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Sulfate reducing bacteria with tolerance on arsenic

A sulfate and tolerance technology, applied in the field of environmental microorganisms, can solve problems such as low efficiency, arsenic toxicity, and secondary pollution, and achieve the effects of a wide range of carbon sources, strong growth and metabolism activity, and high conversion efficiency

Inactive Publication Date: 2013-04-03
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, a typical gold mine bioleaching solution contains up to 10g / L arsenic and 20g / L iron. For this acidic arsenic-containing wastewater, the process of adding lime is often used, and the sulfate ion is converted into gypsum, and the arsenic and hydrogen Co-precipitation of iron oxide can be removed. This chemical treatment process has low efficiency in removing arsenic and produces a large amount of solid waste. The treatment of solid waste is likely to cause secondary pollution. Now it is generally believed that hydrogen sulfide and arsenic produced by the metabolism of sulfate-reducing bacteria are used The reaction makes arsenic to orpiment (As 2 S 3 ) is removed from the solution in the form of precipitation is an efficient and non-secondary pollution process
However, high concentrations of arsenic in wastewater have a strong toxic effect on microorganisms. Therefore, in order to ensure the treatment capacity of sulfate-reducing bacteria treatment method for arsenic-containing wastewater, sulfates with high growth and metabolism activity are still required under high concentrations of arsenic. Reducing Bacteria Strains

Method used

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  • Sulfate reducing bacteria with tolerance on arsenic
  • Sulfate reducing bacteria with tolerance on arsenic
  • Sulfate reducing bacteria with tolerance on arsenic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation and identification of the bacterial strain of the present invention.

[0028] (1) Isolation and purification of Enterobacter taihuN3.

[0029] Using liquid culture medium with ferrous sulfate as an indicator, the metabolite hydrogen sulfide of sulfate-reducing bacteria can form ink color with ferrous ions, which can be used as a sign of the enrichment of sulfate-reducing bacteria flora. Use a small-caliber container (500ml serum bottle) with a screw-top stopper, dispense a certain volume of enriched medium and then sterilize it under high pressure. Inoculate the bottom mud of Taihu Lake with 20% of the inoculum until it is full, cover it tightly and culture it in a 30°C incubator, and keep it in the dark for about a week, until the color of the enrichment solution becomes ink-colored. 2 The rotten egg smell of S means that the enrichment is successful.

[0030] The enriched bacterial solution was made into bacterial suspensions with different dilu...

Embodiment 2

[0039] Example 2: Growth characteristics of the strains of the present invention.

[0040] Add 9.8ml medium into the anaerobic tube, inoculate 0.2ml logarithmic phase Enterobacter (Enterobacter) taihuN3 bacterial solution, and culture in an anaerobic box at 30°C in the dark. (The medium formula is the same as the above-mentioned enrichment and separation medium), samples were taken at 0, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70h, 80h, 90h, and 100h after inoculation, and measured The absorbance of the bacterial solution at 600nm. Filter the sample with a 0.22 μm filter membrane, and measure SO in the sample by ion chromatography 4 2- concentration.

[0041] Enterobacter (Enterobacter) taihuN3 growth curve and SO in the medium 4 2- The concentration change curve is as Figure 4 As shown, 10-40 hours after inoculation is the logarithmic growth phase, and 40-80 hours is the stable period. After 80 hours, the absorbance of the bacterial solution decreases, and the bacteria...

Embodiment 3

[0041] Enterobacter (Enterobacter) taihuN3 growth curve and SO in the medium 4 2- The concentration change curve is as Figure 4 As shown, 10-40 hours after inoculation is the logarithmic growth phase, and 40-80 hours is the stable period. After 80 hours, the absorbance of the bacterial solution decreases, and the bacterial body gradually declines. The sulfate content in the culture medium did not change within 20 hours of starting the culture, the sulfate concentration in the medium gradually decreased at 30-80 hours, and the sulfate concentration tended to be stable at 80 hours. The sulfate content in the medium dropped from the initial 1400mg / L to 430mg / L, and the sulfate conversion rate was 70%. The decrease of sulfate content was consistent with the growth of the strain, and the sulfate content of the medium in the stationary phase of the growth of the strain decreased the fastest. Example 3: Utilization of different carbon sources by the strain of the present inventio...

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Abstract

The invention discloses sulfate reducing bacteria with tolerance on arsenic. The sulfate reducing bacteria are named as enterobacteria (enterobacter sp.) taihuN3 in classification, wherein the bacterial strain is preserved in General Microbiology Centre of China Committee for Culture Collection of Microorganisms; the preservation number is 23 Nov 2012; and the preservation number is CGMCC No.6886. Application of the sulfate reducing bacteria with tolerance on arsenic in wastewater treatment is also disclosed by the invention. The bacterial strain disclosed by the invention is wide in utilizable carbon source range, easy to cultivate, strong in growth metabolic activity, and high in sulphate conversion efficiency. The bacterial strain disclosed by the invention can normally grow when the concentration is 10-1000mg / LAs(V); and the percent reduction of the bacterial strain disclosed by the invention under 200mg / LAs(V) of sulfate achieves 50%. The sulfate reducing bacteria can be used for processing high-concentration arsenic wastewater, and has good application prospect.

Description

technical field [0001] The invention relates to a sulfate-reducing bacterium, which belongs to the field of environmental microorganisms. Background technique [0002] Sulfate reducing bacteria (SRB) are a class of prokaryotic microorganisms that use organic or inorganic compounds as electron donors to reduce sulfate to produce sulfide, with diverse phylogenetic branches and physiological characteristics. Sulfate-reducing bacteria produce sulfide through dissimilatory sulfate reduction under anaerobic conditions. This process consumes sulfate radicals in the solution and can be used to treat sulfate wastewater; the negative charge on the cell surface of sulfate-reducing bacteria and the secreted Extracellular matter has a strong electrostatic adsorption and bioflocculation effect on heavy metal ions; while metabolizing sulfate ions, it will consume hydrogen ions, reduce the acidity of the solution, and cause metal ions to form hydroxide precipitates, which will produce a lar...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10C02F101/20
Inventor 肖琳刘莹
Owner NANJING UNIV
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