A kind of probiotics capable of degrading ochratoxin and its preparation method
A micro-ecological preparation, ochratoxin technology, applied in the biological field, can solve problems such as the inability to prevent ochratoxin pollution, the inability to prevent feed pollution from the source, and limit the application of Clostridium butyricum microorganisms, so as to achieve stable product properties and improve Stability, effect of relieving flatulence and colic
Examples
Embodiment 1
[0024] Example 1 A kind of probiotics capable of decomposing ochratoxin
[0025] The probiotics include the following raw materials in parts by weight:
[0026] 40 parts of Clostridium butyricum, 20 parts of Bacillus natto, 20 parts of Bacillus coagulans, 5 parts of FOMESOFFICINALIS extract, 0.1 part of sodium butyrate, 100 parts of liquid medium;
[0027] The liquid culture medium is that 1L medium consists of the following components: 10 parts of glucose, 25 parts of protein powder, 5 parts of sodium deoxycholate, 50 parts of brown sugar, 0.1 part of vitamin B1, 0.1 part of vitamin B2, vitamin B12 0.05 parts, 1 part of sodium chloride, 2 parts of potassium dihydrogen phosphate, 50 parts of sterile distilled water; the number of viable bacteria of Clostridium butyricum is 1Γ10 10 CFU / mL;
[0028] The preparation method of the probiotics for decomposing ochratoxin comprises the following steps: inserting Bacillus natto strains into the microbial liquid culture medium, aerat...
Embodiment 2
[0029] Example 2 A kind of probiotics capable of decomposing ochratoxin
[0030] The probiotics include the following raw materials in parts by weight:
[0031] 50 parts of Clostridium butyricum, 10 parts of Bacillus natto, 15 parts of Bacillus coagulans, 8 parts of FOMESOFFICINALIS extract, 2.7 parts of sodium butyrate, 150 parts of liquid medium;
[0032] The liquid culture medium is that 1L medium consists of the following components: 18 parts of glucose, 16 parts of protein powder, 3 parts of sodium deoxycholate, 80 parts of brown sugar, 2.5 parts of vitamin B1, 0.75 parts of vitamin B2, vitamin B12 0.03 parts, 2 parts of sodium chloride, 1.5 parts of potassium dihydrogen phosphate, 750 parts of sterile distilled water; the number of viable bacteria of the Clostridium butyricum is 5Γ10 9 CFU / mL;
[0033] The preparation method of the probiotics for decomposing ochratoxin comprises the following steps: inserting the Bacillus natto strain into the microbial liquid cultur...
Embodiment 3
[0034] Example 3 A kind of probiotics capable of decomposing ochratoxin
[0035] The probiotics include the following raw materials in parts by weight:
[0036] 60 parts of Clostridium butyricum, 6 parts of Bacillus natto, 30 parts of Bacillus coagulans, 10 parts of FOMESOFFICINALIS extract, 4 parts of sodium butyrate, 200 parts of liquid medium;
[0037] The liquid culture medium is that 1L medium consists of the following components: 25 parts of glucose, 10 parts of protein powder, 1 part of sodium deoxycholate, 100 parts of brown sugar, 5 parts of vitamin B1, 0.1 part of vitamin B2, vitamin B12 0.02 parts, 3 parts of sodium chloride, 1 part of potassium dihydrogen phosphate, 1000 parts of sterile distilled water; the number of viable bacteria of Clostridium butyricum is 1Γ10 9 CFU / mL;
[0038] The preparation method of the probiotics for decomposing ochratoxin comprises the following steps: inserting Bacillus natto strains into the microbial liquid culture medium, aeratio...
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Description
Claims
Application Information
- IPC
- A23K10/18; A23K10/16; A23L5/20; C12N1/20; C12R1/07; C12R1/145
- Inventors
- ηδΏι; η¨ζζ₯