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Klebsiella oxytoca and its application in bioelectricity production

A technology of Klebsiella and microorganisms, which is applied in the application field of Klebsiella oxytoca and its bioelectricity, can solve the problems that the electricity generation characteristics of electron donors have yet to be proven, and achieve reduction requirements and strong The effect of electrochemical activity

Active Publication Date: 2013-07-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that so far, there has been no detailed report on the isolation of Klebsiella sp. electrogenic bacteria directly from the enriched MFC anode biofilm, and the characteristics of electricity generation using different electron donors remain to be ascertained

Method used

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  • Klebsiella oxytoca and its application in bioelectricity production
  • Klebsiella oxytoca and its application in bioelectricity production
  • Klebsiella oxytoca and its application in bioelectricity production

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Screening and isolation of strain CCTCC M 2012446

[0032] Transfer 300mL of sludge from the secondary sedimentation tank of the sewage treatment plant to a 500mL beaker, add 200mL of glucose nutrient solution, stir well, and use NaHCO 3 Adjust the pH value of the solution to about 7.0 (6.7-7.2), and place it in a 30°C incubator for 1 day. Measure the pH and, where appropriate, use NaHCO 3 Adjust the pH of the solution to 6.7-7.2, stir evenly, and discard the supernatant after precipitation. Repeat the culture for 3 days for later use.

[0033] To start a single-chamber air cathode microbial fuel cell, the MFC is a cuboid (length×width×height=8.5×7×6.5cm 3 , effective volume 200mL), the material is 0.5cm plexiglass, one end is closed with a blind plate, and the other end is placed with a splint to place the cathode. The air cathode is platinum-loaded carbon paper (0.5mg cm -2 ), the anode is nitric acid modified carbon felt (BET 1200m 2 g -1 , 2mm), the electrode...

Embodiment 2

[0036] Cyclic Voltammetry Analysis of Strain CCTCC M 2012446

[0037] Inoculate Klebsiella oxytoca Z6 into ferric citrate medium (tryptone 10g L -1 , yeast extract 5g·L -1 , sodium chloride 10g·L -1 , ferric citrate 20mmol·L -1 , pH 7.4), (30±1) ℃ anaerobic culture for 18h. Take the bacterial solution in the mid-logarithmic growth phase, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, and centrifuge the obtained bacterial suspension again according to the above steps, repeat washing twice in this way, and add PBS buffer Make bacterial suspension, OD 600 It is about 0.6. The cyclic voltammetry test was performed on the obtained bacterial suspension with an electrochemical workstation. The test used a three-electrode system, with a glassy carbon electrode as the working electrode, an Ag / AgCl electrode as the reference electrode, and a platinum sheet as the counter electrode. The scan rate was 100mV s -1 , The scanning range i...

Embodiment 3

[0040] Application of Strain CCTCC M 2012446 in Treatment of Various Artificial Wastewater

[0041] The strain CCTCC M 2012446 was inoculated into LB medium, and cultured anaerobically at (30±1)°C for 18 hours. Take the bacterial solution in the mid-logarithmic growth phase, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, and centrifuge the obtained bacterial suspension again according to the above steps, repeat washing twice in this way, and add PBS buffer Make bacterial suspension, OD 600 It is about 0.6.

[0042] Start the single-chamber air cathode microbial fuel cell, the microbial fuel cell is cylindrical (cross-sectional diameter is 2cm, effective volume 6.28cm 3 ), made of polycarbonate. The air cathode is platinum-loaded carbon paper (0.5mg cm -2 ), the anode is nitric acid modified carbon felt, the inside of the cathode is a layer of cation exchange membrane, the electrode spacing is 2cm, and titanium wire is used a...

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Abstract

The invention discloses a Klebsiella oxytoca strain and its application in bioelectricity production. The strain is characterized in that it is Klebsiella oxytoca Z6, which is preserved in China Center for Type Culture Collection (called CCTCC for short) on November 6, 2012 and has a preservation number of CCTCC NO: M 2012446. The strain can transmit electrons to an extracellular electron acceptor under an anaerobic condition. When the strain is inoculated into a microbial fuel cell, it can degrade organic matters and generate electricity simultaneously. Compared with other strains, the Klebsiella oxytoca Z6 strain not only has very strong electrochemical activity, but also can make use of various organic matters as the sole carbon source for electricity generation. Based on the above characteristics, the strain has very good application in environmental pollution remediation and bio-energy recovery.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution biological treatment and bioenergy, and specifically relates to a strain of Klebsiella oxytoca and the application of bioelectricity produced therefrom. Background technique [0002] In recent years, microbial fuel cell (MFC) technology, as an emerging wastewater treatment and pollution remediation technology, has received extensive attention. It directly uses microorganisms to oxidize the organic or inorganic substances in the fuel and generate electrons and protons. The protons and electrons produced are transferred to the cathode through the proton exchange membrane and the external circuit respectively, and react with the final electron acceptor to produce water, which finally converts the organic or inorganic substances in the fuel. Chemical energy of inorganic substances and convert electrical energy. The technology combines the biological treatment of pollution with the rec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20H01M8/16C12R1/22
CPCY02E60/50Y02P70/50
Inventor 朱能武彭月吴平霄
Owner SOUTH CHINA UNIV OF TECH
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