Application of Pseudomonas aeruginosa in degrading patulin

A technology of Pseudomonas aeruginosa and patulin, applied in the field of microorganisms, can solve the problems that have not been seen in the research and reports of Pseudomonas aeruginosa degrading patulin, and achieve the effect of good application prospects

Inactive Publication Date: 2019-08-20
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The prior art discloses the application of Pseudomonas aeruginosa in the degradation of beta-cypermethrin, zearalenone, aflatoxin, ochratoxin, etc., but Pseudomonas aeruginosa has not been seen in the degradation of patulin research and reports on

Method used

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  • Application of Pseudomonas aeruginosa in degrading patulin
  • Application of Pseudomonas aeruginosa in degrading patulin
  • Application of Pseudomonas aeruginosa in degrading patulin

Examples

Experimental program
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Embodiment 1

[0024] Embodiment 1, the degradation effect of Pseudomonas aeruginosa N17-1 to patulin

[0025] NB liquid medium: composed of solvent and solute; the solute is peptone, beef extract and NaCl, the solvent is water; the concentration of peptone in NB liquid medium is 1g / 100ml, and the concentration of beef extract in NB liquid medium is 0.3 g / 100ml, the concentration of NaCl in NB liquid medium is 0.5g / 100ml.

[0026] 1. Inoculate Pseudomonas aeruginosa N17-1 in liquid NB medium to initial OD 600nm =0.05, under the condition of 37° C., shake culture at 200 rpm (rotation radius: 20 mm) for 24 hours, and collect all the bacterial liquid (that is, the entire culture system including bacterial cells and supernatant).

[0027] 2. Dissolve 1 mg of patulin standard product in 10 ml of chromatographically pure methanol to obtain a patulin solution with a concentration of 100 ppm.

[0028] 3. Prepare the experimental group solution:

[0029] After the bacterial suspension is fully mix...

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Abstract

The invention relates to the field of microorganisms, and specifically discloses a new application of pseudomonas aeruginosa, namely the new application of the pseudomonas aeruginosa to patulin degradation. According to the invention, it is proved by experiments that the pseudomonas aeruginosa can degrade the patulin effectively. The pseudomonas aeruginosa has a good application prospect in developing both of new biodegradation microbial preparations and new biodegradation sterile preparations.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the application of Pseudomonas aeruginosa in degrading patulin. Background technique [0002] Patulin, also known as patulin, is a fungal metabolite with neurotoxicity, which widely exists in a variety of food and feed processing raw materials, especially in products that use apples as processing raw materials. A large number of studies have shown that patulin has genotoxicity, cytotoxicity, immunotoxicity and reproductive toxicity, can cause DNA damage, chromosomal aberration and micronucleus formation in mammalian cells, and can damage animals' kidneys, livers and intestines and other organs organization, seriously endangering human and animal health. Along with the continuous increase in the proportion of apple juice, apple juice concentrate, cider, etc. in people's daily diet, the World Health Organization (World Health Organization, WHO) has made strict regulations on the mini...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L5/20
Inventor 刘阳赵月菊黄思琦宗英邢福国王龑
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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