Pleurotus ostreatus and its application in aflatoxin degradation

A technology for aflatoxin and oyster mushroom is applied to oyster mushroom and its application field in degrading aflatoxin, and can solve the problems of affecting animal embryos, loss of export trade, affecting the export of peanuts and their products, etc.

Active Publication Date: 2013-07-24
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aflatoxin B 1 Also affects animal embryos, causing immunosuppression and recurrent infections, reducing milk and egg production in some animals
Aflatoxins mainly exist in crops such as corn and peanuts, which seriously affect the export of peanuts and their products in my country, and have brought huge losses to my country's export trade.

Method used

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  • Pleurotus ostreatus and its application in aflatoxin degradation
  • Pleurotus ostreatus and its application in aflatoxin degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The acquisition and identification of embodiment 1, flat mushroom LY65

[0027] 1. Obtaining Pleurotus ostreatus LY65

[0028] 1. In March 2011, in the ultra-clean workbench, different edible fungi were inoculated on GMY solid medium plates, and cultured in a constant temperature incubator at 30°C for 5-6 days. The strains covered the entire plate and were taken out. Store in a 4°C refrigerator for later use.

[0029]2. Use a hole puncher with a diameter of 7 mm to punch a hole in the center of the plate of the activated strain, take 4 pieces and inoculate them into a 250 mL Erlenmeyer flask containing 100 mL of liquid medium, and culture at 30°C and 200 rpm for 9-10 days. Then the fermentation broth was centrifuged at 10000rpm for 5min to obtain the supernatant, which was applied to degrade aflatoxin B 1 test, thus screening for aflatoxin B 1 The bacterial strain with the best degradation ability is named as LY65 (screened from Pleurotus ostreatus).

[0030] 2. Ide...

Embodiment 2

[0035] Embodiment 2, the preparation of flat mushroom LY65 fermented liquid

[0036] Cultivate Pleurotus LY65 on the GMY solid medium plate for 5 days, then punch holes with a diameter of 7mm to prepare a mycelial circle, get 4 pieces of mycelial circles and inoculate them into 100mL MSM liquid medium, at 30°C, 200rpm ( Shake culture for 9 days under the condition of rotation radius 26mm), take the fermentation system, and centrifuge at 10000rpm for 5min to obtain the fermentation broth.

Embodiment 3

[0037] Embodiment 3, flat mushroom LY65 fermented liquid is to the aflatoxin B in the solution 1 Degradation test of

[0038] Aflatoxin B 1 Standard product: sigma company article number A6636.

[0039] 1. Aflatoxin B 1 The standard substance was dissolved in acetonitrile (chromatographically pure) to obtain aflatoxin B with a concentration of 100 μg / mL 1 standard solution.

[0040] 2. Pipette 790 μL of the fermented broth prepared in Example 2 (using the MSM liquid medium as the contrast of the fermented broth) and 10 μL of the aflatoxin B1 standard solution with a concentration of 100 μg / mL (the total volume of the fermented broth and the standard solution is 800 μL, The total mass is 1g; aflatoxin B 1 The initial concentration in the total system is 1250ng / g), added to a 1.5mL sterilized centrifuge tube, reacted at 30°C and 200rpm for 72h, took samples, and used aflatoxin B 1 ELISA kit (Beijing Huaan Maike Biotechnology Co., Ltd. HE09004-1, the specific operation is ...

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PUM

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Abstract

The invention discloses a Pleurotus ostreatus and its application in aflatoxin degradation. The Pleurotus ostreatus LY65 provided by the invention is called LY65 for short, and has a preservation number of CGMCC No.5428. A broth of the LY65 can be used for degrading aflatoxin B1, has a high degradation capability, and is nontoxic and innocuous to humans and animals because LY65 strains are edible strains. LY65 and its broth have good application prospects in the aflatoxin B1 degradation.

Description

technical field [0001] The invention relates to a flat mushroom and its application, in particular to the flat mushroom and its application in degrading aflatoxin. Background technique [0002] Aflatoxin (AFT) is a secondary metabolite produced by fungi such as A.flavus, A.parasiticus and A.nomius. AFB has been isolated 1 、AFB 2 、AFG 1 、AFG 2 、AFM 1 、AFM 2 、AFB 2a 、AFG 2a 、AFBM 2a and AFGM 2a There are as many as 18 kinds, among which AFB 1 most toxic. A large number of epidemiological investigations and studies have confirmed that aflatoxin B 1 The main target organ is the liver, which can lead to liver necrosis and the formation of liver cancer. Aflatoxin B 1 It can also affect animal embryos, cause immunosuppression and repeated infections, and reduce milk and egg production in some animals. Aflatoxins mainly exist in crops such as corn and peanuts, which seriously affect the export of peanuts and their products in my country, and have brought huge losses to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04A62D3/02C12R1/645A62D101/28
Inventor 刘阳王会娟邢福国
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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