A kind of compound probiotics and preparation method thereof

A technology of composite microecology and preparation, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as improper post-processing, overfilling, and small environmental adaptability of strains, so as to achieve good product stability and improve Strong immune activity and anti-pollution ability

CN105462872BActive Publication Date: 2019-08-13BEIJING DABEINONG TECH GRP CO LTD +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2019-08-13

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Abstract

The invention provides a composite active bacterial preparation which is used for aquatic products and has the water improving and fertilizing functions and a preparation method thereof by applying a modern bioengineering technology mainly for the problem that in existing aquaculture, the water quality of a pond is poor. The composite microecological preparation is prepared by mixing bacillus subtilis, bacillus megatherium, bacillus amyloliquefaciens, bacillus licheniformis, enterococcus faecium, lactobacillus brevis, lactobacillus plantarum, lactococcus lactis and saccharomyces cerevisiae through liquid-submerged fermentation. In the liquid preparation, microorganisms are in a dormant state and are stable and easy to preserve, after the preparation is put into water, the dormant strains can quickly resuscitate, harmful substances such as nitrite nitrogen and ammonia nitrogen in the water are quickly decomposed, good algae growth is facilitated, harmful algae reproduction is inhibited, and therefore the purpose of improving the water quality of the culture water is achieved.
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Description

technical field

[0001] The invention belongs to the technical field of aquatic micro-ecological preparations, and in particular relates to a micro-ecological preparation and a preparation method thereof. Background technique

[0002] In recent years, the high-density and intensive aquaculture model has easily broken the original ecological balance of the pond: harmful drug-resistant bacteria and harmful algae multiply; the microorganisms in the pond that decompose harmful substances such as ammonia nitrogen, nitrite and hydrogen sulfide are insufficient, The accumulation of harmful substances such as ammonia nitrogen and nitrite in water bodies will eventually affect the health and growth quality of farmed animals in water bodies. Therefore, the balance of bacterial phase and algal phase in the aquaculture environment and the content of harmful substances in water are three major problems to be solved in aquaculture production. In aquaculture production, the proportion of p...

Examples

Embodiment 1

[0034] The acquisition of embodiment 1 symbiotic strain combination

[0035] All the following strains are considered as candidate strains for the research of microecological preparations: (1) Newly preserved strains with excellent functions in our laboratory: Bacillus subtilis ( Bacillus subtilis ) CGMCC No.9356 and Enterococcus faecium ( Enterococcus Faecium ) CGMCC No.9134; (2) The laboratory has preserved excellent strains including: Bacillus licheniformis (Bacillus licheniformis)CGMCC No.7030; Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) CGMCCNo.6921; Lactobacillus pumilus ( Lactobacillus brevis ) CGMCC No.4756; Lactobacillus johnsonii ( Lactobacillus johnsonii ) CGMCC No.4926, Lactobacillus acidophilus (Lactobacillus acidophilus) CGMCC No.6499; Pediococcus lactis ( Pediococcus acidilactic) CGMCC No.6500; (3) Commercially available strains: the following strains were isolated from commercially available single-strain powder: 1 strain of Lactococcu...

Embodiment 2

[0038] Embodiment 2 9 strains of bacteria liquid mixed culture

[0039] The symbiotic bacterial strain combination of 9 strains was screened, and after the 9 strains were cultured in the same medium YH, 1 mL of the seed solution of each strain with the same concentration was taken, and mixed into a certain volume of liquid medium YH for mixed culture. The fermentation temperature 33°C, shaker speed 100rpm, initial pH value 7.0, continuous culture for 4 days, record the number of live bacteria on 0, 3 and 4 days, the results are shown in Table 1: after 4 days of mixed culture of 9 strains, the total concentration of bacillus reached 1.4 ×10 12 CFU / mL or more, the total concentration of lactic acid bacteria is 6.08×10 11 CFU / mL or more, the yeast concentration is 6.52×10 8 More than CFU / mL, it shows that the 9 strains grow well together.

[0040] Table 1

[0041]

Embodiment 3

[0042] Example 3 Preparation of 9 strains of bacterial seed liquid

[0043] (1) Preparation of seed solution of 4 strains of Bacillus

[0044] Bacillus subtilis H8-1, the preservation number is CGMCC No.9356; Bacillus megaterium; Bacillus licheniformis (Bacillus licheniformis) CGMCC No.5094; single colonies of Bacillus amyloliquefaciens CGMCC No.7030 were inoculated into 250mL Erlenmeyer flasks containing 100ml of MNB medium, the shaker speed was 180rpm, and cultured in a constant temperature shaker at 33°C for 16h; the prepared The first-grade seed solution, with an OD600 of about 0.9, was inoculated into a 1L Erlenmeyer flask containing 500mL of MNB medium according to the inoculum amount of 1.0%, and cultivated in a constant temperature shaker at 33°C for 12h, with a shaker speed of 180rpm, and an OD600 of about 0.8 ;

[0045] (2) Preparation of 4 strains of lactic acid bacteria seed solution

[0046] Enterococcus faecium, the preservation number is CGMCC No.9134; Lacto...