Aflatoxin degradation method
A technology for aflatoxin and aflatoxin, which is applied in the fields of application, food preparation, food science, etc., can solve the problems of high cost of biochemical detoxification and difficulty in preparing highly active aflatoxin detoxification active substances.
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[0012] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited to the following examples.
[0013] Instruments and reagents used in each embodiment of the present invention are as follows:
[0014] Agilent1100 liquid phase system (Agilent, USA); API3000 triple quadrupole mass spectrometry system (Applied Biosystems, USA); FW100 high-speed universal pulverizer (Tianjin Test Instrument Co., Ltd.); LD4-2 low-speed centrifuge (Beijing Medical centrifuge factory); solid phase extraction device (Supelco company in the United States); Oasis HLB solid phase extraction column (3cc / 60mg, Waters company); Methanol (chromatographically pure Fisher); Formic acid (analytical pure); n-hexane (chromatographically pure, Tianjin Damao Chemical Reagent Factory); sodium chloride; ultrapure water.
[0015] Aflatoxin B 1 Standard product 10 mg (purchased from Sigma-Aldrich, USA, purity ≥ 97%);
[0016] Aspergillus flav...
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