Pseudomonas aeruginosa nucleic acid fluorescent PCR (polymerase chain reaction) detection kit and detection method

Abstract

The invention discloses a Pseudomonas aeruginosa nucleic acid fluorescent PCR (polymerase chain reaction) detection kit and detection method. The detection kit comprises a PCR reaction solution, an enzyme mixed solution, a positive control, a negative control and an internal standard, wherein the PCR reaction solution comprises a PCR buffer solution, nucleic acid releaser, deoxyribonucleoside triphosphate, primers for target polynucleotide amplification and probes for target polynucleotide detection; the primers comprise a forward primer and a reverse primer. Or, the detection kit comprises a PCR reaction solution, an enzyme mixed solution and an internal standard, wherein the PCR reaction solution comprises a PCR buffer solution, nucleic acid releaser, deoxyribonucleoside triphosphate, a forward primer for target polynucleotide amplification, a reverse primer for target polynucleotide amplification and probes for target polynucleotide detection. The Pseudomonas aeruginosa nucleic acid fluorescent PCR detection kit shown in the embodiment of the invention with the advantages of no need of nucleic acid extraction, high detection sensitivity, wide detection range and high detection accuracy can quickly and accurately detect Pseudomonas aeruginosa DNA (deoxyribonucleic acid) in plasma, urine and other samples.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a nucleic acid fluorescence PCR detection kit and detection method of Pseudomonas aeruginosa. Background technique [0002] Pseudomonas aeruginosa is the most common Gram-negative opportunistic pathogen in nosocomial infections, which can cause acute or chronic infections such as pneumonia, meningitis, and sepsis. Because of its multi-drug resistance, as well as innate and acquired drug resistance, its clinical treatment after infection is very difficult. Therefore, the research on the detection of Pseudomonas aeruginosa DAN is particularly important. [0003] At present, the method of multi-sampling amplification and detection of Pseudomonas aeruginosa DNA in clinical practice is mainly based on real-time fluorescent quantitative PCR technology and its improvement. Real-time fluorescent quantitative PCR technology is A nucleic acid detection technology that has de...

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Problems solved by technology

[0005] The object of the invention of the present invention is to provide a kind of Pseudomonas aeruginosa nucleic acid fluorescent PCR detection kit and detection method, to solve the problem that there is a small amount of Pseudomonas aeruginosa nucleic acid detection kit developed based on real-time fluorescence quantitative PCR technology in China, the detection process, The problem of low detection accuracy

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