A strain of Pseudomonas aeruginosa and its application in removing heavy metal ions

A technology based on Pseudomonas aeruginosa and heavy metal ions, which is applied in the direction of bacteria, microbial-based methods, and the restoration of contaminated soil, etc., can solve the problems of poor ability to remove heavy metals, few heavy metal-resistant populations, and large impacts

Inactive Publication Date: 2016-04-13
曾泽华 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the past ten years, the research on heavy metal-resistant microorganisms in my country has mainly focused on the properties of microorganisms and the influence of heavy metals on bacterial cells, while there are few studies on strain screening, strain characteristics, and growth conditions.
At present, there are few isolated heavy metal-resistant populations, the growth and reproduction of bacteria are greatly affected by the habitat environment, and the ability to remove heavy metals is not strong, and it cannot meet the complex pollution status of the external actual environment. ability to remove

Method used

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  • A strain of Pseudomonas aeruginosa and its application in removing heavy metal ions
  • A strain of Pseudomonas aeruginosa and its application in removing heavy metal ions
  • A strain of Pseudomonas aeruginosa and its application in removing heavy metal ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0111] Removal of heavy metal ions Zn 2+ strain screening

[0112] 1 Experimental materials

[0113] 1.1 Experimental strains

[0114] Sample source

[0115] Soil or water samples from different locations were collected from Weifang, Rizhao, Nanjing and other areas. The soil samples were collected from 1-4cm topsoil in multiple places in the sampling area and mixed separately. There are 27 samples in total.

[0116] 1.2 Experimental Instruments

[0117] Vertical pressure steam sterilizer (HirayamaHVE-50, Japan); air bath shaker (IKAKS4000ic, Germany); analytical balance (MettlerToledoPL602-S, Switzerland); constant temperature incubator (MMMIncucell111, Germany); pipette gun (Eppendorf, Germany); Centrifuge (Eppendorf 5417R, Germany); Inductively Coupled Plasma Mass Spectrometry (ICP-MS, Agilent 7700X, USA), etc.

[0118] 1.3 Experimental reagents

[0119] Zinc sulfate [ZnSO 4 ×7H 2 O] Chemical reagents were of analytical grade. Calculate the mass fraction of metal i...

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Abstract

The present invention discloses a strain of Pseudomonas aeruginosa ZC6, which provides a heavy metal ion Zn<2+> removing effect. The method for screening Pseudomonas aeruginosa providing a heavy metal ion Zn<2+> removing effect from soil and water comprises: a, preparing a metal selective culture medium; b, carrying out enrichment acclimatization on a heavy metal resistance strain; c, screening and re-screening the heavy metal resistance strain; and d, detecting the heavy metal removing capability of the strain. According to the present invention, with the detection test on the heavy metal removing capability of the strain obtained by carrying out enrichment acclimatization on the heavy metal resistance strain with the metal ion-containing culture medium and purification, the reasonable and effective screening method for the heavy metal removing strain is established so as to obtain the strain suitable for requirements; and with application of the Pseudomonas aeruginosa ZC6 in the heavy metal pollution treatment, advantages of no secondary pollution, high treatment efficiency, wide application range, low cost and the like are provided.

Description

technical field [0001] The invention relates to a strain of microorganism and its application in removing heavy metal ions, in particular to a strain of Pseudomonas aeruginosa and its application in removing heavy metal pollution, belonging to the technical field of detection and application of microorganisms. Background technique [0002] Heavy metals are widely distributed in the atmosphere, biosphere, lithosphere, and hydrosphere. After being discharged into the environment, they are permanent and have obvious cumulative effects. They can pollute water bodies and soils and eventually accumulate in organisms through food chain cycles, resulting in serious Harm the ecological environment and human health. [0003] The technology of microbial treatment of heavy metal pollution is an emerging field that is constantly being explored and developed in the world. The use of biosorbents prepared by microbial systems to treat heavy metal pollution is currently the most promising me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/385C02F101/20
Inventor 张伟赵晗王亮亮郭礼强
Owner 曾泽华
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