Method for killing pseudomonas aeruginosa
A Pseudomonas aeruginosa, low-temperature plasma technology, applied in sanitary equipment, plasma, electrical components and other directions for toilets, can solve problems such as difficult to kill, difficult clinical treatment, etc., achieve pollution-free killing, low temperature , to avoid the effect of degradation and denaturation
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experiment example 1
[0018] First, draw 10 μl of freshly prepared Pseudomonas aeruginosa suspension and evenly spread it on the treated PET slide, after drying, place it in the discharge reaction tube of the radio frequency plasma discharge device. PET is polyethylene terephthalate (polyethylene terephthalate), also known as polyester resin, linear polyester; foreign trade names Dacron, Tetoron, Terylene, Lavsan, is a common medical polymer material.
[0019] Then, adjust the discharge parameters of the radio frequency plasma discharge device as follows: the working gas is oxygen, the discharge power is 40W, and the gas flow rate is 20cm 3 / min, voltage 440V, current 105mA, background vacuum 7.0Pa; the sterilization effect after 30s is 4.18, and the sterilization rate is 99.99%; it tends to be stable after 80-90s, and the sterilization reaction is close to complete after 90s; at this time, The plasma temperature was the highest at 23°C. Wherein, the sterilization effect is calculated by the follo...
experiment example 2
[0021] First, draw 10 μl of freshly prepared Pseudomonas aeruginosa suspension and evenly spread it on the treated PET slide, after drying, place it in the discharge reaction tube of the radio frequency plasma discharge device.
[0022] Then, adjust the discharge parameters of the radio frequency plasma discharge device as follows: the working gas is oxygen, the discharge power is 40W, and the gas flow rate is 20cm 3 / min, voltage 440V, current 105mA, background vacuum 7.0Pa; the sterilization effect after 60s is 5.46, and the sterilization rate is 99.99%; it tends to be stable after 80-90s, and the sterilization reaction is close to complete after 90s; at this time, The plasma temperature was the highest at 23°C.
[0023] This experimental example shows that low-temperature air plasma can effectively kill Pseudomonas aeruginosa in a short time.
experiment example 3
[0025] First, draw 10 μl of freshly prepared Pseudomonas aeruginosa suspension and evenly spread it on the treated PET slide, after drying, place it in the discharge reaction tube of the radio frequency plasma discharge device.
[0026] Then, adjust the discharge parameters of the radio frequency plasma discharge device as follows: the working gas is oxygen, the discharge power is 40W, and the gas flow rate is 20cm 3 / min, voltage 440V, current 105mA, background vacuum 7.0Pa; after 90s, the sterilization effect is 5.52, and the sterilization rate is 99.99%; after 80-90s, it tends to be stable, and after 90s, the sterilization reaction is close to complete; at this time, The plasma temperature was the highest at 23°C.
[0027] Experimental examples 1-3 illustrate that low-temperature oxygen plasma can effectively kill Pseudomonas aeruginosa in a short time.
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