Method for killing pseudomonas aeruginosa

A Pseudomonas aeruginosa, low-temperature plasma technology, applied in sanitary equipment, plasma, electrical components and other directions for toilets, can solve problems such as difficult to kill, difficult clinical treatment, etc., achieve pollution-free killing, low temperature , to avoid the effect of degradation and denaturation

Inactive Publication Date: 2013-04-17
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The bacterium has strong environmental adaptability and resistance to antibacterial drugs, and often manifests as multidrug resistance or even all resistance to antipseudomonal drugs commonly used in clinical practice. Once infected, clinical treatment is very difficult
[0004] Pseudomonas aeruginosa has strong drug resistance and is not easy to be killed by drugs; moreover, the bacterium has strong resistance to the external environment and can survive for a long time in humid places. to kill

Method used

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  • Method for killing pseudomonas aeruginosa

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0018] First, draw 10 μl of freshly prepared Pseudomonas aeruginosa suspension and evenly spread it on the treated PET slide, after drying, place it in the discharge reaction tube of the radio frequency plasma discharge device. PET is polyethylene terephthalate (polyethylene terephthalate), also known as polyester resin, linear polyester; foreign trade names Dacron, Tetoron, Terylene, Lavsan, is a common medical polymer material.

[0019] Then, adjust the discharge parameters of the radio frequency plasma discharge device as follows: the working gas is oxygen, the discharge power is 40W, and the gas flow rate is 20cm 3 / min, voltage 440V, current 105mA, background vacuum 7.0Pa; the sterilization effect after 30s is 4.18, and the sterilization rate is 99.99%; it tends to be stable after 80-90s, and the sterilization reaction is close to complete after 90s; at this time, The plasma temperature was the highest at 23°C. Wherein, the sterilization effect is calculated by the follo...

experiment example 2

[0021] First, draw 10 μl of freshly prepared Pseudomonas aeruginosa suspension and evenly spread it on the treated PET slide, after drying, place it in the discharge reaction tube of the radio frequency plasma discharge device.

[0022] Then, adjust the discharge parameters of the radio frequency plasma discharge device as follows: the working gas is oxygen, the discharge power is 40W, and the gas flow rate is 20cm 3 / min, voltage 440V, current 105mA, background vacuum 7.0Pa; the sterilization effect after 60s is 5.46, and the sterilization rate is 99.99%; it tends to be stable after 80-90s, and the sterilization reaction is close to complete after 90s; at this time, The plasma temperature was the highest at 23°C.

[0023] This experimental example shows that low-temperature air plasma can effectively kill Pseudomonas aeruginosa in a short time.

experiment example 3

[0025] First, draw 10 μl of freshly prepared Pseudomonas aeruginosa suspension and evenly spread it on the treated PET slide, after drying, place it in the discharge reaction tube of the radio frequency plasma discharge device.

[0026] Then, adjust the discharge parameters of the radio frequency plasma discharge device as follows: the working gas is oxygen, the discharge power is 40W, and the gas flow rate is 20cm 3 / min, voltage 440V, current 105mA, background vacuum 7.0Pa; after 90s, the sterilization effect is 5.52, and the sterilization rate is 99.99%; after 80-90s, it tends to be stable, and after 90s, the sterilization reaction is close to complete; at this time, The plasma temperature was the highest at 23°C.

[0027] Experimental examples 1-3 illustrate that low-temperature oxygen plasma can effectively kill Pseudomonas aeruginosa in a short time.

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Abstract

The invention relates to the field of disinfection and sterilization, which discloses a method for killing pseudomonas aeruginosa. The method is achieved only by putting a carrier infected with the pseudomonas aeruginosa in plasma environment at a low temperature of between 10 DEG C and 50 DEG C for killing the pseudomonas aeruginosa for 30-100 seconds, wherein the low-temperature plasma environment is generated by a radio frequency plasma discharge device.

Description

technical field [0001] The invention relates to the field of disinfection and sterilization, in particular to a method for killing Pseudomonas aeruginosa. Background technique [0002] Pseudomonas aeruginosa (Pseudomonas aeruginosa), formerly known as Pseudomonas aeruginosa, is widely distributed in nature. It is one of the most common bacteria in soil and one of the main pathogens of hospital infection. The important condition for its existence is moist environment. All kinds of water, air, skin, respiratory tract and intestinal tract of normal people have this bacteria. Patients suffering from metabolic diseases, blood diseases and malignant tumors, as well as patients after surgery or certain treatments are susceptible to infection with this bacteria. Pseudomonas aeruginosa often causes postoperative wound infection, and can also cause bedsores, abscesses, and suppurative otitis media; the infected lesions can cause hematogenous dissemination, resulting in bacteremia an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/14H05H1/30
Inventor 陈杰瑢杨利青
Owner XI AN JIAOTONG UNIV
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