Pseudomonas aeruginosa and application of pseudomonas aeruginosa in aspect of degrading aflatoxin
A technology of Pseudomonas aeruginosa and aflatoxin, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of complex degradation products, unstable effects, and large loss of nutrients, and achieve good results The effect of applying the foreground
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Embodiment 1
[0031] Embodiment 1, the isolation and identification of bacteria
[0032] 1. Isolation of bacteria
[0033] (1) In June 2013, the collected soil samples were shaken in sterile distilled water for 15 minutes in an ultra-clean workbench to prepare a bacterial suspension, and the shaker speed was 180rpm.
[0034] (2) Gradiently dilute the bacterial suspension with sterile distilled water and spread it on the NA medium plate, culture it at 30°C for 24 hours, the colonies cover the whole plate, pick the shape, size and color of the plate with an inoculation loop Strains with different transparency were purified by streaking on the plate, and the purified strains were applied to the aflatoxin degradation experiment. The obtained strain with the strongest aflatoxin degradation ability was named N17-1.
[0035] 2. Identification
[0036] (1) According to the method described in "Berger's Bacteria Identification Handbook" (Eighth Edition) and "Common Bacteria System Identification H...
Embodiment 2
[0048] Embodiment 2, the cultivation of Pseudomonas aeruginosa N17-1
[0049] Pseudomonas aeruginosa (Pseudomonas aeruginosa) N17-1 (CGMCC No.8511) was inoculated in liquid NB medium, shaken and cultured for 24 hours at a temperature of 37 °C and a rotation speed of 200 rpm (rotation radius 20 mm) to obtain bacteria solution for aflatoxin degradation experiments.
Embodiment 3
[0050] Embodiment 3, Pseudomonas aeruginosa N17-1 to aflatoxin B 1 Degradation
[0051] 1. Aflatoxin B 1 preparation
[0052] 1 mg aflatoxin B 1 (AFB 1 ) Standards were dissolved in 2ml of chromatographically pure methanol to obtain aflatoxin B with a concentration of 500ppb 1 the solution
[0053] Two, get the bacterium liquid that 0.4ml embodiment 2 obtains and place in the 1.5ml centrifuge tube, add the aflatoxin B that 0.1ml concentration is 500ppb 1 To a final concentration of 100 ppb, mix well and let stand at 37°C for 72 hours, then centrifuge at 10,000g for 10 minutes to remove cells, and obtain supernatant, which is recorded as the solution of the experimental group. Add 0.1ml of aflatoxin B with a concentration of 500ppb to 0.4ml of NB medium without bacteria 1 As a control group, it was recorded as a control group solution.
[0054] 3. N17-1 to aflatoxin B 1 Degradation effect detection
[0055] First, 100% methanol was added to the experimental group solu...
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