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Solimonas capable of efficiently degrading aflatoxin B1 and application thereof

A kind of technology of aflatoxin and monas, which is applied in the field of microbiology and biodegradation, can solve the problems of no substantive detoxification significance and rare reports, and achieve the effect of efficient degradation and detoxification ability

Active Publication Date: 2016-01-20
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the virus-free strains reported so far have the following two common problems in practical application: First, the detoxification mechanism of some virus-free strains belongs to physical adsorption, not substantial biodegradation, and is adsorbed on the cell wall of the bacteria detoxification may occur under different physical and chemical environments in animals or humans, and has no substantial detoxification significance; second, the detoxification rate of the reported strains to toxins is mostly measured at a higher concentration of 100 μg / kg or more. There are few reports on the actual detoxification ability of low-concentration aspergillus toxoids (less than 20 μg / kg) in complex matrices such as food raw materials and feedstuffs

Method used

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  • Solimonas capable of efficiently degrading aflatoxin B1 and application thereof
  • Solimonas capable of efficiently degrading aflatoxin B1 and application thereof
  • Solimonas capable of efficiently degrading aflatoxin B1 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Screening, identification and cultivation of embodiment 1 aflatoxin B1 degrading bacteria

[0022] 1. Screening of bacterial strains

[0023] The samples were collected from soil heavily polluted by PAHs (near oil refineries, auto repair shops) and moldy food heavily polluted by mycotoxins. Take 0.5 g of the collected sample and add it to 150 mL of enrichment medium (AFB1 content is about 20 μg / L), and enrich and culture it in a constant temperature incubator at 30°C for 7 days. After the first enrichment culture, 5 mL of enrichment culture solution was inoculated into 150 mL of fresh AFB1 enrichment medium, the concentration of AFB1 was increased to 30 μg / L, and enrichment culture was continued for 7 days under the same conditions. Complete the third enrichment culture experiment with the same enrichment method, increasing the concentration of AFB1 to 50μg / L. After the enrichment culture was completed, the enriched culture solution was serially diluted with sterile w...

Embodiment 2

[0033] Example 2 Degradation characteristics of bacterial strain CW980 detected by high performance liquid chromatography

[0034] Degradation dynamic detection of degrading strains: The degrading strain CW980 was continuously activated for 2 generations on the most suitable solid medium, inoculated in 4mL liquid medium for overnight culture, and fresh bacterial liquid was obtained. 50 μL of fresh bacterial liquid was inoculated into 4 mL of AFB1 test medium (containing 20.0 μg / LAFB1), and the inoculated test tubes were shaken for 0 h, 12 h, 24 h and 48 h, and each degradation experiment was set in triplicate. Escherichia coli K12 (E.coliK12) was used as a negative control strain. After the culture was completed, mix well, centrifuge at 8000r / min for 10min, and collect the supernatant and bacterial precipitate respectively.

[0035] The degraded supernatant was passed through the AFB1 immunoaffinity column, eluted with methanol, and the eluate was collected in a glass sample ...

Embodiment 3

[0040] Example 3 Application of bacterial strain CW980 in detoxification treatment of corn soybean meal type feed

[0041] 1. Experimental materials

[0042] Strain activation medium Ⅰ: tryptone 17.0g / L, soytone 3.0g / L, glucose 2.5g / L, NaCl 5.0g / L, K 2 HPO 4 2.5g / L, agar 20.0g / L.

[0043] Strain activation medium II: peptone 5.0g / L, beef extract 30.0g / L, NaCl 5.0g / L, pH7.0-7.2.

[0044] The above-mentioned medium was autoclaved at 120°C for 15 minutes, and the experimental feed was a corn-soybean meal-based diet.

[0045] 2. Experimental method

[0046] Add an appropriate amount of AFB1 standard stock solution to 50mL of phosphate buffer solution, mix well and immediately pour into 100g of crushed feed samples, stir evenly, so that the final concentration of AFB1 reaches 40.0μg / kg respectively, and dry the feed in a cool and ventilated place Dry and set aside. Continuously activate the strains to be tested for 2 generations on the solid activation medium I, inoculate the...

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Abstract

The invention discloses solimonas capable of efficiently degrading aflatoxin B1 and application thereof, and particularly provides a solimonas (solimonas sp.) strain CW980 and application thereof in degradation of aflatoxin B1 with the low pollution concentration. Compared with an existing aflatoxin degradation bacterium, the strain CW980 can achieve the excellent degradation effect under the low-concentration aflatoxin pollution condition. Under the liquid fermentation condition, in a fermentation culture solution containing the aflatoxin B1 with the final concentration of 20 mug / L, the degradation rate of the strain CW980 to the aflatoxin B1 after 24 h is 37.8%, the degradation rate after 48 h reaches 70.3%, and the degradation rate after 72 h reaches up to 90.4 %. By processing feed polluted by the aflatoxin B1(the final concentration is 20 mug / kg) with the strain CW980 for 48 h, the AFB1 degradation rate is 23.0%, and the AFB1 degradation rate reaches 75.5% after 72 h. The strain CW980 has the substantial application value and great significance in the aspect of biological detoxification application of food and feed.

Description

technical field [0001] The invention relates to the field of microbiology and biodegradation, in particular to an Agromonas that efficiently degrades aflatoxin B1 and its application. Background technique [0002] Aspergillus mycotoxins are mainly secondary metabolites produced by Aspergillus flavus, A. (OTA) is the most toxic and most widely polluted in agricultural production and food industry. In 1993, aflatoxin was classified as a Class I carcinogen by the World Health Organization (WHO) Cancer Research Institute. Because of its strong carcinogenic, teratogenic and mutagenic properties, aflatoxin has gradually become the focus of public health and scientific research. Contamination of grain and oil crops with aflatoxins and ochratoxins has become a global problem. In animal husbandry, the two types of aspergillus can endanger animal health by contaminating feed, leading to low productivity in animal husbandry, causing serious economic losses, and through direct Or indi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L5/20A23K10/18C12R1/01
Inventor 王旭周育姜楠张正竹
Owner ANHUI AGRICULTURAL UNIVERSITY
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