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A kind of enrichment culture method of heterotrophic nitrifying bacteria

A technology of enrichment culture and heterotrophic nitrification, which is applied in the field of environmental microorganisms, can solve problems such as complex processes, and achieve the effects of process simplification, accelerated cell growth, and improved ability

Active Publication Date: 2020-06-09
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN201010510855.0 discloses a screening method for nitrogen-removing bacteria, which is divided into processes such as enrichment, purification, and domestication. In actual application, compounding of individual strains is required, and the process is relatively complicated

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  • A kind of enrichment culture method of heterotrophic nitrifying bacteria

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preparation example Construction

[0021] The microbial growth promoter involved in the present invention can be prepared according to the methods described in CN201410585430.4, CN201410585449.9 and CN201410585485.5. Three types of growth promoters were prepared according to the ratio and formula of the promoters in Table 1 by the above method, and the concentrations of the promoters were all 0.5 g / L.

[0022] Table 1 Formula and ratio of accelerator

[0023]

Embodiment 1

[0025]First, inoculate the activated sludge rich in nitrifying bacteria from a sewage treatment plant into the aeration reactor. After inoculation, the MLSS is 3000mg / L, and the temperature is 25°C, pH7.5, DO (dissolved oxygen) 2.5mg / L. , the method of gradually increasing the concentration of ammonia nitrogen in the matrix is ​​used for enrichment. The initial concentration of ammonia nitrogen in the sewage is 100mg / L, and the concentration of COD is 150mg / L. Concentration increased by 50mg / L than last time until it reached 500mg / L. Then take a certain amount of enriched sludge and put it into a facultative reactor to cultivate heterotrophic nitrifying bacteria under the conditions of DO: 0.5mg / L, temperature 25°C, and pH 7.5. The culture medium was replaced once a day, and the microbial growth promoter I was added once a day according to the concentration of the promoter in the culture system at 25 mg / L during the culture process. The concentration of ammonia nitrogen in th...

Embodiment 2

[0027] First, inoculate the activated sludge rich in nitrifying bacteria from a sewage treatment plant into the aeration reactor. After inoculation, the MLSS is 3000mg / L. Under the conditions of temperature 30°C, pH 7.8, DO (dissolved oxygen) 5mg / L, Enrichment is carried out by gradually increasing the concentration of ammonia nitrogen in the matrix. The initial concentration of ammonia nitrogen in the sewage is 150mg / L, and the concentration of COD is 200mg / L. Increase by 100mg / L than last time until it reaches 600mg / L. Then take a certain amount of enriched sludge and put it into a facultative oxygen reactor to cultivate heterotrophic nitrifying bacteria under the conditions of DO: 0.5mg / L, temperature 30°C, and pH 7.8. The culture medium was replaced once a day, and the growth promoter II was added every day according to the concentration of the promoter in the culture system at 30 mg / L during the cultivation process. The concentration of ammonia nitrogen in the culture me...

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Abstract

The invention discloses an enrichment culture method of heterotrophic nitrobacteria. The method comprises (1) using ammonia containing sewage to carry out enrichment culture of denitrifying bacterium floras in aerobic active sludge; and (2) using a microbial growth promoter to culture heterotrophic floras under a low dissolved oxygen condition, wherein the promoter comprises 40 to 100 parts by weight of metal salts composed of calcium salts, copper salts, magnesium salts, and / or ferrous salts, 5 to 30 parts by weight of polyamine, and 0.5 to 15 parts by weight of organic acid hydroxylamine. According to the enrichment culture method, a growth promoter is added to screen heterotrophic nitrobacteria and promote the growth of heterotrophic nitrobacteria; the promoter has the advantages of simple formula and easy preparation; after addition, heterotrophic nitrobacteria with a natural growth advantage can be enriched quickly, and the heterotrophic nitrobacteria are applied to the sewage denitration treatment. The heterotrophic nitrobacteria can be added into a sewage processing treatment, which is impacted, to rapidly recover the processing performance of the system under different environmental conditions.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a method for enriching and cultivating heterotrophic nitrifying bacteria. Background technique [0002] The traditional biological nitrogen removal process is mostly carried out by two types of bacteria in two different reactors, resulting in a large system footprint and weak impact resistance. Simultaneous nitrification and denitrification (SND) denitrification can simultaneously carry out nitrification and denitrification to remove nitrogen and total nitrogen in the same reactor. This new biological denitrification process not only overcomes some problems existing in the traditional biological denitrification process, but also reduces It has outstanding advantages in terms of energy consumption and material consumption: it can effectively maintain the pH stability in the reactor, reduce or cancel the addition of alkalinity; reduce the volume of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 高会杰孙丹凤郭志华赵胜楠
Owner CHINA PETROLEUM & CHEM CORP
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