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Composite microecological preparation and preparation method thereof

A technology of compound microecology and preparation, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as overfilling, improper post-processing, and small environmental adaptation range of strains, so as to improve immune activity and prevent pollution Strong ability and good product stability

Active Publication Date: 2016-04-06
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] At present, there are many similar products on the market, but there are still many deficiencies. For example, the function of the microbial strains in the product is not efficient; The effect is not good; the liquid submerged fermentation technology is unstable, and the product consistency is poor; the post-processing is improper, and the product maintenance is poor, etc.

Method used

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  • Composite microecological preparation and preparation method thereof
  • Composite microecological preparation and preparation method thereof
  • Composite microecological preparation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The acquisition of embodiment 1 symbiotic strain combination

[0035] All the following strains are considered as candidate strains for the research of microecological preparations: (1) Newly preserved strains with excellent functions in our laboratory: Bacillus subtilis ( Bacillus subtilis ) CGMCCNo.9356 and Enterococcus faecium ( Enterococcus faecium ) CGMCCNo.9134; (2) The laboratory has preserved excellent strains including: Bacillus licheniformis (Bacillus licheniformis) CGMCCNo.7030; Saccharomyces cerevisiae ( Saccharomyces cerevisiae) CGMCCNo.6921; Lactobacillus pumilus ( Lactobacillus brevis ) CGMCCNo.4756; Lactobacillus johnsonii ( Lactobacillus johnsonii ) CGMCCNo.4926, Lactobacillus acidophilus (Lactobacillus acidophilus) CGMCCNo.6499; Pediococcus lactis ( Pediococcus acidilactic) CGMCCNo.6500; (3) Commercially available strains: the following strains were isolated from commercially available single-strain powder: 1 strain of Lactococcus lactis, i...

Embodiment 29

[0038] Embodiment 29 bacterial strains liquid mixed culture

[0039] The symbiotic bacterial strain combination of 9 strains was screened, and after the 9 strains were cultured in the same medium YH, 1 mL of the seed solution of each strain with the same concentration was taken, and mixed into a certain volume of liquid medium YH for mixed culture. The fermentation temperature 33°C, shaker speed 100rpm, initial pH value 7.0, continuous culture for 4 days, record the number of live bacteria on 0, 3 and 4 days, the results are shown in Table 1: after 4 days of mixed culture of 9 strains, the total concentration of bacillus reached 1.4×10 12 CFU / mL or more, the total concentration of lactic acid bacteria is 6.08×10 11 CFU / mL or more, the yeast concentration is 6.52×10 8 More than CFU / mL, it shows that the 9 strains grow well together.

[0040] Table 1

[0041]

Embodiment 39

[0042] Example 39 bacterial seed liquid preparation

[0043] (1) Preparation of seed solution of 4 strains of Bacillus

[0044] Bacillus subtilis H8-1, the preservation number is CGMCCNo.9356; Bacillus megaterium; Bacillus licheniformis (Bacillus licheniformis) CGMCCNo.5094; single colonies of Bacillus amyloliquefaciens CGMCCNo.7030 were inoculated into 250mL Erlenmeyer flasks containing 100ml of MNB medium, the shaker speed was 180rpm, and cultured in a constant temperature shaker at 33°C for 16h; the prepared primary seeds solution, with an OD600 of about 0.9, inoculate 1.0% of the inoculum into a 1L Erlenmeyer flask containing 500mL of MNB medium, culture in a constant temperature shaker at 33°C for 12 hours, the shaker speed is 180rpm, and the OD600 is about 0.8;

[0045] (2) Preparation of 4 strains of lactic acid bacteria seed solution

[0046] Enterococcus faecium, the preservation number is CGMCCNo.9134; Lactobacillus pumilus ( Lactobacillus brevis ) CGMCC No.4756...

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Abstract

The invention provides a composite active bacterial preparation which is used for aquatic products and has the water improving and fertilizing functions and a preparation method thereof by applying a modern bioengineering technology mainly for the problem that in existing aquaculture, the water quality of a pond is poor. The composite microecological preparation is prepared by mixing bacillus subtilis, bacillus megatherium, bacillus amyloliquefaciens, bacillus licheniformis, enterococcus faecium, lactobacillus brevis, lactobacillus plantarum, lactococcus lactis and saccharomyces cerevisiae through liquid-submerged fermentation. In the liquid preparation, microorganisms are in a dormant state and are stable and easy to preserve, after the preparation is put into water, the dormant strains can quickly resuscitate, harmful substances such as nitrite nitrogen and ammonia nitrogen in the water are quickly decomposed, good algae growth is facilitated, harmful algae reproduction is inhibited, and therefore the purpose of improving the water quality of the culture water is achieved.

Description

technical field [0001] The invention belongs to the technical field of aquatic micro-ecological preparations, and in particular relates to a micro-ecological preparation and a preparation method thereof. Background technique [0002] In recent years, the high-density and intensive aquaculture model has easily broken the original ecological balance of the pond: harmful drug-resistant bacteria and harmful algae multiply; the microorganisms in the pond that decompose harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide are insufficient, The accumulation of harmful substances such as ammonia nitrogen and nitrite in water bodies will eventually affect the health and growth quality of farmed animals in water bodies. Therefore, the balance of bacterial phase and algal phase in the aquaculture environment and the content of harmful substances in water are three major problems to be solved in aquaculture production. In aquaculture production, the proportion of p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C02F3/34C12R1/125C12R1/11C12R1/10C12R1/07C12R1/25C12R1/24C12R1/865C12R1/01
Inventor 胡婷马青山贠锦军何增国
Owner BEIJING DABEINONG TECH GRP CO LTD
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