Primer, kit and method for detecting pseudomonas aeruginosa and plcH in water

Abstract

The invention discloses a primer, a kit and a method for detecting pseudomonas aeruginosa and plcH in water, and aims to overcome the shortcomings of the prior art. The sequence of the primer and a probe sequence include an upstream primer P.AF, a downstream primer P.AR, a probe P.AP, an upstream primer plcHF, a downstream primer plcHR and a probe plcHP. The kit is reasonable in composition and proportion, convenient to use, rapid and accurate in detection and applicable to double ddPCR detection of the pseudomonas aeruginosa and plcH genes in the water. The method is used for detecting the pseudomonas aeruginosa and the plcH genes in the water by the kit, simple, convenient and rapid in detection and accurate in detection result.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

AI Technical Summary

Benefits of technology

This patented technology allows us to quickly and easily measure pseudomonal DNA (DNA that causes bacterial diseases). By measuring specific parts or regions within this DNA sequence we don't have any technical problem addressed beforehand but rather they are just starting to become important because these techniques help researchers better identify germs like those causing disease more effectively than previous methods such as cultures.

Problems solved by technology

Pneumoniae caused by pseudonema bacillus is becoming increasing problem worldwide due to their ability to cause diseases like acute lung injury disease (ALI). Current testing protocols involve measuring levels of antibiotic resistance called PlkA from different parts of the body including blood supply systems, gastrointestinals, lungs, urinary system, uteroscopics, dentritives, teeth pulp tissue culture media, and natural waters. These tests suffer limitations because they often lack sensitivity and precision, making them difficult to detect both true positive and false negative forms of pathogens. There remains room for improvement in terms of enzymatic activity assay platforms capable of accurately identifying Prionella sppansis polysiloxymerase gene variants associated with pseudoerospora bacilli.