A strain of Pseudomonas aeruginosa and its application in removing heavy metal ions
A technology based on Pseudomonas aeruginosa and heavy metal ions, which is applied in the direction of bacteria, microbial-based methods, and the restoration of contaminated soil, etc., can solve the problems of poor ability to remove heavy metals, few heavy metal-resistant populations, and large impacts
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[0111] Removal of heavy metal ions Zn 2+ strain screening
[0112] 1 Experimental materials
[0113] 1.1 Experimental strains
[0114] Sample source
[0115] Soil or water samples from different locations were collected from Weifang, Rizhao, Nanjing and other areas. The soil samples were collected from 1-4cm topsoil in multiple places in the sampling area and mixed separately. There are 27 samples in total.
[0116] 1.2 Experimental Instruments
[0117] Vertical pressure steam sterilizer (HirayamaHVE-50, Japan); air bath shaker (IKAKS4000ic, Germany); analytical balance (MettlerToledoPL602-S, Switzerland); constant temperature incubator (MMMIncucell111, Germany); pipette gun (Eppendorf, Germany); Centrifuge (Eppendorf 5417R, Germany); Inductively Coupled Plasma Mass Spectrometry (ICP-MS, Agilent 7700X, USA), etc.
[0118] 1.3 Experimental reagents
[0119] Zinc sulfate [ZnSO 4 ×7H 2 O] Chemical reagents were of analytical grade. Calculate the mass fraction of metal i...
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