Ecological regulating and controlling method for sulfate reducing bacteria liveness in surface water of oil field, and used restrainer thereof
A surface water and sulfate technology, applied in plant growth regulators, biocides, chemical instruments and methods, etc., can solve the problems of low sterilization efficiency, fungicides, etc., achieve wide coverage, low cost, and increase redox potential Effect
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specific Embodiment approach 1
[0008] Specific embodiment 1: In this embodiment, the inhibitor of sulfate-reducing bacteria activity in surface water in the oil field consists of 10-20 parts of denitrifying and nitrifying bacteria liquid, 0.01-0.08 parts of nutrient elements and 0.01-0.05 parts of synergistic Inhibitory substances; the denitrifying bacteria are Bacillus sp, Denitrifyingbacteria, Pseudomonas aeruginosa, Rhizobium sp, Thermomonas sp and Acidovoraxsp; the concentration of bacteria in the denitrifying and nitrifying bacteria liquid is 10 5 ~10 9 Individual / mL; the nutrient element is sodium nitrate, potassium nitrate or sodium nitrite; the synergistic inhibitory substance is ammonium molybdate, sodium molybdate or copper nitrate.
[0009] The role of nutrients in this embodiment is to provide nutrition for denitrifying bacteria, stimulate the growth of denitrifying bacteria and increase the redox potential of oilfield surface water; the role of synergistic inhibitory substances is to specifical...
specific Embodiment approach 2
[0010] Specific embodiment two: what this embodiment is different from specific embodiment one is that the inhibitor of sulfate-reducing bacteria activity in oilfield surface water is by mass fraction ratio by 15 parts of denitrifying and nitrifying bacteria liquids, 0.05 parts of nutrient elements and 0.03 parts made of synergistic inhibitory substances; the denitrifying bacteria are Bacillus sp, Denitrifying bacteria, Pseudomonas aeruginosa, Rhizobium sp, Thermomonas sp and Acidovorax sp; the concentration of bacteria in the denitrifying and nitrifying bacteria liquid is 10 7 Individual / mL; the nutrient element is sodium nitrate, potassium nitrate or sodium nitrite; the synergistic inhibitory substance is ammonium molybdate, sodium molybdate or copper nitrate. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0011] Specific embodiment three: the ecological regulation and control method of sulfate-reducing bacteria activity in the oil field surface water of the present embodiment is carried out according to the following steps: one, preparation inhibitor: Bacillus sp, Denitrifying bacteria, Pseudomonas aeruginosa, Rhizobium sp, Thermomonas sp and Acidovorax sp six A kind of denitrification and nitrification bacteria mixed expansion culture, the concentration of bacteria was 10 5 ~10 9 1 / mL denitrifying and nitrifying bacteria liquid, and then mix 10 to 20 parts of denitrifying and nitrifying bacteria liquid, 0.01 to 0.08 parts of nutrient elements and 0.01 to 0.05 parts of synergistic inhibitory substances according to the ratio of parts by mass to obtain an inhibitor; 2. According to the SO in oilfield surface water 4 2- Inhibitors are added at a molar ratio of 1:0.8-6 to nitrate; wherein the culture medium used in the mixed expansion culture of denitrifying and nitrifying bacte...
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