Trichoderma.citrinoviride strain XZ0509 and application thereof

A technology of Trichoderma citrus viridans and strains, applied in the direction of fungi, microorganisms, microorganisms, etc., can solve the problems of not having the ability to degrade aflatoxin and different aflatoxin capabilities

Active Publication Date: 2021-01-01
西藏自治区农牧科学院农业质量标准与检测研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many articles have successively reported that some species of the bacteria (T. pseudoconii, Trichoderma konii, Trichoderma reesei, Trichoderma viride, Trichoderma harzii) have varying degrees of aflatoxin Degradation,

Method used

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  • Trichoderma.citrinoviride strain XZ0509 and application thereof
  • Trichoderma.citrinoviride strain XZ0509 and application thereof
  • Trichoderma.citrinoviride strain XZ0509 and application thereof

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Embodiment 1

[0022]The strain is a strain isolated from the soil of Chayu County, Linzhi City, Tibet by the "Institute of Agricultural Quality Standards and Testing, Tibet Autonomous Region Academy of Agriculture and Animal Husbandry Sciences", and is produced by Shenzhen Huada Gene Co., Ltd. (https: / / en.genomics.cn / ), according to the internal transcribed spacer ITS (Internal transcribed spacer) sequence for bacterial species identification. The detailed information of each strain is shown in Table 1.

[0023]For strain morphological identification, refer to "Fungi Identification Manual". For molecular biology identification, total DNA was extracted using TIANGEN Fungal Genome Extraction Kit, and then used for the amplification of the target gene ITS. Fungal PCR amplification uses universal primers ITS1 / ITS4. The conventional amplification system is 25μl, and the amplification procedure is as follows: 95℃ 5min; 95℃ 30s, 58℃ 30s, 72℃ 1min, 35 cycles; 72℃ 7min. Take 2μL of PCR product and perform p...

Embodiment 2

[0028]2.1 Screening of Trichoderma strains that efficiently degrade aflatoxin

[0029]Trichoderma activation: autoclave the potato dextrose agar (PDA) medium at 121°C for 15 minutes, pour it into a Petri dish with a diameter of 9.0 cm, cool and solidify for later use. All Trichoderma is stored in 5% glycerol / water in advance, pick the Trichoderma hyphae from it or transfer 10μL of the preservation solution containing Trichoderma spores to the PDA medium, under 28±1℃, 90% humidity, 24h light conditions (Note: It can also be cultured in dark conditions for 5 days;

[0030]After autoclaving the YPD liquid medium at 121°C for 15 minutes, add AFB1 standard to the YPD liquid medium to a final concentration of AFB1 of 50ppb. Add the YPD medium containing AFB1 to a 12-well cell culture plate, 2.0 mL per well; then, Use a hole puncher to punch out round clumps with a diameter of 6.0 mm from the edge of the 5-day-old Trichoderma colony, and inoculate them into the cell culture plate holes, with 1 c...

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Abstract

The invention provides a trichoderma.citrinoviride strain XZ0509 and application thereof, and relates to the technical field of aflatoxin degradation. The preservation number of the strain XZ0509 is CCTCC M 2020522. The invention further provides the application of the strain XZ0509 in degradation of aflatoxin B1. After the strain XZ0509 is cultured for 3 days, the rate of degradation on an AFB1 solution with the concentration of 50ppt is greater than 80%, after the strain XZ0509 is cultured for 7 days, the AFB1 removal rate is 100%, and after the strain XZ0509 is cultured for 5 days, the rateof degradation on 10ppm AFB1 is still higher than 50%. The strain has strong bacteriostatic and toxin-producing-inhibiting effects at the same time, the inhibition rate on aspergillus flavus hypha is85.6%, and the rate of inhibition on toxin production of aspergillus flavus in peanuts is 92.8%. It is shown that the strain XZ0509 can be applied to degradation of aflatoxin in seriously polluted grain and oil products.

Description

Technical field[0001]The invention belongs to the technical field of aflatoxin degradation, and specifically relates to a Trichoderma citrinum strain XZ0509 and an application thereof.Background technique[0002]Aflatoxin is a secondary metabolite mainly produced by Aspergillus flavus and Aspergillus parasitica. The probability of aflatoxin in food and feed is highest in hot and humid regions. They exist in soil, animals and plants, and various nuts, especially grain and oil products such as peanuts, corn, rice, soybeans, and wheat, etc. They are the most toxic mycotoxins and are extremely harmful to human health. Aflatoxin B1 is the most common in natural foods, and it is also the most harmful.[0003]At present, in the control of Aspergillus flavus, chemical control occupies an important position. However, its cost is high, it is easy to pollute the environment, and while preventing and controlling pathogenic bacteria, the pathogenic bacteria are also easy to develop drug resistance o...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L5/20C12R1/885
CPCC12N1/14A23L5/28C12R2001/885C12N1/145
Inventor 魏娜张奇岳晓凤任显凤王军张飞龙达娃卓玛
Owner 西藏自治区农牧科学院农业质量标准与检测研究所
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