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A kind of probiotics capable of degrading ochratoxin and its preparation method

A micro-ecological preparation, ochratoxin technology, applied in the biological field, can solve problems such as the inability to prevent ochratoxin pollution, the inability to prevent feed pollution from the source, and limit the application of Clostridium butyricum microorganisms, so as to achieve stable product properties and improve Stability, effect of relieving flatulence and colic

Active Publication Date: 2021-12-31
HUBEI XIPU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent document CN105767507A discloses a kind of probiotics for feed, antibiotic-free feed and its preparation method. Although the probiotics can decompose the anti-nutritional substances in the feed, improve the utilization rate of the feed, inhibit the growth of pathogenic bacteria, and improve the immunity of animals effect, but cannot prevent ochratoxin contamination
Patent document CN106616233A discloses a premixed feed capable of degrading mycotoxins, its preparation method and pig rations. Although the premixed feed can reduce the mycotoxins in the feed, it still cannot prevent the contamination of ochratoxins.
[0005] At present, Clostridium butyricum has been allowed to be added to feed as a feed additive, and as a substitute product for anti-diarrhea antibiotics, it has played an anti-resistance effect in antibiotic-free farming, but due to the inability to prevent feed pollution from the source, restrictions The application of various microorganisms such as Clostridium butyricum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 A kind of probiotics capable of decomposing ochratoxin

[0025] The probiotics include the following raw materials in parts by weight:

[0026] 40 parts of Clostridium butyricum, 20 parts of Bacillus natto, 20 parts of Bacillus coagulans, 5 parts of FOMESOFFICINALIS extract, 0.1 part of sodium butyrate, 100 parts of liquid medium;

[0027] The liquid culture medium is that 1L medium consists of the following components: 10 parts of glucose, 25 parts of protein powder, 5 parts of sodium deoxycholate, 50 parts of brown sugar, 0.1 part of vitamin B1, 0.1 part of vitamin B2, vitamin B12 0.05 parts, 1 part of sodium chloride, 2 parts of potassium dihydrogen phosphate, 50 parts of sterile distilled water; the number of viable bacteria of Clostridium butyricum is 1×10 10 CFU / mL;

[0028] The preparation method of the probiotics for decomposing ochratoxin comprises the following steps: inserting Bacillus natto strains into the microbial liquid culture medium, aerat...

Embodiment 2

[0029] Example 2 A kind of probiotics capable of decomposing ochratoxin

[0030] The probiotics include the following raw materials in parts by weight:

[0031] 50 parts of Clostridium butyricum, 10 parts of Bacillus natto, 15 parts of Bacillus coagulans, 8 parts of FOMESOFFICINALIS extract, 2.7 parts of sodium butyrate, 150 parts of liquid medium;

[0032] The liquid culture medium is that 1L medium consists of the following components: 18 parts of glucose, 16 parts of protein powder, 3 parts of sodium deoxycholate, 80 parts of brown sugar, 2.5 parts of vitamin B1, 0.75 parts of vitamin B2, vitamin B12 0.03 parts, 2 parts of sodium chloride, 1.5 parts of potassium dihydrogen phosphate, 750 parts of sterile distilled water; the number of viable bacteria of the Clostridium butyricum is 5×10 9 CFU / mL;

[0033] The preparation method of the probiotics for decomposing ochratoxin comprises the following steps: inserting the Bacillus natto strain into the microbial liquid cultur...

Embodiment 3

[0034] Example 3 A kind of probiotics capable of decomposing ochratoxin

[0035] The probiotics include the following raw materials in parts by weight:

[0036] 60 parts of Clostridium butyricum, 6 parts of Bacillus natto, 30 parts of Bacillus coagulans, 10 parts of FOMESOFFICINALIS extract, 4 parts of sodium butyrate, 200 parts of liquid medium;

[0037] The liquid culture medium is that 1L medium consists of the following components: 25 parts of glucose, 10 parts of protein powder, 1 part of sodium deoxycholate, 100 parts of brown sugar, 5 parts of vitamin B1, 0.1 part of vitamin B2, vitamin B12 0.02 parts, 3 parts of sodium chloride, 1 part of potassium dihydrogen phosphate, 1000 parts of sterile distilled water; the number of viable bacteria of Clostridium butyricum is 1×10 9 CFU / mL;

[0038] The preparation method of the probiotics for decomposing ochratoxin comprises the following steps: inserting Bacillus natto strains into the microbial liquid culture medium, aeratio...

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PUM

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Abstract

The invention provides a microecological preparation for decomposing ochratoxin and a preparation method thereof. The microecological preparation includes Clostridium butyricum, Bacillus natto, Bacillus coagulans, extract of FOMESOFFICINALIS, sodium butyrate, liquid culture medium and the like. The micro-ecological preparation that can decompose ochratoxin provided by the invention has stable properties, can decompose the pollution caused by ochratoxin in feed, has strong anti-pollution ability, can significantly increase gastrointestinal motility, enhance gastric motility, and relieve gastrointestinal discomfort or bloating, to regulate flatulence.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a microecological preparation for degrading ochratoxin and a preparation method thereof. Background technique [0002] Clostridium butyricum is mainly distributed in human feces and soil, and its anaerobic cultured filtrate contains less fatty acids, which has a strong intestine-regulating effect, which can inhibit pathogenic bacteria in the intestine and promote intestinal Growth of beneficial bacteria such as bifidobacteria and lactobacilli. Clostridium butyricum not only promotes the proliferation and development of beneficial intestinal flora, inhibits the growth and reproduction of harmful bacteria and spoilage bacteria in the intestinal tract of animals, corrects the disorder of intestinal flora, and reduces the occurrence of enterotoxins, but also in B vitamins, vitamin K, amylase and other substances can be produced in the intestinal tract of animals, which have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/18A23K10/16A23L5/20C12N1/20C12R1/07C12R1/145
Inventor 王俊青程林春崔志英李天碧
Owner HUBEI XIPU BIOTECH
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