Bacterial strain used for short-cut denitrification for nitrogen removal and its application

A nitrification denitrification bacterial strain and nitrification denitrification technology are applied in the field of high-efficiency nitrous acid denitrification strains to achieve the effects of simple process, fast start-up and broad application prospects

Active Publication Date: 2012-05-23
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the use of Flavobacterium waterii for

Method used

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  • Bacterial strain used for short-cut denitrification for nitrogen removal and its application
  • Bacterial strain used for short-cut denitrification for nitrogen removal and its application
  • Bacterial strain used for short-cut denitrification for nitrogen removal and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Enrichment culture and separation and purification of the bacteria of embodiment 1

[0027] 1. Enrichment culture of denitrifying bacteria

[0028] The activated sludge in the nitrification tank is taken, and the culture medium containing nitrite is used for enrichment and cultivation under the condition of low dissolved oxygen for a certain period of time to obtain the denitrification activated sludge. During the enrichment process, fresh culture medium was replenished in due course.

[0029] Enriched medium formula: NaNO 2 , methanol, also contains Fe 2+ , Mg 2+ 、K + , Ca 2+ four metal cations. The culture conditions are: temperature 30°C; pH 8.2, DO (dissolved oxygen) 0.2-1.0mg L -1 .

[0030] The molar configuration ratio of the four metal cations is 1:(4-8):(5-15):(1-5). During the cultivation process, a growth promoter with a total metal cation concentration of 0.15 mol / L was added every day, and the amount added was 0.1% of the volume of the material in ...

Embodiment 2

[0034] Example 2 Investigation of denitrification ability of bacterial strains of the present invention

[0035] First, pick a single colony FDN-2 from the plate and inoculate it into beef extract peptone medium at 30°C and 120r / min on a shaker for 48 hours. After the bacterial suspension was centrifuged, it was transferred to the sodium nitrite-methanol culture solution. Under the same conditions, two methods of batch water exchange and batch feeding were adopted to investigate the denitrification ability. Table 1 shows the simultaneous removal effect of bacterial strains of the present invention on nitrous nitrogen and COD under the condition of batch water change. From the data in the table, in the process of increasing the total nitrogen concentration of influent from 241mg / L to 361mg / L, the total nitrogen removal rate is always greater than 90%. Greater than 78%. It can be seen that the strain can also remove high-concentration COD while processing total nitrogen.

[0...

Embodiment 3

[0039] Example 3 Application of bacterial strains of the present invention in the denitrification process of monosodium glutamate wastewater

[0040] Get the bacterium suspension that a certain amount of embodiment 2 obtains and inoculate according to the inoculum size of 15%, at temperature 28 ℃, pH be 7.7, DO (dissolved oxygen) is 0.8mg L -1 Under certain conditions, the effluent of the short-range nitrification reaction of the ammonia-containing wastewater produced in the production process of monosodium glutamate is treated. In the water: NO 2 - - The average of N is 200mg / L, and the average of COD is 800mg / L. After treatment, the effluent NO 2 - -N and COD average concentrations were 5.7mg / L and 46mg / L, NO 2 - The average removal rates of -N and COD were 97% and 87%, respectively.

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Abstract

The invention relates to a bacterial strain used for short-cut denitrification for nitrogen removal and its application. The bacterial strain used for short-cut denitrification for nitrogen removal is flavobacterium mizutaii FDN-2, which is preserved in China general microbiological culture collection center on March 11, 2012 and has a preservation number of CGMCC NO.3659. The bacterial strain can directly utilize nitrite nitrogen as a substrate to complete a short-cut denitrification process. When treating ammonia-containing wastewater with the flavobacterium mizutaii FDN-2 of the invention,the process is simple, and the flavobacterium mizutaii FDN-2 can tolerate a high concentration organic carbon source. When the flavobacterium mizutaii FDN-2 in the invention is put into use, a systemcan be rapidly initiated, so that the flavobacterium mizutaii FDN-2 boasts very broad application prospects in various nitrogen removal processes of wastewater.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, in particular to a high-efficiency nitrous acid type (short-range denitrification) denitrification bacterial strain. The denitrification bacterial strain of the invention can carry out denitrification with nitrous nitrogen as a nitrogen source, and is especially suitable for denitrification in the process of short-range nitrification and denitrification of ammonia-containing wastewater. Background technique [0002] The traditional biological denitrification process mainly includes two processes of nitrification and denitrification. Nitrification is the premise of the denitrification process, and the denitrification process can truly remove nitrogen-containing compounds in wastewater. Because nitrifying bacteria have strong aerobic properties, the nitrification process is aerobic; while denitrifying bacteria are generally facultative bacteria, which use oxygen as an electron acceptor u...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/28
Inventor 高会杰李志瑞黎元生许谦刘忠生
Owner CHINA PETROLEUM & CHEM CORP
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