Genes related to Pseudomonas aeruginosa and phage infection and their application

Abstract

The invention relates to functions of genes of pseudomonas aeruginosa and an application of the genes, wherein the gene BN889_05221, the gene PA0243, the gene PA3808, the gene PA1993 and the gene PA1115 of pseudomonas aeruginosa are the necessary host genes of phage infection, and are used for screening the relevant medicines for inhibiting the phage infection. According to the invention, through the study on the relevant host genes of phage infection, a molecular mechanism for the interaction between phage and host bacteria can be well understood, therefore, a theoretical basis is provided for the treatment on the phage, meanwhile, new target genes related to virus infection can be found, and assistance is provided for screening antiviral drugs.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to the screening of specific functional genes and the development of medicinal functions, especially the genes related to Pseudomonas aeruginosa and phage infection and their application. Background technique [0002] Pseudomonas aeruginosa is one of the most common pathogens causing nosocomial infections. The current treatment method is mainly to kill or inhibit the growth of pathogenic bacteria with antibiotics. However, Pseudomonas aeruginosa itself has some drug resistance mechanisms, including the outer membrane with low permeability, the expression of constitutive genes of different efflux pumps and the production of antibiotic inactivation enzymes, and the strain can also acquire some resistance genes and reduce the expression Some porins and mutated quinolone target genes, etc., so Pseudomonas aeruginosa has developed drug resistance to many clinically used antibiotics, and the d...

AI Technical Summary

Problems solved by technology

In the bactericidal experiments of phages in vivo and in vitro, the same phage has different bactericidal efficiency against different bacteria, which is related to the fact that the process of phage infection of host bacteria is affected by the gene expression level of host bacteria. Phage lysis host depends on the mechanism of host bacteria, thereby increasing The complexity of phage lysing host bacteria

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