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Bacillus amyloliquefaciens and application thereof on aquiculture

A technology for dissolving starch spores and bacilli, which is applied in the field of agricultural microorganism applications to achieve the effect of improving aquaculture environment

Inactive Publication Date: 2010-08-04
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the bacillus used in aquaculture is mainly Bacillus subtilis, and there is no patent for using Bacillus amyloliquefaciens in aquaculture water purification, and the strain has a wide range of tolerance to temperature and pH, and it is easy to grow and reproduce after being put into water. The water purification effect is good, and it can be used to restore aquaculture water bodies

Method used

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  • Bacillus amyloliquefaciens and application thereof on aquiculture
  • Bacillus amyloliquefaciens and application thereof on aquiculture
  • Bacillus amyloliquefaciens and application thereof on aquiculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isolation, screening and cultivation of strains

[0020] Sampling: At three different sampling points in the polluted river ditch, remove about 5mm thick surface mud from the bottom sludge, put the mud into a beaker, seal the mouth with plastic wrap and bring it back to the room for rapid separation.

[0021] Separation: Dilute the sludge with sterile water at a ratio of 1:4, treat it in a water bath at 80°C for 15 minutes, draw 100 μL of the sludge, spread it on a starch medium plate, and incubate at 37°C.

[0022] Purification: After 2 days of plate culture, select the strains that produce hydrolysis circles around them and streak them on the NA medium, and select dry single colonies with irregular, irregular colony edges, and rough surfaces to continue streak culture. After streak culture for 5 generations That is, pure strains were obtained.

[0023] Screening: Spot the purified strains on the starch medium, measure the colony size (C) and the size of the hydrolysi...

Embodiment 2

[0026] Identification characteristics of strains

[0027] 1. Morphological observation and staining reaction of bacteria

[0028] On the broth medium, the colonies are close to cream color, opaque, wrinkled after 2 days of culture, and the edges are irregular; under the microscope, they are rod-shaped (0.7-0.8μm×2-3μm), Gram staining is positive, and the spores are oval Medial, flagella lateral.

[0029] 2. Physiological and biochemical characteristics of the strain

[0030] The strain was inoculated on a Biolog Microplate GP plate and cultured for 24 hours to detect its carbon source utilization. The results are shown in Table 1.

[0031] Table 1 The utilization of carbon source by bacterial strains

[0032] α-Cyclodextrin

[0033] Inulin

[0034] As can be seen from Table 1, strains can utilize the following types of 95 carbon sources: β-Methyl-D-Glucoside, α-Methyl-D-Glucoside, α-D-Glucose, Tween 80, Tween 40, Turanose, Sucrose , N-Acetyl-D-Glucosami...

Embodiment 3

[0078] The water purification function of embodiment 3 bacterial strains

[0079] The simulated aquaculture water is configured indoors, and centrifuged bacterial cells are added to the tank so that the bacteria content in the water is 10 7 CFU·L -1 On the day of adding bacteria and on the 1st, 2nd, 3rd, 6th, and 7th days after adding bacteria, take water and immediately take water to measure the content of nitrite and nitrate in the water.

[0080] From figure 2 and image 3 It can be seen that the bacteria can basically completely degrade nitrite nitrogen and nitrate nitrogen in the water on the sixth day of the test.

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Abstract

The invention relates to Bacillus amyloliquefaciens and application thereof on aquiculture. The bacterial strain is separated from the substrate sludge of a polluted brook, is identified as the Bacillus amyloliquefaciens and is collected in Microscobial Culture Collection of China. The bacterial strain can normally grow within the temperature of 20 to 45 DEG C and within the pH value of 5.0 to 10.0; the bacterial strain can reach the fermentation terminal point when being inoculated on a broth culture medium the initial pH value of which is 7.0 according to inoculum concentration of 1:20 and cultured for 18 hours at the speed of 150r.min-1 at the temperature of 39 DEG C. The invention is characterized in that the bacterial strain can enable the content of nitrate nitrogen, nitrite nitrogen, COD and the like in a culture water body to be obviously lowered, has no harm to people and livestock and can be produced on a large scale for improving the aquiculture environment. The Bacillus amyloliquefaciens has obvious degradation function on main pollutants in a simulate culture water body and can be made into a micro-ecology preparation for purifying the culture water body.

Description

technical field [0001] The invention belongs to the application field of agricultural microorganisms, and relates to a newly screened bacillus amyloliquefaciens and its application in aquaculture. Background technique [0002] my country is one of the countries with the longest history of aquaculture in the world, with a relatively large area of ​​aquaculture, which was 6.65 million hectares in 2001. With the rapid development of the aquaculture industry, the degree of intensification continues to increase, and the physical and chemical environment and ecological environment of the aquaculture water body are deteriorating day by day; at the same time, the discharge of industrial wastewater and domestic sewage has also exacerbated the decline in the quality of the aquaculture water body; these problems have caused increasingly serious aquaculture diseases , the loss is increasing, and it is estimated that the annual loss of aquaculture products caused by diseases in the whole...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/68C12Q1/04C02F3/34C12R1/07
Inventor 谢凤行赵玉洁张峰峰周可李亚玲
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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