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A kind of Pseudomonas aeruginosa bacterial strain and application thereof

A technology of Pseudomonas aeruginosa and strains, applied in the field of microorganisms, can solve the problems of mancozeb residues and other problems, and achieve the effects of fast growth, low mutation and high vitality

Inactive Publication Date: 2019-02-22
普洱学院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a Pseudomonas aeruginosa bacterial strain and its application, aiming at solving the problem of degrading mancozeb residues, providing a microbial strain for efficiently degrading mancozeb, which can efficiently degrade mancozeb Mancozeb, a microbial degradation technology that can be used to remove mancozeb residues in tobacco leaves

Method used

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  • A kind of Pseudomonas aeruginosa bacterial strain and application thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Bacterial cell culture: Use an inoculating needle to pick out the hyphae of Pseudomonas aeruginosa and inoculate them into the above-mentioned strain activation medium plate, and cultivate in an incubator at 37°C for 4 days. The colony is Non-capsular, non-spore, and mobile Gram-negative bacteria, with different shapes, arranged in pairs or short chains, are obligate aerobes. Scrape the bacterial cells with a scalpel, and inoculate them into a 500ml conical flask containing a culture solution that has been autoclaved at 121°C and 0.1MPa for 20 minutes. Then place it at 37° C., rotate at 240 rpm / min, shake culture on a shaker for 4 days, and obtain a mixture of hyphae and bacterial liquid.

Embodiment 2

[0027] Example 2 Degradation of mancozeb residues in solid medium supplemented with carbon sources: When the bacteria activation medium is sterilized and cooled to 40°C, the mancozeb pesticide that has been sterilized by ultraviolet for 30 minutes is mixed with the concentration of 5mg / L, after mixing well, pour it into a petri dish with a diameter of 60ml, 3ml medium per petri dish, the medium is pink. The hyphae cultured in Example 1 were punched into small pieces along the edge of the colony with a 6 mm diameter puncher. After the culture medium mixed with mancozeb pesticide is condensed, the small pieces of hyphae are connected, and the hyphae are pressed down against the surface of the culture medium. Placed in a 37°C incubator for 10 days, the pink color in the medium has faded, the molecular structure of mancozeb pesticide is degraded, and the medium is cream-colored and translucent.

Embodiment 3

[0028] Example 3: Degradation of high-concentration mancozeb pesticide residues by degrading bacteria

[0029] (1) Add 5 mg / L of mancozeb pesticide to 100 ml of the cultured medium, and culture at 37°C with a rotating speed of 240 rpm / min, shaking on a shaker, and add an equal concentration of generation to the uninoculated medium. Mancozeb pesticide as a control group;

[0030] (2) Sampling once every 12h, take 3ml sample and place it in 50ml of the described Pseudomonasaeruginosa (Pseudomonasaeruginosa) strain to degrade mancozeb pesticide residues: proceed as follows:

[0031] (1) Add mancozeb pesticide at a final concentration of 5-10 mg / L to 100-150 ml of the cultured medium, and culture at 30-37°C with a rotation speed of 240-260rmp / min. Add the same concentration of mancozeb pesticide to the inoculated culture solution as a control group;

[0032] (2) Sampling every 12h, take 2-3ml sample in a 5-10ml plastic centrifuge tube, add 10-12ml of acetonitrile, shake for 20-30min, cen...

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Abstract

The invention discloses a strain of Pseudomonas aeruginosa and application thereof. The preservation number of the strain of Pseudomonas aeruginosa is: CGMCC No.11776. The present invention is a strain of Pseudomonas aeruginosa that efficiently degrades the oomycete fungicide-mancozeb isolated from tobacco root soil. Experiments show that the bacterium can use mancozeb as the only carbon source in LB medium It grows with energy and degrades mancozeb; under the condition of liquid culture with exogenous nutrients, the bacteria can degrade more than 90% of the high concentration of mancozeb mixed in the medium for 48 hours, and It has a good degradation effect in a wide pH range; the bacteria can be used for the biodegradation of mancozeb residues in water and surfaces, making up for the current use of Pseudomonas aeruginosa to degrade mancozeb pesticide residues Technology gap.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and particularly relates to a Pseudomonas aeruginosa strain and its application. Background technique [0002] Chemical pesticides have the advantages of wide application range, many control objects, low production costs, good control effects and high economic benefits. Their wide application greatly promotes the development of modern agriculture, and improves the labor efficiency and mechanization of agricultural production. However, it is inevitable that a large number of pesticides are used to kill natural enemies of insects and beneficial microorganisms, make harmful organisms resistant, cause human and livestock poisoning, and easily cause phytotoxicity to plants. Some of the chemical pesticides that enter the environment can be exposed to sunlight, The effect of microorganisms in the soil is gradually degraded and invalidated; the other part will eventually enter the human body through th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/385A62D101/04A62D101/26
CPCA62D3/02A62D2101/04A62D2101/26C12N1/20C12N1/205C12R2001/385
Inventor 张春花成文章蒋智林单治国饶智王超杨应明罗华元
Owner 普洱学院
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