Genes related to Pseudomonas aeruginosa and phage infection and their application

A technology of Pseudomonas aeruginosa and phage, which is applied in the field of bioengineering, can solve the problems of different sterilization efficiencies, increasing the complexity of phage lysing host bacteria, etc.

Active Publication Date: 2019-06-11
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

In the bactericidal experiments of phages in vivo and in vitro, the same phage has different bactericidal efficiency against different bacteria, which is related to the fact that the process of phage infection of host bacteria is affected by the gene expression level of host bacteria. Phage lysis host depends on the mechanism of host bacteria, thereby increasing The complexity of phage lysing host bacteria

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  • Genes related to Pseudomonas aeruginosa and phage infection and their application
  • Genes related to Pseudomonas aeruginosa and phage infection and their application
  • Genes related to Pseudomonas aeruginosa and phage infection and their application

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[0021] The following descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention in any form; all those skilled in the art can implement the present invention smoothly according to the above description; Without departing from the scope of the technical solution of the present invention, professional technicians can use the technical content disclosed above to make some changes, modifications and equivalent changes, which are all equivalent embodiments of the present invention.

[0022] This application mainly identifies the host PAK gene related to Pseudomonas aeruginosa phage C11 infection. By constructing the transposon Tn5G mutant library of strain PAK, five mutants resistant to phage C11 were obtained. The adsorption rate of the five mutant strains was between 93.3% and 95.4%, which was not significantly different from that of the wild-type strain PAK, which was 92.7%. The five mutant genes corresponding to the...

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Abstract

The invention relates to functions of genes of pseudomonas aeruginosa and an application of the genes, wherein the gene BN889_05221, the gene PA0243, the gene PA3808, the gene PA1993 and the gene PA1115 of pseudomonas aeruginosa are the necessary host genes of phage infection, and are used for screening the relevant medicines for inhibiting the phage infection. According to the invention, through the study on the relevant host genes of phage infection, a molecular mechanism for the interaction between phage and host bacteria can be well understood, therefore, a theoretical basis is provided for the treatment on the phage, meanwhile, new target genes related to virus infection can be found, and assistance is provided for screening antiviral drugs.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to the screening of specific functional genes and the development of medicinal functions, especially the genes related to Pseudomonas aeruginosa and phage infection and their application. Background technique [0002] Pseudomonas aeruginosa is one of the most common pathogens causing nosocomial infections. The current treatment method is mainly to kill or inhibit the growth of pathogenic bacteria with antibiotics. However, Pseudomonas aeruginosa itself has some drug resistance mechanisms, including the outer membrane with low permeability, the expression of constitutive genes of different efflux pumps and the production of antibiotic inactivation enzymes, and the strain can also acquire some resistance genes and reduce the expression Some porins and mutated quinolone target genes, etc., so Pseudomonas aeruginosa has developed drug resistance to many clinically used antibiotics, and the d...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689
CPCC12Q1/689C12Q2600/136
Inventor 杨洪江崔晓莉尤甲甲
Owner TIANJIN UNIV OF SCI & TECH
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