Kocuria palustris strain and applications thereof

A technology of coxa swampis and strains, which is applied in the field of environmental microorganisms, can solve the problems such as the coxa swampis that have not yet been seen, and achieves the effects of saving carbon sources, high denitrification activity and simple process

Active Publication Date: 2013-05-15
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of Corkia palustris in treating industrial wastewater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Enrichment culture and isolation and purification of bacteria

[0022] 1. Enrichment culture of denitrifying bacteria

[0023] The activated sludge in the nitrification tank of the sewage treatment plant is taken, and the medium containing nitrite is used for enrichment and cultivation under low dissolved oxygen conditions for a certain period of time to obtain denitrification activated sludge. During the enrichment process, fresh culture medium was replenished in due course.

[0024] Enriched medium formula: NaNO 2 , methanol, also contains Fe 2＋ , Mg 2＋ 、K ＋ , Ca 2＋ four metal cations. The culture conditions are: temperature 30°C; pH 8.2, DO (dissolved oxygen) 0.2-1.0 mg·L –1 .

[0025] The molar configuration ratio of the four metal cations is 1:(4-8):(5-15):(1-5). During the cultivation process, a growth promoter with a total metal cation concentration of 0.15 mol / L was added every day, and the added amount was 0.1% of the volume of the material i...

Embodiment 2

[0029] Example 2 Investigation of denitrification ability of bacterial strains of the present invention

[0030]First, a single colony FSDN-A was picked from the plate and inoculated into beef extract peptone medium at 30°C and 120r / min on a shaker for 42 hours. After the bacterial suspension was centrifuged, it was transferred to the sodium nitrite culture solution. Under the same conditions, the denitrification ability was investigated by batch feeding, and the lubricating oil wastewater was used as the carbon source. The influent nitrite nitrogen concentration is 150~400mg / L, the total nitrogen concentration is 200~780mg / L, and the COD concentration is 300~800mg / L. In the process of feeding in batches and gradually increasing the concentration, the reaction was carried out for 24 hours, and the removal rate of nitrite nitrogen could reach up to 26.17 mg / (L·h), and the removal rate of total nitrogen and COD could reach more than 90%. It can be seen that the strain has a hig...

Embodiment 3

[0031] Example 3 Application of the bacterial strain of the present invention in catalyst wastewater denitrification process

[0032] Take a certain amount of bacterial suspension obtained in Example 2 and inoculate according to the inoculum size of 15% (volume). – 1 Under certain conditions, denitrification treatment is carried out on the short-cut nitrification effluent of ammonia-containing wastewater generated in the catalyst production process. The COD in the water is 800 mg / L ~1200mg / L, 2 - -N is 500 mg / L ~780mg / L, NO 3 - -N is 10 mg / L. After treatment, the total nitrogen removal rate in the wastewater is over 95%, and the COD removal rate is over 90%.

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PUM

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Abstract

The invention relates to a Kocuria palustris strain and applications thereof. The strain is Kocuria palustris FSDN-A, and is preserved in the China General Microbiological Culture Collection Center on July 14, 2011, wherein a preservation number is CGMCCNO.5061. According to the present invention, the strain can adopt nitrite nitrogen or nitrate nitrogen as a substrate to complete a denitrification process; when the Kocuria palustris FSDN-A is adopted to treat ammonia-containing wastewater, characteristics of simple process, high denitrogenation activity, and high concentration organic carbon source tolerance are provided; after the strain is poured, the system is rapidly started; and the strain has broad application prospects in wastewater denitrogenation treatment processes, and is especially suitable for treatment of wastewater containing nitrogen oxides and organic pollutants.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a denitrification bacterial strain and application thereof. The denitrification bacterial strain of the present invention can carry out denitrification with nitrous nitrogen as a nitrogen source, and can also remove COD at the same time, and is especially suitable for denitrification in the process of short-range nitrification and denitrification of ammonia-containing wastewater. Background technique [0002] The traditional biological denitrification process mainly includes two processes of nitrification and denitrification. Nitrification is the premise of the denitrification process, and the denitrification process can truly remove nitrogen-containing compounds in wastewater. Because nitrifying bacteria have strong aerobic properties, the nitrification process is aerobic; while denitrifying bacteria are generally facultative bacteria, which use oxygen as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 高会杰张鹏孙丹凤李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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