262 results about "Vitamin L2" patented technology

The present invention is directed to compositions comprising a nanoparticulate vitamin K2 having improved bioavailability. The nanoparticulate vitamin K2 particles of the composition have an effective average particle size of less than about 2000 nm and are useful in the prevention and treatment of osteoporosis. The invention also relates to a controlled release composition comprising a vitamin K2 or a nanoparticulate vitamin K2 that in operation delivers the drug in a pulsed or multi-modal manner for the prevention and treatment of osteoporosis.

The invention discloses a vitamin K2 and preparation process thereof. According to the process, 1-hydroxyl-2-naphthoic acid resistant flavobacterium strain HNA12-D is fermented and cultured, and vitamin K2 efficiently metabolizes in the strain through the batch feeding of glycerin serving as the carbon source, peptone serving as the nitrogen source and precursor substances, and is extracted by using organic solvents and separated by macroporous resin, and the yield and purity of the product are improved. The total output of the vitamin K2 crude product is 200-600 mg/L after the flavobacterium strain HNA12-D of the invention is fermented and cultured for 120-144 hours, and the product purity reaches 80-99 percent after absorption chromatography; compared with the prior art, the yield output and the product purity are greatly improved, and the product can be used in industrial production.

The invention aims to disclose a method for producing vitamin K2 by utilizing bacillus natto. Protoplast fusion is conducted on bacillus natto BS-53 highly yielding the vitamin K2 and bacillus natto CICC10262 having a high growth speed but a low vitamin K content, and fusants with parent advantages are screened out; obtained high-yield strains having a high growth speed are utilized to produce the vitamin K2 under an optimized fermentation condition; and isopropanol and n-hexane are utilized to extract and separate the vitamin K2. The growth speed of the strains and the yield of the vitamin K2 is increased and an effective method is provided for scale production of the vitamin K2, thereby realizing the aim of the invention.

The invention discloses a method for improving output of methane produced by anaerobic digestion of inferior biomass raw materials, and belongs to the technical field of agricultural waste treatment. According to the method, various waste inferior biomass raw materials is used as a processing object, trace of electronic carrier hemin or vitamin K2 is added into an anaerobic reactor to improve the reducing force level of the whole fermentation system of biogas anaerobic fermentation so as to promote the growth and activity of hydrolytic acidification bacteria and methanogenic bacteria in the anaerobic digestion process, and the hydrolysis efficiency of the reaction system is improved, the anaerobic digestion time is shortened, and the yield of the methane and the conversion rate of the inferior biomass raw materials are improved. Due to the addition of extremely trace hemin and vitamin K2, industrial grade hemin and vitamin K2 are also applicable to the method, and the method has the advantages of simple operation and high efficiency and low cost, and is expected to be widely used in engineering practice.

The present invention provides Natto manufactured by mixing steamed soybean, cooked barley and yam, inoculating Bacillus subtilis Natto to the mixture, and fermenting the mixture, which is almost odorless, soft, good taste, and viscous, and further, functional substances such as vitamin K2 and enzyme (Nattokinase and Protease) are remarkably increased. This product may extend the utilization as functional traditional foods relating to prevention of life-style related disease.

A Bacillus natto culture is treated with chitosan, and then filtered, concentrated, and dried. According to this method, a Bacillus natto culture extract containing nattokinase and 1 μg or less of vitamin K2/g dry weight is obtained.

The invention discloses a method for simultaneously producing vitamin K2 and nattokinase through Bacillus natto fermentation. The method comprises the following steps: strain activation: performing streak culture on Bacillus natto in a plating culture medium, and culturing a single colony in a test tube culture medium; seed preparation: inoculating the Bacillus natto in the test tube into a seed culture medium, and culturing; production of nattokinase and vitamin K2: preparing fermentation culture medium for simultaneous production, taking the prepared seed solution after the fermentation culture medium for simultaneous production is cooled, inoculating, and producing the nattokinase and vitamin K2 under optimal culture conditions for simultaneous production; and separation and extraction of the nattokinase and vitamin K2: centrifuging the fermentation solution, separating the supernatant from the precipitate, adding inorganic high polymer flocculant into the supernatant, and taking chitosan as coagulant aid to separate the vitamin K2 and nattokinase in the supernatant. The method has the advantages of improving the raw material utilization ratio and being suitable for industrial production.

Disclosed are solid forms of menaquinol and processes for obtaining them by chemical or enzymatic reduction of menaquinone. Said solid forms possess high stability to oxidation which allows effective use of menaquinol in the most common formulations wherein vitamin K2 is used.

The invention discloses bacillus subtilis natto for high yield rate of vitamin K2 (MK-7) and application thereof, and belongs to the field of microorganisms. The bacillus subtilis natto ND-1-A27 was collected into China Center for Type Culture Collection on March 14, 2018 in Wuhan University of China, and the collection number is CCTCC NO:M 2018131. The bacillus subtilis natto has the advantages that the yield rate of the vitamin K2 (MK-7) is greatly increased and is about 70mg/L, and the maximum yield rate can be reached by about three days; the fermenting cycle is greatly shortened, the timeis shortened, and the cost is reduced; the bacillus subtilis natto can be applied to industrialized fermenting production, and the genetic stability is good; after five generations are continuously transferred, the yield rate of the vitamin K2 (MK-7) is basically stable, and is maintained at the higher level; the bacillus subtilis natto can be used as a production bacterial strain for further study and development.

The invention belongs to the field of health care food, and especially relates to a composition used for improving skeletal system health, and applications thereof. The composition comprises skimmed milk powder, fructo-oligosaccharide, yak bone, orange fruit powder, D-glucosamine hydrochloride, bone collagen, chondroitin sulfate, herba epimedii extract, rhizoma drynariae extract, mushroom powder, magnesium carbonate, casein phosphopeptide, and vitamin K2. The composition possesses excellent prevention effects on patients with osteoporosis and skeletal system disorders such as ankylosis, arthritis, osteoproliferation, arthralgia, and myospasm, and is capable of increasing bone mineral density, and improving skeletal system health. The composition can be used for preparing health care food capable of improving skeletal system health; application range is wide; and economic benefit and social benefit are both high.

This invention discloses a method for synthesizing vitamin K2 product. The method comprises: reducing 2-methyl-1, 4-naphthoquinone into hydroquinone by using KBH4, performing Fridel-Crafts alkylation reaction with long-chain tert-terpenol in nitromethane/n-hexane system with anhydrous ZnCl2, boron trifluoride diethyl etherate and CuBr as the catalysts to introduce long-chain terpenyl to the C3 site of hydroquinone, oxidizing with isopropanol solution of FeCl3 to obtain corresponding vitamin K2. The method has such advantages as mild reaction conditions, high yield, recoverable reagents, and no environmental pollution.

The invention relates to prodrugs of vitamin K2, in particular prodrugs of MK-7 in which the diketone is converted to a monosubstituted or disubstituted ester type derivative. These compounds are shown to give MK-7 in plasma.

The invention belongs to the field of dairy products, and particularly relates to child milk powder for promoting bone growth and a preparation method thereof. The milk powder comprises the followingcomponents in parts by weight: 3000-4000 parts of raw cow milk or raw goat milk, 100-200 parts of skim milk powder, 100-200 parts of desalted whey powder, 30-80 parts of composite vegetable oil or 1,3-dioleic acid-2-palmitic acid triglyceride, 50-150 parts of solid corn syrup, 50-150 parts of whey protein powder, 30-100 parts of crystalline fructose, 20-50 parts of fructo-oligosaccharide, 10-30 parts of anhydrous cream, 5-20 parts of galacto-oligosaccharide, 1-3 parts of compound vitamin, 1-3 parts of compound mineral and 2.2-9 parts of a calcium promoting component. By adding casein phosphopeptides, hydrolyzed yolk powder, vitamin K2 powder and other components, the calcium content is increased, and the absorption and utilization rate of calcium can be effectively increased; and by combining with probiotics, dietary fibers and other nutritional ingredients, the bone growth of children can be effectively promoted, the immunity is improved, and the growth requirements of the children are met.

The invention provides a vitamin K2 compound for preventing and treating arthritis, and belongs to the field of medicine or health-care food formulae. Per 1000g of the compound comprises 0.05-0.25g of vitamin K2, 0.005-0.01g of vitamin D, 500-750g of glucosamine and 499.945-249.740g of solid or liquid matrix. The compound which is mechanically stirred and uniformly mixed under a normal temperature or heating condition is prepared to soft capsules, hard capsules or troches. The medicine or health-care food formed by the formula has remarkable preventing and treating effects on common osteoarthritis of the middle aged or the aged people, especially postmenopausal women.

The invention relates to a strain of bacillus amyloliquefaciens capable of generating a great amount of MK-7 (menadione-7) in a culture process and a culture method thereof. The name of the strain is Y-2, and the class name is bacillus amyloliquefaciens. The obtained strain has stable hereditary character and stable yield trait, is suitable for industrial popularization, and is collected in the China Center for Type Culture Collection (CCTCC), with the collection number CCTCC No.M2013493. The invention also discloses a method for producing MK-7, which comprises the following steps: with 5% of seed inoculation amount, inoculating a seed solution into a fermentation medium; culturing for 2 hours at a rotation speed of 100r/min and a temperature of 37 DEG C; performing stationary culture for 6 days, wherein the highest yield of vitamin K2 (MK-7) reaches 66.62mg/L. According to the MK-7 high-yielding strain and production method provided by the invention, the production cycle is short, the yield is high, and feasibility is provided to the production of MK-7 through industrial fermentation.

The invention relates a preparation containing organic effective components and having curative and healthy functions on osteoporosis and cardiovascular and cerebrovascular diseases. The preparation is prepared by mixing vitamin K2 0.01 to 0.5 g with coenzyme Q10 5 to 10 g, heating with walnut oil 300 g at 40 DEG C, stirring to dissolve, adding walnut oil 599.5 to 694.99 g under stirring, and mixing uniformly to obtain a mixed solution 1000 g. The solution can be further packaged to soft capsule or hard capsule, and the vitamin K2 is one or a plurality of kinds of vitamin K2 (5) to K2 (50). The preparation can be used for prevention and assistant treatment of the cardiovascular disease, and has the healthy and the curative effects on increasing bone density and resisting osteoporosis.

The invention aims to disclose a vitamin K2 high-yielding strain. Compared with the prior art, bacillus natto BS-5 is taken as an original strain, after the ultraviolet and nitroso arc composite mutation, the mutant strain BS-53 is obtained after screening, the mutant strain BS-53 and bacillus natto CICC (China center of industrial culture collection)10262 which has low vitamin K2 yield but high growth rate, are subjected to protoplast fusion, fusant is obtained through the screening of a flat screen, and the fusant is finally obtained after the selecting of the fermenting property experiment; and the vitamin K2 high-yielding strain has excellent characteristics of parents, i.e. has the dual effects of high MK (midkine) yield and high growth rate, an effective method is provided for realizing high MK yield, important scientific meaning and application value are obtained, and the purpose of the invention is realized.

Vitamin K2 submicron emulsion is prepared from, by weight, 0.0005-1% of vitamin K2, 1.0-10% of oil, 0.1-1.2% of phospholipid, 0.5-2.3% of emulgator, 0.5-3.0% of antifreezing agent and water according to the total weight of the vitamin K2 submicron, wherein emulgators except phospholipid can serve as the emulgator in the emulsion. The vitamin K2 submicron emulsion is good in sterilization stability and freeze-thaw stability, and the expected shelf life can reach two years or more.

The invention discloses a method for measuring content of vitamin K2 (MK-7) and mainly relates to the sample extraction technology and chromatographic separation technology of the vitamin K2 (MK-7). The content measuring method comprises the following steps: (1) preparing a sample solution; (2) measuring the content of the vitamin K2 (MK-7), namely measuring the sample solution by utilizing a high performance liquid chromatography technology to obtain the peak area of a chromatographic peak of the vitamin K2 (MK-7), calculating the content of the vitamin K2 (MK-7) in a tested sample according to a standard curve, and calculating in combination with the mass of the sample to obtain the content of the vitamin K2 (MK-7). The method can be used for rapidly measuring the content of the vitamin K2 (MK-7) contained in health foods, medicines, common foods or premix, the quality of the health foods, medicines, common foods or premix containing the vitamin K2 (MK-7) is evaluated, and the content measuring method is accurate, rapid, convenient and effective.

The invention discloses an arthralgia relief composition, an arthralgia relief health-care product, and application of the arthralgia relief composition. The arthralgia relief composition comprises D-glucosamine sulfate potassium chloride, calcium carbonate, chondroitin sulfate, type II collagen, non-denatured type II collagen, turmeric extract, vitamin K2 powder, and vitamin D3 powder. Human feeding trials prove that the components are synergistic, allowing arthralgia to be relieved shortly, promoting joint activity improvement, and preventing further degenerative changes in articular cartilages; the arthralgia relief composition has good safety and effect, and a quick, effective scheme is provided for relief of osteoarthritis or rheumatoid arthritis pains.

An anti-aging composition comprising: pinebark extract, polypodium leucotomos, ashwagandha extract, dehydroepiandrosterone, white tea extract, alpha lipoic acid, vitamin K2, bioactive silicon, hyaluronic acid, trimethylglycine, and optionally, piperine, green tea extract, folic acid, vitamin C, camu camu, and blueberry extract.

The invention discloses an organic fertilizer formula for significantly improving fertilizer utilization rate. The organic fertilizer formula comprises, by weight, 5-10 parts of soybean oligosaccharide, 84.99-92.995 parts of an organic material containing 16% fulvic acid, 2-5 parts of a 5% polyglutamic acid liquid agent, and 0.005-0.01 parts of vitamin K2. With the present invention, the problems of soil hardening, reduction of the yield increasing effect of the fertilizer, agricultural product quality decreasing, environmental pollution, and the like due to excessive chemical fertilizer utilization are effectively solved; the organic fertilizer of the present invention has characteristics of stable performance, simpleness and feasibility, and low cost, and is applicable for popularization and application in a large area; the agricultural sustainable development can be effectively promoted.