Prepn of natural active protein and peptide

A protein and natural technology, applied in the field of preparation of natural active proteins and peptides, can solve the problems of long time required, complex process, amino acid decomposition, etc., and achieve good enzymatic hydrolysis, efficient degradation, and short heating time

Inactive Publication Date: 2005-11-09
谢君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] (1) In terms of structure and function, natural biologically active proteins and peptides are the most complex compounds in nature, so it is not practical to isolate a certain type of natural protein or peptide from natural raw materials;
[0004] (2) The process is complicated, the reagents and conditions used are relatively harsh, and the unique structure of the pure natural protein must be maintained in order to retain its unique function and biological activity;
[0005] (3) Materials containing protein and peptide products are unstable, and protein products are susceptible to protease degradation, which increases many unstable factors
When the general protease method prepares natural proteins and peptides, the efficiency of hydrolyzing protein raw materials is low, and it takes a long time; while the conventional acid hydrolysis and alkaline hydrolysis methods for preparing natural proteins and peptides, although the efficiency of hydrolyzing protein raw materials is high, However, its inherent three-dimensional structure and conformation have been severely damaged to a large extent, and some amino acids may be decomposed, thereby making its products lose their biological activity; in addition, the acid hydrolysis and alkali hydrolysis processes will also bring environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) 3% Na for animal skin 2 CO 3 The solution was degreased and rinsed 3 times with deionized water;

[0034] (2) After 100 kilograms of pretreated animal hides are homogenized, mix with 150 kilograms of deionized water, and join in the biochemical reactor;

[0035] (3) Adjust the pH to 7, keep stirring at a temperature of 85°C for 10 minutes, and rapidly cool down to 40°C;

[0036] (4) Add 20 grams of multiple compound proteases (mixed by microbial protease, papain, bromelain, pepsin and trypsin in a ratio of approximately 6:1:1:1:1) in the biochemical reactor, after stirring, Enzyme hydrolysis reaction 2 to 6 hours;

[0037] (5) Take a sample every 0.5 hours during the enzymolysis process, and use SDS-PAGE or HPLC / MS method to measure the molecular weight distribution of the biochemical reaction products, and determine the end time of the enzymolysis reaction according to the requirements for the molecular weight of the target product;

[0038] (6) The enzyme is i...

Embodiment 2

[0040] (1) 100 kg of egg white and 100 kg of deionized water are fully mixed and then added to the biochemical reactor;

[0041] (2) Adjust the pH=7 and heat up to 40°C;

[0042] (3) Add 20 grams of multiple compound protease (mixed by microbial protease, papain, bromelain, pepsin and trypsin in a ratio of 6:1:1:1:1) in the biochemical reactor, after stirring , enzymolysis reaction for 2 to 6 hours;

[0043] (4) Take a sample every 0.5 hours during the enzymolysis process, and use SDS-PAGE or HPLC / MS method to measure the molecular weight distribution of the biochemical reaction products, and determine the end time of the enzymolysis reaction according to the requirements for the molecular weight of the target product;

[0044] (5) The enzyme is inactivated, and the reaction product is filtered and frozen.

Embodiment 3

[0046] (1) Cleaning, squeezing, degreasing and other pretreatments of soybeans or corn that have passed strict inspection;

[0047] (2) 100 kg of pretreated soybeans or corn are homogenized, mixed with 100 kg of deionized water, and added to the biochemical reactor;

[0048] (3) Adjust the pH=7 and heat up to 40°C;

[0049] (4) Add 25 grams of multiple compound proteases (mixed by microbial protease, papain, bromelain, pepsin and trypsin in a ratio of approximately 6: 1: 1: 1: 1) in the biochemical reactor, after stirring, Enzyme hydrolysis reaction 2 to 6 hours;

[0050] (5) Take a sample every 0.5 hours during the enzymolysis process, and use SDS-PAGE or HPLC / MS method to measure the molecular weight distribution of the biochemical reaction products, and determine the end time of the enzymolysis reaction according to the requirements for the molecular weight of the target product;

[0051] (6) The enzyme is inactivated, and the reaction product is filtered and frozen.

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PUM

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Abstract

The present invention is the preparation process of natural active protein and peptide. The natural material with rich protein is processed through defatting, cleaning, homogenizing, mixing with deionized water inside a bioreactor, pH regulating to 5-8, heating to 70-90 deg.c, maintaining and stirring for 10-30 min, quick cooling to 30-50 deg.c and mixing with composite proteinase for enzymolysis for 2-6 hr. During enzymolysis, the reactant is sampled once every 0.5 hr, and the molecular weight distribution of the product is determined in SDS-PAGE or HPLC / MS method. According to the requirement on the molecular weight of target product, the enzymolysis degree is judged, the end time of enzymolysis is determined, enzyme is deactivated immediately after finishing the reaction and the product is filtered and frozen for preservation.

Description

1. Technical field [0001] The invention relates to a preparation method of natural active protein and peptide, and belongs to the field of biological product processing. 2. Background technology [0002] In recent years, the research on natural proteins and peptides has progressed rapidly, and people's own demand for pure natural products has continued to increase. Pure natural substances have become the fashion people pursue, and various products made of pure natural animals and plants have also emerged as the times require. pregnancy. However, the process for preparing natural active proteins and peptides is complicated, the efficiency is low, the stability of the product itself is poor, and the cost remains high, thus restricting the wide application of the product. The reasons for the analysis are as follows: [0003] (1) In terms of structure and function, natural biologically active proteins and peptides are the most complex compounds in nature, so it is not practica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00
Inventor 谢君张义正张强叶勇徐宁舒子斌
Owner 谢君
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