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Nitrous acid-type denitrification bacteria strain and application thereof

A short-range denitrification and bacterial strain technology, applied in the field of environmental microorganisms, can solve problems such as Arthrobacter, which has not yet been seen, and achieve the effects of saving carbon sources, fast system startup, and broad application prospects

Active Publication Date: 2012-05-23
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN200910021020.7 discloses a preparation method of a water quality improvement microecological preparation for reducing ammonia nitrogen and nitrite nitrogen, which involves an Arthrobacter strain CGMCC NO.1282, but the microecological preparation of the invention belongs to the technical field of aquaculture technology and ecological environment protection , there is no report on the use of Arthrobacter to treat nitrite nitrogen in industrial wastewater

Method used

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  • Nitrous acid-type denitrification bacteria strain and application thereof
  • Nitrous acid-type denitrification bacteria strain and application thereof
  • Nitrous acid-type denitrification bacteria strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Enrichment culture and separation and purification of the bacteria of embodiment 1

[0027] 1. Enrichment culture of denitrifying bacteria

[0028] The activated sludge in the nitrification tank is taken, and the culture medium containing nitrite is used for enrichment and cultivation under the condition of low dissolved oxygen for a certain period of time to obtain the denitrification activated sludge. During the enrichment process, fresh culture medium was replenished in due course.

[0029] Enriched medium formula: NaNO 2 , methanol, also contains Fe 2+ , Mg 2+ 、K + , Ca 2+four metal cations. The culture conditions are: temperature 30°C; pH 8.2, DO (dissolved oxygen) 0.2-1.0mg L -1 .

[0030] The molar configuration ratio of the four metal cations is 1:(4-8):(5-15):(1-5). During the cultivation process, a growth promoter with a total metal cation concentration of 0.15 mol / L was added every day, and the amount added was 0.1% of the volume of the material in t...

Embodiment 2

[0034] Example 2 Investigation of denitrification ability of bacterial strains of the present invention

[0035] Firstly, single colony FDN-1 was picked from the plate and inoculated into beef extract peptone medium at 30°C and 120r / min on a shaker for 42 hours, after which the concentration of bacteria reached the maximum, and the cultivation was terminated. After the bacterial suspension was centrifuged, it was transferred to the sodium nitrite-methanol culture solution, and the denitrification ability was investigated by batch feeding under the same conditions. Table 1 shows the concentration of nitrite nitrogen and COD in the reaction system after adding materials, and the denitrification effect is shown in figure 2 .

[0036] Table 1 Supplementary material situation

[0037]

[0038]

[0039] From figure 2 The change trend of nitrite nitrogen concentration over time can be seen. The first three batches experienced 9 days, 5 days and 2 days respectively, and the...

Embodiment 3

[0040] Embodiment 3 Application of bacterial strains of the present invention in nitrogen fertilizer wastewater denitrification process

[0041] Get the bacterium suspension that a certain amount of embodiment 2 obtains and inoculate according to the inoculum size of 15%, at temperature 28 ℃, pH be 7.7, DO (dissolved oxygen) is 0.2~0.9mg L -1 Under certain conditions, the effluent of the short-range nitrification reaction of ammonia-containing wastewater produced in the process of treating nitrogen fertilizer production, the water: NO 2 - - The average of N is 400mg / L, and the average of COD is 800mg / L. After treatment, the effluent NO 2 - -N and COD concentration are 0.2mg / L~5.7mg / L and 38mg / L~49mg / L respectively, NO 2 - -N and COD removal rates are greater than 99% and 93% respectively.

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Abstract

The invention relates to a bacteria strain for short-cut denitrification and an application thereof. The short-cut denitrification bacteria strain is arthrobacter creatinolyticus FDN-1 and is preserved in Ordinary Microorganism Center of China Committee for Culture Collection of Microorganisms on March 11, 2010, and the preservation number is CGMCC No. 3657. The bacteria strain can directly adopt nitrite nitrogen as a substrate to complete the short-cut denitrification process. When the arthrobacter creatinolyticus FDN-1 is used for processing ammonia-contained waste water, the technique is simple, high denitrification activity can be still obtained under the condition that low-concentration organic carbon source exists, and at the same time the high-concentration organic carbon source can be withstood. When the bacteria strain is in use, the system is rapid to start, and a vast application prospect can be realized in the denitrification treatment process of different types of waste water.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a high-efficiency nitrous acid type denitrification bacterial strain. The denitrification bacterial strain of the invention can carry out denitrification with nitrous nitrogen as a nitrogen source, and is especially suitable for denitrification in the process of short-range nitrification and denitrification of ammonia-containing wastewater. Background technique [0002] The traditional biological denitrification process mainly includes two processes of nitrification and denitrification. Nitrification is the premise of the denitrification process, and the denitrification process can truly remove nitrogen-containing compounds in wastewater. Because nitrifying bacteria have strong aerobic properties, the nitrification process is aerobic; while denitrifying bacteria are generally facultative bacteria, which use oxygen as an electron acceptor under aerobic condi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/28
Inventor 高会杰李志瑞黎元生许谦刘忠生
Owner CHINA PETROLEUM & CHEM CORP
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