A morchella strain liujie k

Abstract

The application discloses a Morchella sextelata strain Liujie K. The strain is preserved in the China Typical Culture Collection Center of Wuhan University in Wuhan, China on October 25, 2022, and the preservation number is CCTCC NO: M20221647. The application screens a new Morchella sextelata strain Liujie K which has strong stress resistance, good stability, low hybridization rate of 1% and high yield. The strain has strong adaptability, can guarantee yield under different climate conditions and different cultivation modes, and can be used as a new excellent strain for Morchella sextelata cultivation in most areas of China.

Description

Technical Field

[0001] This invention belongs to the field of edible fungi technology, specifically relating to a morel (Morchella sextelata) strain, strain 6mei K, which has strong stress resistance and good stability. Background Technology

[0002] Morel mushrooms belong to the phylum Ascomycota, class Discomycetes, order Pezizales, family Morchellaceae, and genus Morchella in the classification of edible fungi. Commonly known as sheep mushrooms or sheep stomach vegetables, they are named for their honeycomb-like surface and stomach-like shape. Rich in nutrients and high in nutritional value, they are a popular and high-end food ingredient, highly favored by consumers.

[0003] Currently, commercial large-scale field cultivation of morel mushrooms in my country has been ongoing for over a decade, and the varieties have gradually become more diverse. From the earliest large-scale cultivated morel variety, the Tiling variety, to the Liumei variety, and now the Qimei variety, more and more new varieties are emerging. However, morel mushrooms belong to the ascomycetes, and their genetic characteristics are inherently prone to degeneration, aging, and mutation. This means that the longer a morel variety is used, the more likely it is to experience degeneration, aging, and mutation, leading to various problems in production. Therefore, it is necessary to continuously select and breed superior new varieties with good resistance, stability, high yield, and good quality to meet market demands. The most direct manifestation of large-scale yield reductions or crop failures in artificial morel cultivation in recent years is the infection rate of the nutrient bags (i.e., the nutrient bag contamination rate). A higher nutrient bag contamination rate during the mycelium growth period poses a greater risk to yield later on. Therefore, selecting a highly adaptable strain can effectively control the nutrient bag contamination rate and reduce cultivation risks.

[0004] Based on years of research and practical experience, some risks in morel cultivation lie in the spawn, which originates from the strain or variety. The most important aspect of a good strain is its strong adaptability. Strong adaptability means being able to produce fruiting mushrooms well under different climatic conditions, cultivation methods, and adverse factors. Two crucial aspects of strong adaptability are high resistance to adverse conditions and good stability. Currently, many morel varieties cultivated domestically do not demonstrate particularly outstanding performance in these areas. Therefore, there is an urgent need to obtain a morel variety with excellent resistance to adverse conditions and good stability. Summary of the Invention

[0005] In view of the above-mentioned shortcomings in the prior art, the present invention provides a morel (Morchellasextelata) strain with strong stress resistance, high stability, high yield and good quality, which can be used as a new superior strain for morel cultivation in most parts of my country.

[0006] To achieve the above objectives, the technical solution adopted by the present invention to solve its technical problem is as follows:

[0007] A highly resistant morel (Morchella sextelata) strain, known as "Six Sister K", was deposited on October 25, 2022, at the China Center for Type Culture Collection, Wuhan University, Wuhan, China, with accession number CCTCCNO:M 20221647.

[0008] The fruiting bodies, mycelia, or spores produced by the aforementioned morel mushroom K.

[0009] Furthermore, the child entities can be solitary or clustered.

[0010] Furthermore, the fruiting body is stomach-shaped, with a white, hollow stalk and the rest being black.

[0011] Furthermore, the cap of the fruiting body is oblong-conical in shape.

[0012] The above-mentioned method for cultivating morel mushroom 'Six Sister K' involves inoculating the strain into PDA medium and culturing it at 20–25°C.

[0013] Furthermore, the PDA medium comprises potato at a final concentration of 100–200 g / L, glucose at a final concentration of 10–50 g / L, and agar at a final concentration of 20–50 g / L.

[0014] Uses of the fruiting bodies and / or mycelia produced by the above-mentioned morel mushroom K in food processing

[0015] The beneficial effects of this invention are:

[0016] This invention screened and obtained a new morel strain, Liu Mei K, which has strong stress resistance, good stability, low contamination rate of 1%, and high yield. It is highly adaptable and can guarantee yield under different climatic conditions and different cultivation methods. Attached Figure Description

[0017] Figure 1 It is the offspring of Six Sister K in its wild state;

[0018] Figure 2 The fruiting body characteristics of morel strain 6-mei K are shown; among them, 2-1 is a cross-sectional view of the fruiting body of 6-mei K; 2-2 and 2-3 are characteristic images of the fruiting body of 6-mei K.

[0019] Figure 3 Characteristics of the hyphae and ascospores of morel strain 6-mei K;

[0020] Figure 4 Colony and hyphal characteristics of morel strain 6-mei K;

[0021] Figure 5The characteristics of the sclerotium of Morel strain 6-Sister K;

[0022] Figure 6 Comparison of mycelial characteristics of various morel strains in culture dishes after 7 and 10 days; in each image, the left image is the result of 7-day culture and the right image is the result of 10-day culture;

[0023] Figure 7 Images show the mycelial characteristics of some strains at 15 and 20 days in cultivation; in each image, the left image shows the mycelial characteristics at 15 days, and the right image shows the mycelial characteristics at 20 days.

[0024] Figure 8 The image shows a comparison of bacterial frost formation on strain 6-mei K and the control strain after 30 days; from left to right in the image are 6-mei K, 6-mei control 1, and 7-mei control 2.

[0025] Figure 9 Comparison of the contact surfaces of strain 6-mei K and control strain 6-mei K with soil after 30 days in nutrient bags; from left to right in the figure are strain 6-mei K, strain 6-mei control 1 and strain 7-mei control 2.

[0026] Figure 10 This is a developmental diagram of the fruiting body of the fruiting body of *Six Sister K*. From left to right, the images show the primordial stage, juvenile stage, growth stage, and maturity stage of the fruiting body of *Six Sister K*.

[0027] Figure 11 The image shows the development of the fruiting body of the sixth sister (K) fruiting body (comparison 1). From left to right, the images depict the primordial stage, young fruiting body stage, growth stage, and maturity stage of the sixth sister (K) fruiting body.

[0028] Figure 12 The image shows the development of the fruiting body of the seventh sister (Seventh Sister) compared to the second sister (Seventh Sister). From left to right, the images show the primordial stage, juvenile stage, growth stage, and maturity stage of the fruiting body of the sixth sister (Sixth Sister) (K).

[0029] Figure 13 This is the ISSR fingerprint of the primers for strain Y12; the upper and lower lines represent the positions of the 5000bp and 20bp nucleic acid bands, respectively; number 5 is the selected sixth sister K, number 1 is the sixth sister control 1, and number 8 is the seventh sister control 2;

[0030] Figure 14 The ISSR genetic clustering analysis diagram of 35 strains is shown; among them, No. 5 is the selected sixth sister K, No. 1 is the sixth sister control 1, and No. 8 is the seventh sister control 2;

[0031] Figure 15 Comparison of fresh mushrooms between Liu Mei K and Liu Mei Control 1; the left image is Liu Mei K, and the right image is Liu Mei Control 1.

[0032] Figure 16 Comparison images of dried mushrooms: Six Sister K and Six Sister Control 1; the left image is Six Sister K, and the right image is Six Sister Control 1. Detailed Implementation

[0033] The specific embodiments of the present invention are described below to enable those skilled in the art to understand the present invention. However, it should be understood that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, various changes are obvious as long as they are within the spirit and scope of the present invention as defined and determined by the appended claims. All inventions utilizing the concept of the present invention are protected.

[0034] 1. Terminology Explanation:

[0035] Fruiting body: The sporulation structure of higher fungi, i.e., the fruiting body, is composed of organized mycelium.

[0036] Primordial: Before edible fungi develop fruiting bodies, small granules are formed by the entanglement and twisting of mycelium. These granules eventually grow into individual mushrooms.

[0037] Hyphae: Single tubular filaments, the structural unit of most fungi.

[0038] Mycelium: Many mycelia aggregate together to form the vegetative body of fungi, namely mycelium.

[0039] 2. The culture medium formulation used in the embodiments of the present invention is as follows:

[0040] PDA medium: 200g potato, 20g glucose, 20g agar, 1L water.

[0041] Original culture medium: 40% sawdust, 35% wheat grains, 23% soil, 1% lime, and 1% gypsum (by weight).

[0042] Culture medium for cultivation: 40% sawdust, 35% wheat grains, 23% soil, 1% lime, and 1% gypsum (by weight).

[0043] The culture medium formula in the nutrient bag is: 30% wheat grains, 68% rice husks, 1% lime, and 1% gypsum (by weight).

[0044] Unless otherwise specified, the experimental conditions in the examples are generally as per conventional conditions or the conditions recommended by the reagent company. Unless otherwise specified, the reagents and consumables used in the following examples are all commercially available.

[0045] Example 1: Collection, isolation, and identification of bacterial strains

[0046] 1. Strain collection and isolation

[0047] In May 2019, we collected wild morel resources in Shangri-La, Yunnan Province, and Kangding County, Ganzi Prefecture. This included surveying the ecological environment of wild morels and collecting their fruiting bodies. Eight wild morel strains were isolated and purified from the collected fruiting bodies. We also collected 27 morel strains from field-cultivated varieties across China, including the Liumei, Tilen, and Qimei series. We conducted comparative experiments on a total of 35 wild and cultivated strains.

[0048] Wild morel mushrooms were collected and isolated in Kangding County, Ganzi Prefecture, and a pure mycelial strain with the identification number "K" was obtained (see Table 1 below). The wild morel mushroom K has a solitary, black, hollow fruiting body with a white stipe, reaching a height of 12 cm. It was collected from a mountainside, growing in shrubland and mixed forest, in a relatively thick and moist humus-rich environment. The vegetation in its habitat mainly consisted of wild poplar, birch, and oak trees (see Table 1 below). Figure 1 ).

[0049] Table 1 shows the sources and numbers of the collected strains.

[0050]

[0051]

[0052] Among them, serial number 5 is strain "Liu Mei K", serial number 1 is control strain 1 Liu Mei, and serial number 8 is control strain 2 Qi Mei.

[0053] 2. Identification of the Six Sisters K strain

[0054] 2.1 Biological characteristics

[0055] (1) Sub-entity characteristics

[0056] The fruiting bodies are solitary or clustered, medium in size, 8–20 cm long. The cap is oblong-conical, 8–15 cm long and 4–6 cm wide. The surface is pitted, resembling a sheep's stomach, and is black. The stipe is white, hollow, 2–5 cm long and 1–2.5 cm wide, with shallow, fine, and relatively dense longitudinal grooves. The base is slightly swollen. The longitudinal section of the stipe is trapezoidal. (See...) Figure 2-1 , Figure 2-2 , Figure 2-3 )

[0057] (2) Characteristics of Ascospores

[0058] Ascospores: 250-300 μm × 17-20 μm. Ascospores: 8 in number, arranged in a single row, elliptical, 20-24 μm × 12-15 μm. (See appendix) Figure 3 )

[0059] (3) Colony and hyphae characteristics

[0060] Colonies on PDA medium are initially white, later darkening in color with denser hyphae. Growth is rapid, with the mycelium expanding relatively evenly outwards. The mycelium is white, filamentous, 4.0–6.0 μm in diameter, and septate. (See appendix) Figure 4 )

[0061] (4) Characteristics of sclerotia

[0062] It is initially white, turning yellow as it grows to a certain stage. (See appendix) Figure 5 )

[0063] (5) Temperature

[0064] The mycelium grows in a temperature range of 5–30℃, with an optimal growth temperature of 15–20℃; the fruiting body grows in a temperature range of 5–20℃, with an optimal growth temperature of 10–18℃.

[0065] (6) Light

[0066] Mycelial growth does not require light. Fruiting body formation and development require a certain amount of diffused light.

[0067] (7) Humidity

[0068] The water content of the culture medium for mycelial growth ranges from 50% to 80%, with the optimum water content being 65%. During the fruiting body formation and development stages, the suitable relative humidity is 85% to 90% and the soil moisture is 70% to 80%.

[0069] (8) Air

[0070] Mycelial growth requires relatively little oxygen; fruiting body growth and development require good ventilation.

[0071] (9) pH

[0072] The optimal pH range for mycelial and fruiting body growth is 6–8.

[0073] 2.2 DNA Molecular Identification

[0074] The strain K of the novel coronavirus was entrusted to Sangon Biotech (Shanghai) Co., Ltd. for strain identification. The DNA sequencing results of the novel coronavirus K are as follows (599bp):

[0075]

[0076]

[0077] After comparison with NCBI using blast, the strain was identified as Morchella (a genus of morel mushrooms), and presumed to be Morchellasextelata (a type of morel mushroom).

[0078] 2.3 ISSR fingerprinting and genetic differences between Six Sister K and the collected strains

[0079] Taq DNA polymerase and dNTPs were purchased from MBI Fermentas, and ISSR primers were synthesized by Sangon Biotech (Shanghai) Co., Ltd. The strain was activated and cultured at 20℃ for 7 days. Inoculum blocks were picked and inoculated onto liquid culture medium and cultured at 20℃ for 10 days. After washing the mycelia twice with sterile water, the surface moisture of the mycelia was blotted with sterile filter paper, and the mycelia were stored at -20℃ for later use. Mycelial DNA was extracted using the CTAB method. Amplification was performed on a BioRad C1000 PCR instrument. The reaction system was: 1U Taq enzyme, 2ul of 10×Buffer (200mM Tris-HCl; 100mM (NH4)2SO4; 200mM KCl; 15mM MgCl2), 40 ng template DNA, 0.2 mmol / L dNTPs, 0.2 μmol / L primers (primer sequences are shown in the table), and water was added to a final volume of 20ul. The amplification annealing temperature was 45-65℃. After electrophoretic separation and imaging, band statistics were performed. ISSR bands in the ISSR spectrum were counted as 1, and those without ISSR bands as 0, to construct an initial data matrix. The Nei & Li coefficients were then calculated using NTSYSpc2.1 professional software.

[0080] Table 2 ISSR Primers

[0081]

[0082]

[0083] The experiment yielded 12 primers that amplified clear, polymorphic, and highly reproducible DNA bands, resulting in a total of 114 clearly identifiable polymorphic DNA bands. Each primer amplified between 1 and 11 DNA bands. The size of the amplified DNA bands ranged from 100 to 1500 bp (see appendix). Figure 13 ).

[0084] The ISSR fingerprints of the strains were converted into a 0,1 data matrix, and the Nei & Li coefficient (1979) was calculated using NTSYSpc2.1 professional software to calculate the genetic dissimilarity coefficient. The results showed that the genetic similarity coefficient among the 35 strains was 0.091, meaning there was a genetic distance of 0.909 among the 35 strains, indicating a relatively large overall genetic difference (see Appendix). Figure 14 ).

[0085] According to the analysis of the genetic clustering results, 35 strains are clustered into 7 groups. Among them, the strains numbered 1, 2, 3, 4, 5, 6, 7, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 31, 33, and 34 are clustered into one group, which are all the Liu-mei line strains collected by us (green area). The Liu-mei K bred by us is numbered 5 and also belongs to the Liu-mei line. Strains numbered 22, 23, and 24 are clustered into one group, which are wild strains collected by us from Pakistan; strains numbered 26 and 27 are clustered into one group, which are wild strains collected by us from Sichuan; strains numbered 25 and 28 are clustered into one group, which are wild strains collected by us from Yunnan; strains numbered 8, 9, 16, 20, 32, and 35 are clustered into one group, which are the Qi-mei line strains collected by us (blue area); strains numbered 21 and 30 are clustered into one group, which are the Ti-leng line strains collected by us (yellow area); Strain No. 29 has a relatively far genetic distance from other strains and forms a separate cluster, which is a Qi-mei line strain collected by us.

[0086] We analyzed the genetic relationship of 35 Morchella strains by ISSR method. The analysis based on the genetic clustering diagram results shows that the overall genetic difference between the Liu-mei K strain and other Liu-mei line strains is relatively large, and the overall genetic difference between Liu-mei K and strains of other lines is even larger. This method can clearly and detailedly distinguish these strains and quantify the genetic differences between Liu-mei K and other strains. Liu-mei K shows significant differences from Liu-mei control strain 1 and Qi-mei control strain 2 in the genetic clustering analysis diagram, and it belongs to a different strain.

[0087] Finally, on October 25, 2022, the screened Morchella strain Liu-mei K was deposited at the China Center for Type Culture Collection, Wuhan University, Wuhan, China, with the deposit number CCTCC NO: M 20221647.

[0088] Example 2: Observation and comparison of mycelial and sclerotial characteristics of 35 strains in culture dishes

[0089] Each collected strain was used for mycelial culture in a 9 cm culture dish, the culture medium formula is PDA, and the culture is carried out at a culture temperature of 20°C. 5 repeated plates are set for the culture dish corresponding to each strain. The mycelial germination and growth of each strain were observed and recorded, including the following contents: the development degree of aerial hyphae after 7 days of growth; bacteria after 7 days of growth; sclerotia color; distribution pattern of sclerotia; pigment formation after 10 days of growth; growth length of mycelia after 3 days of growth. The mycelial growth data of each strain are compared (see Table 3).

[0090] Table 3 Mycelial growth data of various Morchella strains

[0091]

[0092]

[0093] Among them, serial number 5 is strain "Liu Mei K", serial number 1 is control strain 1 Liu Mei, and serial number 8 is control strain 2 Qi Mei.

[0094] As shown in Table 3, the hyphae of *Symplocos chinensis* K on PDA medium were initially light gray, with relatively neat tips; the aerial hyphae were moderately to strongly developed, indicating vigorous growth; after 7 days of growth, no obvious sclerotia were observed in the culture dish; after 10 days of growth, the pigment produced by the hyphae was weak; and after 3 days of growth, the length of the mycelium in the culture dish was 2.4 cm, indicating a faster growth rate than the control *Symplocos chinensis*. (See appendix) Figure 6 )

[0095] Example 3: Comparison of mycelial and sclerotium characteristics of 35 strains on culture substrate

[0096] The collected strains were cultured in 12*24 cm inoculum bags for observation and comparison of mycelial and sclerotial characteristics. The inoculum formula was: 40% sawdust, 35% wheat grains, 23% soil, 1% lime, and 1% gypsum. The culture temperature was 20 degrees Celsius. Twenty replicates were made for each strain to observe and record the mycelial and sclerotial characteristics. This included: measuring the mycelial length after 3 days of growth for each strain; observing and recording the number of days from inoculation to the onset of sclerotial formation for each strain; observing and recording the sclerotial color after 15 days of growth for each strain; and recording the location of sclerotial formation in the inoculum bag. Detailed comparisons of mycelial growth data for each strain are shown in Table 4.

[0097] Table 4. Record of mycelial growth data for various morel mushroom strains and cultivation varieties.

[0098]

[0099]

[0100] Among them, serial number 5 is strain "Liu Mei K", serial number 1 is control strain 1 Liu Mei, and serial number 8 is control strain 2 Qi Mei.

[0101] As shown in Table 4, the growth length of "Liu Mei K" in the cultivation substrate after 3 days was 5.35 cm, which was significantly faster than the control "Liu Mei". The sclerotia began to form after 10 days, and the sclerotia were yellowish-white in color, mostly concentrated in the middle of the substrate bag. The growth characteristics of the "Liu Mei K" cultivar were similar to other "Liu Mei" strains. (See Appendix) Figure 7 )

[0102] Example 4: Field cultivation of main varieties and comparison of mushroom yield

[0103] The experimental plots were selected from fertile sandy soil. Shade netting arched sheds were constructed, and raised beds were made inside. The beds were 0.8m to 1m wide, with 30cm wide and 20cm deep furrows between them as walkways. The spawn was sown on the beds at a rate of approximately 800g (one bag of 18 spawn bags) per square meter, then covered with about 3cm of soil. The spawn was then evenly spread on the bed surface and covered with 3cm of soil. After sowing, water was sprayed to keep the bed surface moist at a depth of 20cm. Ten days later, nutrient bags (12*24 cm) were placed on the beds, four bags per square meter, and removed before fruiting. Three replicates were designed for each variety, with each replicate covering 100 square meters. The variety comparison test was conducted in Dayi County, Chengdu Plain. The commonly used field-cultivated strains Liu Mei and Qi Mei were selected as control strains for Liu Mei K. The control strains are numbered 1 (Sixth Sister Control 1) and 8 (Seventh Sister Control 2) in Tables 1, 2, and 3, respectively. The fruiting body characteristics of each variety were observed and recorded, including the following:

[0104] 1. Observe and record the mycelial and sclerotial conditions at the contact points between the nutrient bags and the soil for each strain after 30 days of storage, as well as the intensity of the white mycelial bloom on the soil surface. For each strain, three points were randomly selected, with 50 nutrient bags at each point, for a total of 150 bags. Observe and record the number of nutrient bags infected with contaminating microorganisms and calculate the contamination rate.

[0105] (See Table 5)

[0106] Table 5. Contamination rate of strains

[0107]

[0108] Table 5 shows that after 30 days in the nutrient bags, the mycelium of control group 6 (Six Sister K) was the yellowest and most concentrated on the soil surface, with a large number of sclerotia forming, and the lowest contamination rate. This was followed by control group 7 (Seven Sister), and then control group 6 (Six Sister). This indicates that control group 6 (Six Sister K) performed better than the other two controls in absorbing nutrients from the bags, exhibiting a large and dense mycelium, strong vitality, and strong resistance to contamination. The mycelial bloom of control group 6 (Six Sister K) was significantly whiter than the controls, and its mycelium had strong penetrating power. A prominent characteristic of control group 6 (Six Sister K) is its extremely white mycelial bloom, meaning that the mycelium can produce a large number of white conidia on the uncovered soil surface. The contamination rate of control group 6 (Six Sister K) was the lowest at 1%, followed by control group 7 (Seven Sister 2) at 12%, and finally control group 6 (Six Sister 1) at 21.5%, proving that control group 6 (Six Sister K) had vigorous mycelial vitality and the strongest resistance to contamination. (See appendix) Figure 8 , attached Figure 9 )

[0109] 2. Test the absorption rate of nutrient bags for each variety. After 45 days of storage, randomly select 20 nutrient bags for each variety to calculate the nutrient bag absorption conversion rate.

[0110] Table 6. Conversion rate of each strain in nutrient bags after 45 days.

[0111]

[0112] Table 6 shows the nutrient bag conversion rate of each strain after 45 days. The highest conversion rate was achieved by strain 6 (51.04%), followed by strain 7 (50.65%) (control 2); the lowest was strain 6 (44.79%) (control 1). This indicates that strain 6 (51.04%) has a superior ability to decompose and absorb nutrients from the nutrient bag, and its advantage over the control strain 6 (51.04%) is more significant.

[0113] 3. Growth of each strain under different pH conditions in nutrient bags: The pH was adjusted using lime and citric acid. Twenty nutrient bags were designed for each pH gradient, as detailed in the table below. The nutrient bags were placed at the designated time, and the mycelial growth of each strain at each pH gradient was observed and recorded. See Table 7 for details.

[0114] Table 7. Growth of strains at different pH values

[0115]

[0116]

[0117] Experiments showed that strain 6mei K could grow under different pH conditions in nutrient bags, exhibiting a wider pH tolerance range, including at pH 4, with an optimal pH range between 5 and 8. This demonstrates a broader pH tolerance range compared to the control strain 6mei K.

[0118] 4. Observe and record the number of days from sowing to budding for each strain: The budding time for strain 'Six Sister K' was 30 days; for strain 'Six Sister Control 1', it was 33 days; and for strain 'Seven Sister Control 2', it was 38 days. This indicates that under the same conditions, strain 'Six Sister K' produced fruit earliest. (Note: The budding time varies in different regions and under different climatic conditions.)

[0119] 5. Observe and record the morphological characteristics of the fruiting body development stages of each variety. The growth and development of the fruiting body is usually divided into four stages: the primordia stage, the young mushroom stage, the growth stage, and the maturity stage. (See appendix) Figure 10 ,11,12)

[0120] The experiment showed that during the primordial stage, both Sister K and Sister Control 2 did not undergo a large-scale formation of fish-egg-shaped primordia; instead, they directly began differentiating into pinhead-shaped mushrooms. In Sister Control 1, during the primordial stage, a large number of fish-egg-shaped primordia were formed first, and then pinhead-shaped mushrooms were differentiated on the fish-egg-shaped primordia. During the primordial stage, Sister K was whiter, while Sister Control 2 was darker.

[0121] Young mushroom stage: The morphological characteristics of Liu Mei K, Liu Mei control 1 and Qi Mei control 2 are quite similar at this stage, all with small black heads and white stems.

[0122] Growth period: Six Sister K has a yellowish cap, a particularly long and thin cap, fine and dense cap texture, a pointed cap tip, and a white and relatively thin stem; Six Sister Control 1 has a reddish cap, wider cap texture, a more pointed cap tip, and a white and relatively thick stem; Seven Sister Control 2 has a plump and round cap, a color similar to the tiered variety, wider texture, a round and pointed cap tip, and a white and the smallest stem.

[0123] Maturity: Six Sister K has a black cap, is slender, medium-sized, pointed, with fine veins, and a short, thin, white stem. The cap-to-stem ratio is mostly 3:1. Six Sister Control 1 has a black cap, is large, pointed, with wide veins, and a long, thick, white stem. The cap-to-stem ratio is mostly 2:1. Seven Sister Control 2 has a black cap, is medium-sized, round and pointed, with wide veins, and a short, thin, white stem. The cap-to-stem ratio is mostly 3:1.

[0124] Through observation and comparison, we can clearly see that, except for the young mushroom stage, which is similar to both Liu Mei K and Liu Mei Control 1 and Qi Mei Control 2, Liu Mei K shows significant differences from the control varieties in other growth and development stages. Furthermore, Liu Mei K has a superior fruiting body shape.

[0125] 6. Randomly select 50 harvested fruiting bodies for weighing, and compare the weight of a single fruiting body; compare the yield of each variety.

[0126] Table 8. Single mushroom weight and yield of each strain

[0127] Sixth Sister K 31.5 8.7 0.58 Sixth Sister Comparison 1 40.7 7.05 0.47 Seven Sisters Comparison 2 28.3 6.75 0.45

[0128] Measurements showed that the average weight of a single mushroom of the "Six Sisters K" variety was typically between 20-50g, with an average weight of 31.5g, classifying it as a medium-sized mushroom. The average weight of a single mushroom of the "Six Sisters Control 1" variety was typically between 25-65g, with an average weight of 40.7g, classifying it as a medium-to-large-sized mushroom. The average weight of a single mushroom of the "Seven Sisters Control 2" variety was typically between 20-50g, with an average weight of 28.3g, classifying it as a medium-sized mushroom. "Six Sisters K" exhibited the highest yield in this experiment, with an average yield of 0.58kg per square meter, 23% higher than "Six Sisters Control 1" and 29% higher than "Seven Sisters Control 2," demonstrating a significant difference.

[0129] Example 5: Regional Production Cultivation Trial

[0130] From 2020 to 2021 and from 2021 to 2022, we conducted multi-site production trials of Liu Mei K in different regions for two consecutive cultivation seasons. The control strain used was Liu Mei Control 1, which had the largest cultivation and promotion area. The growth and yield of the test strains in different regions were analyzed. The experimental results are as follows:

[0131] 1) Regional production trials conducted from November 2020 to April 2021 are shown in Table 9;

[0132] Table 9 Growth of strains

[0133]

[0134] As shown in Table 9, from November 2020 to April 2021, we conducted regional production trials in three locations. The results showed that strain 'Liu Mei K' had a thick white mycelial bloom, strong mycelial vitality, better nutrient bag absorption than strain 'Liu Mei', a higher nutrient bag conversion rate, better stress resistance, and almost no infection in the later stages of nutrient bag production compared to strain 'Liu Mei'. The quality of 'Liu Mei K' commercial mushrooms was superior to that of strain 'Liu Mei 1' (control strain). There were some differences in yield between the two locations due to differences in management, facilities, climate, and soil. At the Xinjin test site, the low temperature caused high mortality of small 'Liu Mei' mushrooms, resulting in a significantly lower yield. However, strain 'Liu Mei K' withstood the test, demonstrating significantly stronger low-temperature resistance than strain 'Liu Mei 1'. At the Guanghan test site, the use of plastic greenhouses for insulation prevented the impact of low temperatures, resulting in comparable yields. At the Lixian test site, both strains performed well. Overall, the results indicate that strain 'Liu Mei K' has stronger adaptability to cultivation.

[0135] 2) Production trials from November 2021 to April 2022 are shown in Table 10;

[0136] Table 10. Growth of the strains

[0137]

[0138] As shown in Table 10, from November 2021 to April 2022, we conducted regional production trials in three locations. The results showed that strain 'Liu Mei K' had a thick white mycelial bloom, strong mycelial vitality, better nutrient bag absorption than 'Liu Mei', a higher nutrient bag conversion rate, and almost no infection in the later stages of nutrient bag production, demonstrating stronger resistance to contamination. The quality of 'Liu Mei K' commercial mushrooms was superior to that of the control strain 'Liu Mei 1'. Yields varied significantly across the three locations due to differences in climate and soil conditions, but 'Liu Mei K' consistently outperformed the control strain 'Liu Mei 1' in all three locations. In the Jintang trial site, 'Liu Mei K' yield reached 710 jin per mu, with excellent quality. In the Mianyang trial site, 'Liu Mei' formed a large number of primordia in the early stages, but was affected by low temperatures before the Lunar New Year, resulting in widespread yield reduction and near-total crop failure. 'Liu Mei K' performed well in the Mianyang trial site; primordia formation did not occur as early as the control strain 'Liu Mei 1', but began when the temperature was suitable, avoiding the low temperatures and starting to produce mushrooms. However, the growth period was disrupted by more than ten consecutive days of high temperatures, causing some young mushrooms to die, resulting in a relatively low yield. Production trials in various regions have shown that Liu Mei K is superior to Liu Mei Control 1 in both low-temperature and high-temperature tolerance, exhibiting better stress resistance. Overall, Liu Mei K demonstrates stronger adaptability to cultivation.

[0139] Example 8: Comparative Analysis of the Quality of Commercial Mushrooms from Strains 6-Sister K and 6-Sister Control 1

[0140] The cap of fresh mushroom "Six Sister K" is darker in color than "Six Sister," and its cap is longer and more pointed than "Six Sister Control 1." The texture of "Six Sister K" is finer, while "Six Sister Control 1" has wider textures. The stem is whiter. In terms of flesh, "Six Sister Control 1" is slightly thicker than "Six Sister K." Fresh mushrooms from "Six Sister K" crumble less than those from "Six Sister Control 1." Overall, the quality of fresh mushrooms from "Six Sister K" is better than that from "Six Sister Control 1." (See attached image) Figure 15 )

[0141] The dried mushrooms from Liu Mei K are darker in color and have narrower veins than Liu Mei Control 1. Currently, black color and fine veins are more favored in the dried mushroom market. Overall, the dried mushrooms from Liu Mei K are darker, more pointed, and longer, making their appearance superior to Liu Mei Control 1 in every aspect. (See attached image) Figure 16 )

[0142] Finally, it should be noted that the above specific embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to examples, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications and substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A type of morel mushroom ( Morchella sextelata Strain 6-Sister K, characterized in that, This strain was deposited at the China Center for Type Culture Collection on October 25, 2022, with accession number CCTCC NO: M 20221647.

2. Fruiting bodies, mycelia, or spores produced by the morel strain 6mei K as described in claim 1.

3. The sub-entity according to claim 2, characterized in that, The sub-entities can be single or clustered.

4. The sub-entity according to claim 2, characterized in that, The fruiting body is shaped like a sheep's stomach, with a white, hollow stem and the rest being black.

5. The sub-entity according to claim 2, characterized in that, The fruiting body has an elongated conical cap.

6. The method for cultivating the morel strain 6mei K according to claim 1, characterized in that, Inoculate the strain into PDA medium and incubate at 20-25°C.

7. The cultivation method according to claim 6, characterized in that, The PDA culture medium comprises potato at a final concentration of 100-200 g / L, glucose at a final concentration of 10-50 g / L, and agar at a final concentration of 20-50 g / L.

8. Use of the fruiting bodies and / or mycelia produced by the morel strain 6mei K as described in claim 2 in food processing.

Images