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A technology of aflatoxin and aflatoxin, applied in the direction of food science, etc., can solve the problems of high cost of biochemical detoxification and difficulty in preparing highly active aflatoxin detoxification active substances, so as to improve the degradation effect and improve the degradation rate effect
Inactive Publication Date: 2017-04-05
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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The cost of biochemical detoxification is relatively high, and it is difficult to pr
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Embodiment 1
[0035] A method for irradiation degradation of aflatoxin, comprising the following steps:
[0036] Step 1, inoculate Aspergillus flavus ATCC11498 (purchased from American Culture Collection Center ATCC, No. 11498 bacterial strain) on the Chapei culture medium which has been sterilized at 120°C for 15min (Chape culture medium includes 2g of sodium nitrate, dipotassium cyanide phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 15g and water 1000ml), slope, temperature 25 ℃, humidity 45%, culture for 7 days, until the slope is covered with yellow-green bacteria When the mycelium is slightly sporulated, the spore suspension is made with sterile water.
[0037] Step 2. After the peanuts are irradiated with ultraviolet light for 10 minutes to sterilize, add the peanuts to the sterile water with a mass ratio of 1:1.5 between peanuts and sterile water, and then add 2 parts by volume of the spore suspension in step 1 to mix. Evenly, a...
Embodiment 2
[0045] A method for irradiation degradation of aflatoxin, comprising the following steps:
[0046] Step 1. Inoculate Aspergillus flavus ATCC11498 (purchased from the American Culture Collection Center ATCC, No. 11498 strain) into the Chapei culture medium which has been sterilized at 125°C for 25min (Chape culture medium includes 2g of sodium nitrate, dipotassium cyanide phosphate 1g, potassium chloride 0.5g, magnesium sulfate 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g and water 1000ml), slope, temperature 30 ℃, humidity 55%, culture for 7 days, until the slope is covered with yellow-green bacteria When the mycelium is slightly sporulated, the spore suspension is made with sterile water.
[0047] Step 2. After sterilizing the peanut product under ultraviolet light for 15 minutes, add the peanut product to sterile water at a mass ratio of 1:2, and then add 3 parts by volume of spores in step 1. The suspension is mixed evenly, and cultured for 7 days at a temperature of...
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Abstract
The invention relates to an aflatoxin degradation method, in particular to an aflatoxin irradiation degradation method. The aflatoxin irradiation degradation method comprises the steps that an asporgillus flavus spore suspension is prepared; an experimental agricultural product is placed under an ultraviolet lamp to be subjected to irradiation sterilization, and the sterilized agricultural product is placed into the asporgillus flavus spore suspension to be mixed evenly and cultured, so that the experimental agricultural product infected with asporgillus flavus is obtained; the experimental agricultural product infected with the asporgillus flavus is placed into alcohol with the volume concentration being 80-85% to be soaked and stirred, and heated in a water bath with the temperature being 70-80 DEG C, and the experimental product which is infected with the asporgillus flavus and degraded preliminarily is obtained; and the experimental agricultural product infected with the asporgillus flavus is placed into a centrifugal tube to be sealed, and the sealed experimental agricultural product infected with the asporgillus flavus is placed into a <60>Co gamma ray radiation field to be irradiated and then placed into an electron beam radiation field to be irradiated. By adoption of the aflatoxin irradiation degradation method, the degradation rate of aflatoxin in the agricultural product is increased, the degrading effect is improved, industrial pollution is reduced, and cost allowance of enterprises in the production process is reduced.
Description
technical field [0001] The invention relates to a degradation method of aflatoxin. More specifically, the present invention relates to a method for irradiating and degrading aflatoxins. Background technique [0002] Aflatoxin (AF) is one of the most harmful mycotoxins to humans and animals. A large number of data confirm that aflatoxin has a strong toxic effect on the liver tissue of humans and animals, and can lead to liver cancer and even death in severe cases. Aflatoxin contamination of food is relatively serious, especially the most serious and common contamination of peanuts, walnuts, corn and their products. In addition, aflatoxins are often found in foods such as soybeans, rice, macaroni, milk and its products, and edible oils. Aflatoxin not only reduces the quality of agricultural products and feed, causing huge economic losses, but also enters the human body through animal-derived foods such as meat and milk that are contaminated or residual aflatoxin, posing a t...
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IPC IPC(8): A23L5/20
Inventor 哈益明王锋靳婧范家霖李庆鹏陈云堂郭芹崔龙
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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