Method for producing trichoderma chlamydosporium

A technique for chlamydospore and production method, which is applied in the directions of botanical equipment and methods, fungi, biocides, etc., can solve the problems of short commodity price period, short spore survival period, unstable control effect, etc., and achieve the survival time. Long-term, simple and easy to implement, competitive in the soil and the effect of high reproductive speed

Inactive Publication Date: 2005-03-16
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of production method of the Trichoderma chlamydospore that is used for preventing and treating plant disease, to overcome the spore survival period of the conidia preparation, the commodity price period is short, and the control effect brought by it Instability and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Preparation of slant mother strains

[0022] Take 5 grams of crop root surface soil, add 50 ml of sterile water, dilute 1000 times after shaking to form a dilution, take 0.1 ml of the dilution and 10 ml of the mother strain culture solution and pour it into a petri dish to make a culture medium plate. Among them, the culture solution of the mother strain is taken: 150 grams of potatoes, 20 grams of glucose, 0.05 grams of Bengal red, 0.03 grams of streptomycin sulfate, 17 grams of agar, and 1000 ml of water. The production method is: peel the potatoes and cut them into 1 cm squares Add 1000 ml of water and boil for 20 minutes, filter the potato pieces with gauze, add agar, add water to make up 1000 ml, then add glucose and Bengal red and make it completely melted, then put it into a triangular flask for sterilization, at 121 °C Sterilize for 30 minutes and add streptomycin sulfate before inverting the plate. Cultivate at 25°C for 48 hours, obtain the Trichoderma stra...

Embodiment 2

[0029] 1. Preparation of slant mother strains

[0030] Take 10 grams of crop root surface soil, add 100 ml of sterile water, dilute 10,000 times after shaking to form a dilution, take 1 ml of the dilution and 15 ml of the mother strain culture solution and pour it into a petri dish to make a culture medium plate. Among them, the culture solution of the mother strain is taken: 200 grams of potatoes, 20 grams of glucose, 0.06 grams of Bengal red, 0.04 grams of streptomycin sulfate, 20 grams of agar, and 1000 milliliters of water. The production method is: peel the potatoes and cut them into 1 cm squares Add 1000 ml of water and boil for 30 minutes, filter the potato pieces with gauze, add agar, add water to make up 1000 ml, then add glucose and Bengal red and make it completely melted, then put it into a triangular flask for sterilization, at 121 °C Sterilize for 30 minutes and add streptomycin sulfate before pouring the plate. Cultivate at 28°C for 48 hours, obtain the Trichod...

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Abstract

A trichoderma spp chlamydospore production method of obtaining trichoderma spp from crop root surface soil is disclosed. The method obtains chlamydospore through controlling nutrition condition , adding chlamydospore promotor, regulating other technical parameter to control the fermentation condition. First the trichoderma spp from corp root is purified and then transferred to tube slant as stock bacteria, then vibrated in triangular tube to become bacteria liquid, at last fermented in fermenter to produce chlamydospore suspension liquid. The merit of inventive chlamydospore is long life.

Description

technical field [0001] The invention relates to a production method of a biocontrol fungus-Trichoderma harzianum (Trichoderma harzianum) chlamydospore used for preventing and treating plant diseases, specifically a method for making Trichoderma harzianum produce chlamydospore by controlling nutrient conditions and fermentation conditions. Background technique [0002] As more and more toxic chemical pesticides are gradually restricted in use, countries all over the world are increasing the research and development of biological pesticides, and vigorously advocating the research and development of microbial pesticides that are non-toxic to humans and animals and non-polluting to the environment has become a hot spot . As a class of antagonistic fungi with important biocontrol value, Trichoderma has been found to have antagonistic effects on plant pathogenic fungi as early as the 1930s, and its mechanism of action includes antibiotic production, parasitism, bacteriolysis, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04C12N1/14
Inventor 陈捷庄敬华高增贵
Owner SHANGHAI JIAO TONG UNIV
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