Method for processing starch wastewater as well as product and application thereof
A treatment method and technology for starch wastewater, applied in the field of environmental engineering, can solve the problems that starch manufacturers are difficult to bear, difficult to treat starch wastewater, and high process costs, and achieve the effect of resource treatment.
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Embodiment 1
[0036] 1. Preparation of mold seed solution
[0037] (1) Strain resurrection and expanded cultivation: Take Aspergillus niger, Rhizopus taiwanensis and Aspergillus oryzae strains out of the refrigerator, and inoculate them into their respective sterilized resurrection solid medium by ultra-clean bench operation, and place them in the incubator Incubate at 28°C for 3 days to revive the bacteria. The revived strains were inoculated on respective revived plate medium for expansion culture, and cultured in an incubator at 28°C for 5 days, so that the growth potential of the bacteria was the most vigorous, and colonies were formed.
[0038] (2) Seed liquid preparation: Pick a small amount of Aspergillus niger, Aspergillus oryzae and Rhizopus formosanus from the plates after the expanded culture and inoculate them into 30mL modified starch Martin medium, culture in a shaker at 28°C and 150rpm for 24h Finally, the absorbance value at 560nm was detected by a UV-visible spectrophotome...
Embodiment 2
[0045] Get the prepared Aspergillus niger seed liquid of 5.2mL embodiment 1, inoculate in 130mL cassava starch wastewater, total CODcr12335.1mg / L in this wastewater, total BOD 5 6789.2mg / L, SS8.4g / L, pH4.0. The initial pH value of the wastewater was adjusted to 5.0, the stirring speed was 180 rpm, and the fermentation was carried out at 29.5° C. for 24 hours. Continue to inoculate 5.2 mL of the Geotrichum candidum seed liquid prepared in Example 1, the stirring speed is 180 rpm, the pH value of the solution is 5.5, and ferment at 29.5° C. for 2 days. The CODcr value of wastewater was reduced by 86.6% by measuring the chemical oxygen demand CODcr (national standard GB11914-89) of the water quality by the dichromate method. The treated wastewater was centrifuged at 8000 rpm for 30 minutes to collect the precipitated bacteria, and the dry weight of the bacteria was measured. After the bacteria were frozen and thawed three times, the cells of the bacteria were disrupted in an ul...
Embodiment 3
[0047] Get the prepared Aspergillus niger seed liquid of 6mL embodiment 1, inoculate in 130mL cassava starch waste water, total CODcr12430.1mg / L in this waste water, total BOD 5 7695.4mg / L, SS9.6g / L, pH3.9. The initial pH value of the wastewater was adjusted to 5.5, the stirring speed was 200 rpm, and fermentation was carried out at 29.5°C for 24 hours. Continue to inoculate 6 mL of Rhodotorula rubrum seed solution prepared in Example 1, stirring at 200 rpm, pH of the solution at 6.5, and ferment at 29.5° C. for 2 days. The CODcr value of wastewater was reduced by 76.9% by measuring the chemical oxygen demand CODcr (national standard GB11914-89) of the water quality by the dichromate method. The treated wastewater was centrifuged at 8000 rpm for 30 minutes to collect the precipitated bacteria, and the dry weight of the bacteria was measured. After the bacteria were frozen and thawed three times, the cells of the bacteria were disrupted in an ultrasonic pulverizer (power 380W...
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