Separation method of monoprop-oxidating hydrogen-producing acetogenic dominant bacterial flora

Abstract

The invention relates to a separation method of monoprop-oxidating hydrogen-producing acetogenic dominant bacterial flora, in particular to a separation method of hydrogen-producing acetogenic bacteria, solving the problem of separation difficulty and low hydrogen-producing rate of hydrogen-producing acetogenic bacteria cultured by using the traditional separation method. The separation method comprises the following steps of: (1) primarily training anaerobic active mud; (2) preparing a bacterial suspension A; (3) enriching the bacterial suspension; (4) secondarily enriching and culturing the enriched bacterial suspension; (5) preparing a compound bacterial flora of monoprop-oxidating hydrogen-producing acetogenic bacteria and methane-producing bacteria; (6) preparing a monoprop-oxidating hydrogen-producing acetogenic bacterial flora; (7) transferring the monoprop-oxidating hydrogen-producing acetogenic bacterial flora into a monoprop culture substrate to be cultured; and (8) repeating the step (7) for 4-8 times for separation. The invention method has easy separation and simple operation, and the hydrogen-producing rate of the separated dominant bacterial flora is about 6-8 times that of the traditional hydrogen-producing bacteria. The dominant bacterial flora of the invention not only can degrade higher-concentration monoprop, but also can effectively improve the processing efficiency of high-concentration organic wastewater.

Description

technical field [0001] The invention relates to a method for isolating dominant bacterial groups producing hydrogen and acetate. Background technique [0002] Anaerobic biological treatment method is a common technology for high-concentration organic wastewater treatment nowadays. In the process of anaerobic biological treatment method, due to the presence of acid-producing fermentation bacteria (including hydrogen-producing and acetogenic bacteria), and because the ratio of acid-producing fermentation bacteria to The growth and metabolic rate of the hydrogen-producing acetogenic bacteria group is fast, and if a large amount of organic volatile acids such as propionic acid and butyric acid cannot be converted into acetic acid in time, it will not only limit the subsequent methanogenesis, but also due to the accumulation of volatile acids and pH. All microbial groups in the reaction system are severely inhibited, which eventually leads to the failure of the reactor. Therefor...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of the existing separation method of hydrogen-producing acetogenic bacteria that is difficult to separate and the hydrogen production rate of the cultivated hydrogen-producing acetogenic bacteria is low