protein is processed through defatting, cleaning, homogenizing, mixing with deionized water inside a bioreactor, pH regulating to 5-8, heating to 70-90 deg.c, maintaining and stirring for 10-30 min, quick cooling to 30-50 deg.c and mixing with composite proteinase for enzymolysis for 2-6 hr. During enzymolysis, the reactant is sampled once every 0.5 hr, and the molecular weight distribution of the product is determined in SDS-PAGE or HPLC / MS method. According to the requirement on the molecular weight of target product, the enzymolysis degree is judged, the end time of enzymolysis is determined, enzyme is deactivated immediately after finishing the reaction and the product is filtered and frozen for preservation.

The invention provides a method for breeding crayfish, and belongs to the field of aquaculture. The method includes pretreatment of an aquaculture pond, cultivation of algae, seedling and management,fodder feeding and purification of water body; wherein a purifying agent used for purifying the water body comprises an EM biological bacterium agent and a composite flocculating agent; the compositeflocculating agent is a polymer formed by using beta-cyclodextrin as a matrix to carry out a cross-linking reaction with a crosslinking agent; the crosslinking agent comprises citric acid, hydroxybenzoic acid and 2-hydroxypropionic acid. The method for breeding the crayfish provided by the invention has the advantages of good breeding ecological environment, low breeding cost, good water purification, high crayfish survival rate and high yield; the edibility and palatability of the fodder used in the method is excellent, and the preparation yield and the product purity are high; and the used composite flocculating agent has a large specific surface area, high cavity structure capacity and adsorption capacity, low expansion coefficient, wide water area, excellent water purification effect,can improve the breeding environment, can improve the survival rate, and can increase the economic benefits of aquaculture.

The invention belongs to the technical field of cosmetic preparation, and particularly relates to face cream containing a rose stock solution and a preparation method of the face cream. The rose stocksolution and a salicornia herbacea extract are added into the face cream, so that the face cream has the effects of calming, moisturizing and whitening, the extraction rates and the purities of the rose stock solution and the salicornia herbacea are effectively improved, the extraction rates reach 67.5% and 47.8% respectively, and the purities reach 87.1% and 83.9% respectively.