Method for producing vitamin K2 by utilizing bacillus natto

A technology of Bacillus natto and vitamins, which is applied in the field of producing vitamin K2 by using Bacillus natto, and can solve problems such as increasing MK production

Inactive Publication Date: 2012-12-05
上海红马饲料有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Yeast extract is not suitable as a nitrogen source alone, but after additional n

Method used

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Embodiment

[0038] Rejuvenation of strains: After the rejuvenation of strains BS-5 and CICC10262, they were inoculated on a slant solid medium, cultured at a constant temperature of 37°C for 18-24h, and stored at 4°C.

[0039] Seed culture: The activated strains were put into the liquid medium (30 / 250ml), and cultured at 37°C, 120r / min for 48h. Take the seed culture solution, insert it into the liquid medium (30 / 250ml) according to the inoculum amount (v / v) of 5%, and cultivate at a constant temperature of 37°C and 120r / min.

[0040] Ultraviolet mutagenesis of BS-5 bacterial strain: the bacterial suspension of BS-5 bacteria in the early logarithmic growth period was centrifuged at 3500R / min to collect the bacterial cells, and after washing the bacterial cells with sterilized physiological saline for 2-3 times, the cell concentration was made into 1×108 / mL bacterial suspension.

[0041] On the ultra-clean workbench, draw 15 mL of the bacterial suspension into a sterile petri dish with a d...

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PUM

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Abstract

The invention aims to disclose a method for producing vitamin K2 by utilizing bacillus natto. Protoplast fusion is conducted on bacillus natto BS-53 highly yielding the vitamin K2 and bacillus natto CICC10262 having a high growth speed but a low vitamin K content, and fusants with parent advantages are screened out; obtained high-yield strains having a high growth speed are utilized to produce the vitamin K2 under an optimized fermentation condition; and isopropanol and n-hexane are utilized to extract and separate the vitamin K2. The growth speed of the strains and the yield of the vitamin K2 is increased and an effective method is provided for scale production of the vitamin K2, thereby realizing the aim of the invention.

Description

technical field [0001] The invention relates to a method for producing vitamin K 2 The method for, particularly relates to a kind of utilization bacillus natto to produce vitamin K 2 Methods. Background technique [0002] Vitamin K (MK) is a general term for a class of anti-hemorrhagic compounds containing a formazan structure, also known as coagulation vitamins or anti-bleeding vitamins. Vitamin K (MK) can be divided into two categories, one is the fat-soluble compound K 1 、K 2 and K 3 , which can be extracted in animals and plants; the other is water-soluble compound K 4 , artificial chemical synthesis. The most important is K 1 、K 2 and K 3 , vitamin K for commercial use is vitamin K 3 Derivatives. In animals, the biological activity is K 2 , while vitamin K 1 and K 3 needs to be converted to K in the liver 2 to function. [0003] Vitamin K 2 Not only in the process of blood coagulation, but also in bone metabolism, it has been widely used in the preventi...

Claims

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Application Information

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IPC IPC(8): C12P7/66C12R1/07
Inventor 刘国庆赵肖胡卫华钱晓勇
Owner 上海红马饲料有限公司
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