Preparation method of tobacco extractum and use thereof
A technology of tobacco extract and tobacco powder, which is applied in the field of preparation of tobacco extract, can solve the problems of high cost and high difficulty in industrial production, and achieve the effects of low production cost, low price and high purity
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Embodiment 1
[0027] Weigh 30g of tobacco powder into a 500mL triangular flask, add distilled water at a solid-to-liquid ratio of 1:5, wrap the mouth of the bottle with gauze, and sterilize and cook for 30 minutes in steam at 100°C in a sterilizing pot.
[0028] Adjust the pH value to 6.0, respectively add cellulase with a weight of 2‰ of tobacco dust and liquefaction enzyme with a weight of 5‰ of tobacco dust, and react in a constant temperature water bath shaker at 50°C and 150r / min for 4h.
[0029] Adjust the pH value to 4.5, add 1% glucoamylase by the weight of tobacco powder, and react at 50° C., 150 r / min in a constant temperature water bath shaker for 3 hours.
[0030] Inject the corresponding strain seed solution into the enzymatic hydrolyzate after the enzyme treatment in proportion, and cultivate it in a constant temperature shaker at 30°C and 120r / min for 3 days. (Lactobacillus delbrueckii inoculation amount 1%; Rhizopus inoculum amount 3%, Aroma yeast inoculum amount 1%)
[003...
Embodiment 2
[0036] Weigh 30g of tobacco powder into a 500mL triangular flask, add distilled water at a solid-to-liquid ratio of 1:7, wrap the mouth of the bottle with gauze, and sterilize and cook in steam at 100°C for 40 minutes in a sterilizing pot.
[0037] Adjust the pH value to 7.0, respectively add cellulase with a weight of 5‰ of tobacco dust and liquefaction enzyme with a weight of 3‰ of tobacco dust, and react in a constant temperature water bath shaker at 60°C and 150r / min for 2h.
[0038] Adjust the pH value to 8.0, add 2% glucoamylase by weight of tobacco powder, and react at 70°C and 150r / min constant temperature water bath shaker for 1.5h.
[0039] Inject the corresponding strain seed solution into the enzymatic hydrolyzate after the enzyme treatment in proportion, and cultivate it in a constant temperature shaker at 50°C and 120r / min for 3 days. (Lactobacillus delbrueckii inoculum 2%; Rhizopus inoculum 1%, Aroma yeast inoculum 2%)
[0040] Take out the fermentation broth a...
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