Lysobacter capable of efficiently degrading aflatoxin B1 and ochratoxin A and application of Lysobacter

Abstract

The invention provides Lysobacter capable of efficiently degrading aflatoxin B1 and ochratoxin A and application of the Lysobacter and particularly provides double-function Lysobacter sp. CW239 and application thereof to the degradation of low-pollution concentration aflatoxin B1 and ochratoxin A. Compared with existing aflatoxin degrading bacteria, the Lysobacter sp. CW239 has the advantages that the Lysobacter sp. CW239 can achieve excellent degrading effect under a low-concentration toxin pollution condition; under a liquid fermentation condition, in fermentation broth, which contains the aflatoxin B1 and ochratoxin A, with the final concentration of 20 microgram / L, the 12-hour ochratoxin A degradation rate of the Lysobacter sp. CW239 is 53.1%, and the 48-hour degradation rate reaches 99.8%; the 12-hour aflatoxin B1 degradation rate of the Lysobacter sp. CW239 is 42.5%, and the 48-hour degradation rate reaches 83.4%; when the Lysobacter sp. CW239 is used for processing feed (with the final concentration of 20 microgram / kg) polluted by toxins, the 48-hour ochratoxin A degradation rate is 68.7%, and the 48-hour aflatoxin B1 degradation rate is 52.1%; the Lysobacter sp. CW239 has substantial application value and significance when being applied to food and feed bio-detoxification.

Description

technical field [0001] The invention relates to the fields of microbiology and biodegradation, in particular to a lysobacterium that efficiently degrades aflatoxin B1 and ochratoxin A and an application thereof. Background technique [0002] Aspergillus mycotoxins are mainly secondary metabolites produced by Aspergillus flavus, A. (OTA) is the most toxic and most widely polluted in agricultural production and food industry. In 1993, aflatoxin was classified as a Class I carcinogen by the World Health Organization (WHO) Cancer Research Institute. Because of its strong carcinogenic, teratogenic and mutagenic properties, aflatoxin has gradually become the focus of public health and scientific research. Contamination of grain and oil crops with aflatoxins and ochratoxins has become a global problem. In animal husbandry, the two types of aspergillus can endanger animal health by contaminating feed, leading to low productivity in animal husbandry, causing serious economic losses,...

AI Technical Summary

Problems solved by technology

Most of the virus-free strains reported so far have the following two common problems in practical application: First, the detoxification mechanism of some virus-free strains belongs to physical adsorption, not substantial biodegradation, and is adsorbed on the cell wall of the bacteria detoxification may occur under different physical and chemical environments in animals or humans, and has no substantial detoxification significance; second, the detoxification rate of the reported strains to toxins is mostly measured at a higher concentration of 100 μg / kg or more. There are few reports on the actual detoxification ability of low-concentration aspergillus toxoids (less than 20 μg / kg) in complex matrices such as food raw materials and feedstuffs

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