Separation method of butyric acid oxidized hydrogen-producing acetogenic dominant microorganisms

Abstract

The invention discloses a separation method of butyric acid oxidized hydrogen-producing acetogenic dominant microorganisms, relating to a separation method of hydrogen-producing acetogenic bacteria and solving the problems that the traditional separation method is difficult to separate and the hydrogen-producing rate of the cultured hydrogen-producing acetogenic bacteria is low. The separation method comprises the following steps of: firstly, preliminarily domesticating anaerobic activated sludge; secondly, preparing a bacterial suspension A; thirdly, enriching a back bacterial suspension; fourthly, enriching and culturing the enriched back bacterial suspension again; fifthly, preparing compound microorganisms of butyric acid oxidized hydrogen-producing acetogenic bacteria and methanogen; sixthly, preparing the butyric acid oxidized hydrogen-producing acetogenic microorganisms; seventhly, transferring the butyric acid oxidized hydrogen-producing acetogenic microorganisms to a butyric acid culture medium for vibration velocity culture; and eighthly, repeating the step seven for 3-6 times and then separating. The method has easy separation and simple operation, and the hydrogen-producing rate of the separated dominant microorganisms is about 7-10 times of that of the hydrogen-producing bacteria. The advantageous bacterium group not only can degrade the butyric acid with higher concentration, but also can effectively improve the treating efficiency of organic wastewater with high concentration.

Description

technical field [0001] The invention relates to a method for isolating dominant bacterial groups producing hydrogen and acetate. Background technique [0002] Anaerobic biological treatment method is a common technology for high-concentration organic wastewater treatment nowadays. In the process of anaerobic biological treatment method, due to the presence of acid-producing fermentation bacteria (including hydrogen-producing and acetogenic bacteria), and because the ratio of acid-producing fermentation bacteria to The growth and metabolic rate of the hydrogen-producing acetogenic bacteria group is fast, and if a large amount of organic volatile acids such as propionic acid and butyric acid cannot be converted into acetic acid in time, it will not only limit the subsequent methanogenesis, but also due to the accumulation of volatile acids and pH. All microbial groups in the reaction system are severely inhibited, which eventually leads to the failure of the reactor. Therefor...

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of the existing separation method of hydrogen-producing acetogenic bacteria that is difficult to separate and the hydrogen production rate of the cultivated hydrogen-producing acetogenic bacteria is low