Composite probiotic fermentation composition and application thereof in preparation of preparation for preventing and treating epithelial cell injury caused by mycotoxin

A technology for compounding probiotics and fermented compositions, applied in the directions of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problem of ignoring the additive and synergistic harmful effects of various mycotoxins, and alleviate epithelial cell damage. , reducing the effect of animal intestinal and health hazards

Pending Publication Date: 2020-06-16
HENAN PUAI FEED GRP CO LTD +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, people's assessment of the hazards of mycotoxins is often limited to one or a type of mycotoxins, while ignoring the additive and synergistic effects of multiple mycotoxins

Method used

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  • Composite probiotic fermentation composition and application thereof in preparation of preparation for preventing and treating epithelial cell injury caused by mycotoxin
  • Composite probiotic fermentation composition and application thereof in preparation of preparation for preventing and treating epithelial cell injury caused by mycotoxin
  • Composite probiotic fermentation composition and application thereof in preparation of preparation for preventing and treating epithelial cell injury caused by mycotoxin

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Experimental program
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Effect test

Embodiment 1

[0048] 1.1 Chemical reagents

[0049] Aflatoxin B1 (AFB1) and zearalenone (ZEA) were purchased from Sigma, USA, with a purity >99%, and were dissolved in DMSO and absolute ethanol to obtain 1 mg / mL and 10 mg / mL respectively. storage solution. According to the test design, the stock solution was diluted into different concentrations of toxin solutions with the cell culture medium DMEM / F12 containing 10% fetal bovine serum. Trypsin, PBS and MTT were all purchased from Shanghai Suo Laibao Company. The final DMSO concentration and absolute ethanol concentration in the cell culture medium were 0.1% and 0.5% (v / v), respectively.

[0050] IPEC-J2 cell line (product number C0668, purchased from Shanghai Feite Experimental Technology Co., Ltd.) is a continuous culture intestinal epithelial cell line derived from the jejunum epithelium of newborn piglets. The cells were cultured in DMEM / F12 medium containing 10% fetal bovine serum at 5×10 5 cells / mL inoculated at 25cm 2 Cell cultur...

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Abstract

The invention provides a composite probiotic fermentation composition and application thereof in preparation of a preparation for relieving epithelial cell damage caused by mycotoxin, and belongs to the technical field of mycotoxin degradation. The composite probiotic fermentation composition comprises a compound probiotic fermentation supernatant and an aflatoxin B1 degrading enzyme-zearalenone degrading enzyme compound enzyme solution, wherein the volume ratio of the compound probiotic fermentation supernatant and the aflatoxin B1 degrading enzyme-zearalenone degrading enzyme compound enzymesolution is (2.6 to 3.4) : (1.5 to 2.5). The aflatoxin B1 degrading enzyme activity of the aflatoxin B1 degrading enzyme-zearalenone degrading enzyme compound enzyme solution is 250-350 U/L, and thezearalenone degrading enzyme activity of the aflatoxin B1 degrading enzyme-zearalenone degrading enzyme compound enzyme solution is 25-35 U/L. The composite probiotic fermentation composition can effectively relieve epithelial cell damage caused by aflatoxin B1 (AFB1) and zearalenone (ZEA).

Description

technical field [0001] The invention belongs to the technical field of mycotoxin degradation, and in particular relates to a compound probiotic fermentation composition and its application in preparing preparations for preventing and treating epithelial cell damage caused by mycotoxins. Background technique [0002] Mycotoxins are ubiquitous in human food, animal feed and animal-based products, and soil. Mycotoxins are derived from Aspergillus fungi, Aspergillus and Fusarium secondary metabolites, and have high chemical stability. Currently, about 300 potentially toxic fungal metabolites produced by more than 100 species of fungi have been reported. Mycotoxins pose a hazard to human and animal food safety around the world. Among numerous mycotoxins, aflatoxin B1 (AFB1) and zearalenone (ZEA) are considered to be the main mycotoxin pollutants in feed, agricultural products and by-products. Among them, AFB1 is the most deadly, has hepatotoxicity, teratogenicity and carcinoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/43A61P17/02C12N1/20C12N1/16C12N9/00A61K35/742A61K35/747A61K36/062C12R1/245C12R1/125C12R1/72
CPCA61K35/742A61K35/747A61K36/062A61K38/43A61P17/02C12N1/20C12N1/16C12N9/00A61K2300/00
Inventor 尹清强黄玮玮常娟王平卢富山王潇宋安东刘超齐王国强耿启泉
Owner HENAN PUAI FEED GRP CO LTD
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