84 results about "Marine mammal" patented technology

Described are transgenic, non-human animals comprising a nucleic acid encoding an immunoglobulin light chain, whereby the immunoglobulin light chain is human, human-like, or humanized. The nucleic acid is provided with a means that renders it resistant to DNA rearrangements and / or somatic hypermutations. In one embodiment, the nucleic acid comprises an expression cassette for the expression of a desired molecule in cells during a certain stage of development in cells developing into mature B cells. Further provided is methods for producing an immunoglobulin from the transgenic, non-human animal.

The invention provides knock-in non-human cells and mammals having a genome encoding chimeric antibodies and methods of producing knock-in cells and mammals. Certain aspects of the invention include chimeric antibodies, humanized antibodies, pharmaceutical compositions and kits. Certain aspects of the invention also relate to diagnostic and treatment methods using the antibodies of the invention.

In general, the invention features genetically modified non-human mammals (e.g., bovines and other ungulates), and methods of making these mammals. In particular, the invention features transgenic ungulates having reduced levels of endogenous IgM heavy chain and / or prion protein.

A transgenic, non-human mammalian animal is capable of expressing a heterologous gene for human or other recombinant physiologically functional fibrinogen holoprotein or individual subunit chain polypeptides thereof or a modified or fusion fibrinogen in mammary glands of the animals and secreting the expressed product into a body fluid. Methodology employing such a mammal yields recombinant physiologically functional fibrinogens, subunit chain polypeptides thereof, and modified or fusion fibrinogens.

The present invention relates to the preparation of non-human animals having chimeric livers, whereby some or substantially all of the hepatocytes present are human hepatocytes. It is based, at least in part, on the discovery that rats, tolerized in utero against human hepatocytes, were found to serve as long-term hosts for human hepatocytes introduced post-natally, and the introduced hepatocytes maintained their differentiated phenotype, as evidenced by continued production of human albumin.

The present invention relates to methods for engineering VH domains to improve their solubility and stability. The invention provides for the incorporation of defined amino acid substitutions based on 3-D structural information into the V segments of a heavy chain locus, expressing the locus in a non-human mammal and selecting soluble VH domains. Further stabilising or solubilising mutations maybe introduced as a result affinity maturation during B-cell maturation in vivo.

This document provides methods and materials related to assessing prostate cancer in mammals. For example, this document provides nucleic acids and polypeptides that can be analyzed to determine whether a male mammal having prostate cancer is susceptible to a good or poor outcome.

Low probability of marine impact (LPMMI) sound signals have a modulation component selected to reduce a behavioral response from a marine mammal from that which would occur when transmitting another sound signal having approximately the same time duration component, approximately the same bandwidth component, and approximately the same center frequency component as the LPMMI waveform but having a modulation component comprising a frequency modulation.

The present invention belongs to the field of garden technology, and is especially method of constructing biological passage way for small wild mammal to migrate in subtropical city. The biological passage way is one 15-20 m wide green belt in symmetrical structure with dense outside and sparse inside. It consists of two marginal belts of dense complex mixed forest in 5-6 m width, one middle core belt of sparse small tree in 1-2 width, and two buffering belts between each of the marginal belts and the core belt of sparse shrub in 2-3 width. Constructing biological passage way for small wild mammal to migrate in subtropical city can raise the biodiversity and promote ecological balance in city.

A system for passive acoustic monitoring in connection with seismic surveying includes a survey vessel having a recording system thereon. At least one seismic energy source is coupled to the vessel by a first towing cable. The towing cable includes at least one conductor therein. At least one seismic sensor streamer is coupled to the vessel by a lead in cable. At least one acoustic sensor is coupled to the at least one source by a second towing cable. The second towing cable includes at least one signal conductor configured to transmit signals from the acoustic sensor to the at least one conductor in the first towing cable. The at least one acoustic sensor is configured to detect marine mammal vocalization.

The invention relates to a halobios specimen making technique, concretely a method for making specimens of large marine mammals and fishes, peeling off skin of a sample between corium layer and subcutaneous fat layer, soaking with alcohol and processing with sodium carbonate, processing with arsenic trioxide, soap and camphor, and processing the seam with unsaturated polyester putty. And it overcomes oil overflowing, and keeps original appearance of the sample, having very high economic and social benefits.

The disclosed embodiments provide an acoustic deterrent system and a method for repelling sea mammals from a region of water. The system consists of one or more acoustic impulse sources spatially distributed underwater around the perimeter of the fish farm or other region to be protected. Each source is capable of generating an acoustic signal and a controller determines the firing time for each source to produce signals, which may include precisely timed wave trains of superimposed tones on a broadband low frequency signal, which are believed most effective in deterring seals, sea lions and other carnivorous mammals. The system may further include a sensing device to determine the presence of marine mammals so that the acoustic deterrent can be initiated on demand.

Disclosed herein is a system of acquiring seismic date in a marine environment, which includes: seismic streamers towed by a vessel; and means for detecting and / or locating marine mammals, characterized in that said marine mammal detection and / or location means are secured to said seismic streamers.

The invention relates to technologies for manufacturing large marine mammal skinned specimens and discloses a novel method for degreasing hides of large marine mammals, such as whales. The method comprises the steps of soaking, degreasing, liming, deliming, softening, pickling, tanning and retanning. According to the method, a degreasing operation runs through a whole hide tanning process; and adipose tissues can be removed from the hides greatly, a tanning agent is used for replacing soluble protein in skin and undertakes a medium of a connection structure, the stability of a collagen structure is improved, and the shrinkage temperature and hydrothermal resisting stability are improved, so that the aims of no oil spill corruption, no contraction distortion, long-term storage and healthy exhibition are achieved.

A method for localizing a marine animal in an underwater environment implemented by a passive acoustic monitoring (PAM) system. The passive acoustic monitoring system includes network of acoustic sensors adapted to carry out measurements of acoustic signals. The method includes: obtaining, from the network of acoustic sensors, measured data of an acoustic signal emitted by a marine animal; defining a space of dimensional representation of the underwater environment discretized in a plurality of predefined cells, for each cell of a predefined set, obtaining an error defined as a value of a cost function for the cell, the cost function giving a deviation between: at least some of the measured data; and corresponding theoretical data obtained by assuming the marine animal is located in the cell, the errors obtained for the predefined set of cells enabling to obtain at least one piece of information about localization of the marine animal.

The invention relates to the field of manufacturing a gene knockout animal model, in particular to a method of preparing an alpha-1,3GT gene knockout non-human mammal and an application. The preparation method comprises the steps of separating an alpha-1,3GT gene from genomic DNA (Deoxyribose Nucleic Acid), carrying out amplification via a long-chain PCR (Polymerase Chain Reaction) method to obtain a homologous arm, compositing with an antibiotic drug resistance gene to construct a targeting vector, transferring the targeting vector into an embryonic cell, injecting the recombined embryonic cell into an embryo of a surrogacy animal, transplanting into a pseudopregnancy animal body for mating with a normal animal, carrying out genotype verification on an obtained chimera animal, screening a positive gene knockout chimera animal with successful gene knockout for further mating with a wild animal to obtain F1 generation heterozygotes, obtaining a homozygote animal with two chromosomes removed after mating of the F1 generation heterozygotes, and establishing a line to obtain a gene knockout animal population.

The invention relates to the field of preparing gene knockout animal models, and particularly relates to a method for preparing a non-human mammal with iGb3S gene knockout and applications. The method includes: separating an iGb3S gene from genome DNA, amplifying through a long-chain RCR method to obtain a homologous arm, compositing with an antibiotic resistance gene to construct a targeting vector, transferring the targeting vector into an embryonic cell, injecting the recombinant embryonic cell into a surrogate animal embryo, transplanting the embryo into a pseudopregnancy animal, mating with an normal animal, subjecting the obtained chimera animals to genotype verification, screening the chimera animal with positive gene knockout and success gene knockout, further mating with wild type animals to obtain F1 heterozygotes, mating the F1 heterozygotes to obtain a homozygote animal with both two chromosomes being deleted, and establishing to obtain a gene knockout animal population.

This invention provides a system apparatus and method for ship-towed deployment of a non-linear volumetric array of hydrophones, allowing line-intersect or line-transect sampling of marine mammal populations through passive acoustic monitoring, enabling unambiguous real-time three-dimensional localization of single sounds received through a low-cost, modular, robust, stable, small, light, neutrally to slightly negatively buoyant volumetric array having low self-noise and low flow noise, that avoids putting high tension on the tow cable and that is compatible with standard hydrophones, instrumentation, cabling, and analytical software.

An energy-harvesting compute grid includes computing assemblies that cooperate with mobile energy harvesters configured to be deployed on a body of water. The plurality of energy harvesters are positioned on and move adjacent to an upper surface of a body of water, and the locations of the energy harvesters can be monitored and controlled. The wide-spread gathering by the harvesters of environmental data within that geospatial area permits the forecasting of environmental factors, the discovery of advantageous energy-harvesting opportunities, the observation and tracking of hazardous objects and conditions, the efficient distribution of data and / or tasks to and between the harvesters included in the compute grid, the efficient execution of logistical operations to support, upgrade, maintain, and repair the cluster, and the opportunity to execute data-gathering across an area much larger than that afforded by an individual harvester (e.g., radio astronomy, 3D tracking of and recording of the communication patterns of marine mammals, etc.). The computational tasks can be shared and distributed among a compute grid implemented in part by a collection of individual floating self-propelled energy harvesters thereby providing many benefits related to cost and efficiency that are unavailable to relatively isolated energy harvesters, and likewise unavailable to terrestrial compute grids of the prior art.

The present invention is directed to a method of assessing in vivo human glial cell response to pathogenic infection that involves providing a non-human mammal either with at least 30% of its glial cells in its corpus callosum being human glial cells and / or with at least 5% of its glial cells its brain and brain stem white matter being human glial cells, subjecting the non-human mammal to pathogenic infection and assessing the in vivo human glial cell response to pathogenic infection. A method of identifying therapeutic agents for the pathogenic infection as well as forms of the non-human mammal having a pathogenic brain infection are also disclosed.

The invention discloses a medicinal injection for killing coral predator acanthaster planci. The medicinal injection for killing the coral predator acanthaster planci contains Vibrio alginolyticus, Vibrio parahaemolyticus and a culture medium. The Vibrio alginolyticus HN492 and the Vibrio parahaemolyticus A16 in the medicinal injection for killing the acanthaster planci are separated from a seawater environment, are harmless to the environment under conventional concentrations, do not belong to pathogenic strains, and do not have pathogenicity for marine animals of marine fishes, shrimps, acanthaster planci and the like. According to the mechanism of the medicinal injection of killing the acanthaster planci, the bacteria form ultrahigh concentrations in acanthaster planci bodies locally, various enzymes and metabolites which are secreted by the bacteria cause damage to tissues in the acanthaster planci bodies, and a large amount of bacterial protein causes stress for the acanthaster planci, and causes death at last.

Compositions and methods useful for fishing are described. In particular, compositions which include one or more fish attractants and which combine several advantageous properties, including moldability at ambient temperatures, sustained release of attractant(s), and ability to resist casting forces due to increased wet strength, are described. These advantages are achieved by combining a polymer such as a cellulose ester, a plasticizer, one or more fish attractant food sources, and optionally one or more fillers. The compositions may include up to 50% attractants, rendering them an effective bait material for attracting fish, crustaceans, other aquatic creatures such as marine mammals, mink, beaver, nuisance pests such as insects, rodents, and coyotes or game species such as deer and bear.

The present invention discloses a method for expressing multiple recombinant proteins in transgenic non-human mammalian milk, characterized in which human clotting factor IX gene and porcine lactoferrin gene are transferred into the mammal by gene injection and embryonic implantation to obtain expression in the milk of transgenic mammal and its filial generation. The method of this invention can maintain the stable expression of multiple recombinant proteins in the transgenic mammal during lactation and stable expression amount proximate to that of the first generation in the offsprings of the transgenic non-human mammal.

The invention provides a gene diagnosing reagent box and the detecting method of sea marine lives (such as shrimp, fish and so on) and human pathogenic bacterium-vibrio alginolyticus. There agent box and the detecting method are designed using a pair of primer as main body of vibrio alginolyticus gene protected area series design. It uses poly enzyme chain reaction (PCR) technology to carry on qualitative detection to the special DNA piece of the sea marine product and human pathogenic bacterium-vibrio, the method is quick, and the singularity is excellent, the sensitivity is high; it can be applied to the bacterium tracing and detecting to the sea marine product in each period, it also can be applied to the clinic detection of human intestinal canal acute contagion, and the environment detection and so on.

This invention provides a system apparatus and method for ship-towed deployment of a non-linear volumetric array of hydrophones, allowing line-intersect or line-transect sampling of marine mammal populations through passive acoustic monitoring, enabling unambiguous real-time three-dimensional localization of single sounds received through a low-cost, modular, robust, stable, small, light, neutrally to slightly negatively buoyant volumetric array having low self-noise and low flow noise, that avoids putting high tension on the tow cable and that is compatible with standard hydrophones, instrumentation, cabling, and analytical software.

Low probability of marine mammal impact (LPMMI) sound signals have a modulation component selected to reduce a behavioral response from a marine mammal from that which would occur when transmitting another sound signal having approximately the same time duration component, approximately the same bandwidth component, and approximately the same center frequency component as the LPMMI waveform but having a modulation component comprising a frequency modulation.

The present invention discloses methods for cell nuclear transfer that comprise for example modification of zona pellucida of an oocyte, and / or sectioning of oocytes into several parts. The present invention also discloses methods for producing a genetically modified non-human mammal. Genetically modified non-human mammals obtainable by the disclosed methods are also within the scope of the present invention. Disclosed are also methods for cryopreservation of cells.