Preparation method and application of immobilized bacteria for improving denitrification efficiency of constructed wetland

A technology of artificial wetland and denitrification efficiency, applied in chemical instruments and methods, fixed on/in organic carriers, aerobic process treatment, etc., can solve the problem of reducing cell activity, short service life, poor mass transfer performance, etc. problem, to achieve good biocompatibility, long service life and good stability
CN102392011AInactive Publication Date: 2012-03-28INST OF AQUATIC LIFE ACAD SINICA
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF AQUATIC LIFE ACAD SINICA
Publication Date
2012-03-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method and an application of immobilized bacteria for improving the denitrification efficiency of a constructed wetland. 1. The preparation steps are as follows: 1) screening and culturing aerobic denitrification strains which are screened out of the constructed wetland in a thermotank; 2) selecting aerobic denitrification bacteria in slope conservation, inoculating into a sterile enrichment culture medium and aerating to get bacterial suspension; 3) using sodium alginate-polyvinyl alcohol to embed so as to get the immobilized aerobic denitrification bacteria; and 4) taking pellets of the aerobic denitrification bacteria out of a refrigerator, washing with double distilled water or physiological saline, soaking in the physiological saline, and aerating to activate the immobilized pellets. 2. An application of the immobilized bacteria in the constructed wetland is as follows: filling the immobilized pellets into a simulation column of the constructed wetland for performing waste water denitrification treatment. The preparation method of the immobilized bacteria is simple, the production cost is low, the application in the industrial waste water treatment is easy, the denitrification efficiency is high, the accumulation amount of nitrite nitrogen is low, and the service life is long.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and more specifically relates to a method for preparing immobilized bacteria for improving nitrogen removal efficiency in constructed wetlands and an application of prepared immobilized microspheres in constructed wetlands. Background technique

[0002] With the rapid development of industry and agriculture, the discharge of industrial "three wastes" has increased, domestic sewage, medical sewage, domestic garbage, and large-scale use of chemical fertilizers and pesticides in agriculture have caused water resources to be polluted to varying degrees, among which nitrate pollution is the main pollution type of water resources one. In recent years, constructed wetlands have played an important role in sewage treatment. Substrate, higher plants, and microorganisms are the three main factors for the purification of constructed wetlands. Many studies have shown that the primary factor for nitrogen remova...

Claims

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