Medicinal injection for killing coral predator acanthaster planci

A starfish and medicament technology, which is applied in the field of injecting pesticides for coral predators, long-thorn starfish, can solve the problems of tissue ulceration and death, injection of medicaments, and non-pathogenicity, so as to reduce the amount of single injection and ensure safe operation Reliable, avoiding the effect of inconvenient operation

Active Publication Date: 2019-08-30
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CuSO 4 It can denature the protein in the long-thorn starfish, and eventually cause the death of the starfish, but CuSO 4 Use may cause Cu 2+ sea ​​area pollution, NaHCO 3 It can quickly increase the pH value in the body, causing the festering and death of the tissues in the body.
However, we found in practical application that CuSO 4 , NaHCO 3 When the injection dose is small, the killing effect is not obvious in actual operation; when the injection dose is large, it will cause frequent injection of injection agents, so neither of them is an ideal injection killing agent
[0005] Vibrio alginolyticus and Vibrio parahaemolyticus are very common bacterial species in the marine environment. Concentration) can cause fish and shrimp diseases in the ocean. They belong to opportunistic pathogenic bacteria, but most strains are not pathogenic. There have been no reports of pathogenicity of Vibrio alginolyticus and Vibrio parahaemolyticus on long spiny starfish.

Method used

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  • Medicinal injection for killing coral predator acanthaster planci
  • Medicinal injection for killing coral predator acanthaster planci

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Vibrio alginolyticus HN492 and Vibrio parahaemolyticus A16 were shaken and cultured overnight (cultivation time was 8 hours), the culture medium was conventional LB medium, the culture temperature was 30°C, and the rotation speed was 200rpm, thereby obtaining the bacterial liquid of Vibrio alginolyticus HN492 and the bacterial fluid of Vibrio parahaemolyticus A16. Mix the two in equal proportions (V / V) to obtain a mixed bacterial solution, and then mix the mixed bacterial solution with 5 times concentrated SKM medium at a ratio of 1:200 (V / V) to form a long-thorn starfish injection to kill The medicament is to pack the long-thorn starfish injection killing agent into the long-thorn starfish injection killing device for use.

Embodiment 2

[0032] Vibrio alginolyticus HN492 and Vibrio parahaemolyticus A16 were shaken and cultured overnight (the culture time was 14 hours), the culture medium was conventional LB medium, the culture temperature was 30°C, and the rotation speed was 200rpm, thereby obtaining the bacterial liquid of Vibrio alginolyticus HN492 and the bacterial fluid of Vibrio parahaemolyticus A16. Mix the two in equal proportions (V / V) to obtain a mixed bacterial solution, and then mix the mixed bacterial solution with 10 times concentrated SKM medium at a ratio of 1:100 (V / V) to form a long-thorn starfish injection to kill The medicament is to pack the long-thorn starfish injection killing agent into the long-thorn starfish injection killing device for use.

Embodiment 3

[0034] Vibrio alginolyticus HN492 and Vibrio parahaemolyticus A16 were shaken and cultured overnight (the culture time was 16 hours), the culture medium was conventional LB medium, the culture temperature was 30°C, and the rotation speed was 200rpm, thereby obtaining the bacterial liquid of Vibrio alginolyticus HN492 and the bacterial fluid of Vibrio parahaemolyticus A16. Mix the two in equal proportions (V / V) to obtain a mixed bacterial solution, and then mix the mixed bacterial solution with a 15-fold concentrated SKM medium at a ratio of 1:50 (V / V) to form a long-thorn starfish injection to kill The medicament is to pack the long-thorn starfish injection killing agent into the long-thorn starfish injection killing device for use.

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Abstract

The invention discloses a medicinal injection for killing coral predator acanthaster planci. The medicinal injection for killing the coral predator acanthaster planci contains Vibrio alginolyticus, Vibrio parahaemolyticus and a culture medium. The Vibrio alginolyticus HN492 and the Vibrio parahaemolyticus A16 in the medicinal injection for killing the acanthaster planci are separated from a seawater environment, are harmless to the environment under conventional concentrations, do not belong to pathogenic strains, and do not have pathogenicity for marine animals of marine fishes, shrimps, acanthaster planci and the like. According to the mechanism of the medicinal injection of killing the acanthaster planci, the bacteria form ultrahigh concentrations in acanthaster planci bodies locally, various enzymes and metabolites which are secreted by the bacteria cause damage to tissues in the acanthaster planci bodies, and a large amount of bacterial protein causes stress for the acanthaster planci, and causes death at last.

Description

technical field [0001] The invention belongs to the field of coral protection, and in particular relates to an injectable killing agent for coral predators, long-thorn starfish. [0002] Background technique: [0003] Coral is an important biological resource in tropical oceans and is of great significance to the maintenance of marine ecosystems. In recent years, affected by various factors, coral reef resources have shown a downward trend worldwide, and the protection of coral reef resources has become the consensus of tropical coastal countries. The long-thorn starfish is an echinoderm that mainly feeds on corals. The long-thorn starfish has a large appetite and strong destructive power. Its population explosion can cause a large area of ​​coral reefs to die. Therefore, the long-thorn starfish is considered to be the most destructive enemy to corals. biology. How to prevent and control long-thorn starfish has become one of the most concerned issues for coral reef managers....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P15/00
CPCA01N63/10
Inventor 罗鹏于宗赫云龙李颖颖胡超群
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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