Method of degrading aflatoxin in peanut meal and synchronously preparing peanut protein peptide

A technology of aflatoxin and peanut protein, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of unreported multi-bacteria combined on aflatoxin degradation, strains are not easy to grow and reproduce, and affect consumers. Health and other issues, to achieve the effect of shortening fermentation time, easy industrial production, and shortening process time

Active Publication Date: 2018-05-15
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few studies on the degradation of aflatoxins in complex matrices such as peanut meal.
[0005] The strains involved in biodegradation currently mainly involve domestic Pseudoarmillaria, Myxobacteria, Stenotrophomonas maltophilia, and foreign researched Flavobacterium aurantium and Rhodococcus, etc. Growth and reproduction, low degradation rate, some strains cannot be used in food production, and there are certain safety hazards, etc.
And it is all about the degradation of aflatoxin by a single strain of bacteria, and the degradation of aflatoxin by a combination of multiple bacteria has not been reported
[0006] At the same time, peanut meal contains a large amount of protein and fiber, and there are many reports on the preparation of protein peptides in peanut meal, but there is no report on the removal of aflatoxin in protein, resulting in the presence of aflatoxin in the prepared protein peptides, affecting consumer health

Method used

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  • Method of degrading aflatoxin in peanut meal and synchronously preparing peanut protein peptide
  • Method of degrading aflatoxin in peanut meal and synchronously preparing peanut protein peptide
  • Method of degrading aflatoxin in peanut meal and synchronously preparing peanut protein peptide

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Experimental program
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Effect test

Embodiment 1

[0052] The total amount of aflatoxins in peanut meal raw materials is 92 μg / kg (wherein B 1 is 55 μg / kg, B 2 is 16 μg / kg, G 1 is 19 μg / kg, G 2 is 2 μg / kg), and the cyclopiazonic acid content is 52 μg / kg. Add 10g of peanut meal to 20mL of water, sterilize, inoculate 1g of Versicolor versicolor strain activated by peanut root fermentation, solid fermentation at 25°C for 8 days, then inoculate 1mL of Bacillus subtilis strain activated by peanut meal aqueous solution into the fermented peanut meal, Solid fermentation at 30°C for 3 days, then inoculate peanut meal with 1g of Aspergillus niger activated from peanut roots, solid fermentation at 35°C for 3 days; add 30mL of distilled water to the fermented medium, shake at 33°C for 2 hours, then shake at 25°C for enzymolysis 8h; the enzymolysis solution was treated at 100°C for 15 minutes, cooled to room temperature and centrifuged at 10,000rpm for 10 minutes; Air volume is 22m 3 / h, after the drying is completed, the peanut prot...

Embodiment 2

[0057] The total amount of aflatoxins in peanut meal raw materials is 92 μg / kg (wherein B 1 is 55 μg / kg, B 2 is 16 μg / kg, G 1 is 19 μg / kg, G 2 is 2 μg / kg), and the cyclopiazonic acid content is 52 μg / kg. Add 10g of peanut meal to 20mL of water, sterilize, inoculate 0.7g of Versicolor versicolor strain activated by peanut root fermentation, solid fermentation at 28°C for 6 days, and inoculate 0.7 Bacillus subtilis strain activated by peanut meal aqueous solution into the fermented peanut meal mL, solid fermentation at 32°C for 4 days, then inoculate peanut meal with 0.7 g of Aspergillus niger activated from peanut roots, and solid fermentation at 32°C for 4 days; add 40 mL of distilled water to the fermented medium, shake and enzymolyze at 34°C for 1.5 hours, 23 ℃ shaking enzymolysis for 10 hours; enzymatic hydrolysis solution at 100 ℃ for 15 minutes, cooling to room temperature and centrifuging at 10,000 rpm for 10 minutes in a high-speed centrifuge; spray drying the supern...

Embodiment 3

[0062] The total amount of aflatoxins in peanut meal raw materials is 92 μg / kg (wherein B 1 is 55 μg / kg, B 2 is 16 μg / kg, G 1 is 19 μg / kg, G 2 is 2 μg / kg), and the cyclopiazonic acid content is 52 μg / kg. Add 10g of peanut meal to 25mL of water, sterilize, inoculate 0.7g of Versicolor versicolor strain activated by peanut root fermentation, solid fermentation at 28°C for 6 days, and then inoculate 0.7 Bacillus subtilis strain activated by peanut meal aqueous solution into the fermented peanut meal mL, solid fermentation at 32°C for 4 days, then inoculate peanut meal with 0.7 g of Aspergillus niger activated from peanut roots, and solid fermentation at 32°C for 4 days; add 40 mL of distilled water to the fermented medium, shake at 35°C for 1 hour, then inoculate at 22°C Shake enzymatic hydrolysis for 12 hours; enzymatic hydrolysis solution was deactivated at 100°C for 15 minutes, cooled to room temperature, and centrifuged at 10,000 rpm for 10 minutes in a high-speed centrifu...

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Abstract

The invention discloses a method of degrading aflatoxin in peanut meal and synchronously preparing peanut protein peptide and belongs to the field of food safety and nutrition of peanuts. The method includes the steps of: raw material treatment, sectional microbial fermentation, enzymolysis, enzyme deactivation and centrifugation, and spray drying, wherein microorganisms employed in the sectionalmicrobial fermentation comprise coriolus versicolor, bacillus subtilis and aspergillus niger. In the method, the three microorganisms achieve synergistic sectional fermentation and sectional enzymolysis, so that during degradation of the aflatoxin, cyclopiazonic acid is also degraded, and the peanut protein peptide having high anti-oxidizing activity and high nutritional value is prepared. The method is reduced in operation process and shortened in process time, saves cost, has simple operations and is easy to carry out industrially.

Description

technical field [0001] The invention belongs to the field of peanut food safety and nutrition, and in particular relates to a method for degrading aflatoxin in peanut meal and synchronously preparing peanut protein peptides. Background technique [0002] my country is a large peanut producing country, and about 3 million tons of peanut meal can be obtained after peanut oil extraction every year. Peanut meal is rich in nutrients, such as peanut protein, flavonoids, sugars and so on. Among them, compared with soybean protein, which is widely used, peanut protein has the advantage of containing less flatulence factors and anti-nutritional factors; compared with rapeseed and cottonseed protein, it has the advantage of containing less toxic substances. However, the utilization rate of peanut meal is low, and it is mainly used as feed, and the price is low. Now it is about 2,600 yuan / ton, which is far lower than the price of soybean meal (3,200 yuan / ton). The main factor limitin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P21/00C12P21/06A23L5/20C12R1/685C12R1/645C12R1/125
CPCA23L5/25A23L5/28A23L29/065A23L33/00A23V2002/00C12P21/00C12P21/06C12P39/00A23V2200/30A23V2200/324A23V2200/302A23V2200/322A23V2200/326A23V2200/3262
Inventor 张初署张建成杨庆利孙杰王明清于丽娜毕洁
Owner SHANDONG PEANUT RES INST
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