Continuous culture system and method for human intestinal canal flora

Abstract

The invention relates to a continuous culture system for human intestinal flora and a method thereof. The system includes a set of fermentor, a culture medium input system, a waste liquid output system, a liquid level control system and a gas-path system. The invention establishes a continuous culture system for human intestinal flora and a method thereof, and builds a vitro model simulating human intestinal environment. The continuous culture system is controlled by a computer and the model has the advantages of simple operation, convenient usage and good system stability. The results indicate that the human intestinal flora can grow stably in the system, and the amount of predominant bacteria and the concentration of major metabolites (SCFA) are the same as that of a healthy person. The establishment of the model not only builds a platform for safety evaluation of antibacterial residue effecting human intestinal flora, but also provides more scientific method and way for safety evaluation research of new drug, functional food and the like.

Description

technical field [0001] The invention belongs to the technical field of microbiology and the technical field of veterinary drug residue analysis, and in particular relates to a continuous culture system and method for human intestinal flora. Background technique [0002] The human gastrointestinal tract is populated by a very complex and active microbial community, including about 10 14 bacteria, 10 per gram of intestinal contents 11 There are about 400-500 species of bacteria, and their interactions are extremely complex. A large number of studies have shown that this huge microbial flora achieves a balanced state in the human intestinal tract, which is crucial to human health and disease conditions. While inhibiting the growth of pathogenic bacteria, antibacterial drugs can also disrupt the balance of intestinal flora and cause superinfection. In recent years, studies have found that antimicrobials remaining in animal foods can have adverse effects on human intestinal fl...

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Problems solved by technology

[0005] At present, there is only one patent document closely related to the present invention in China, which is a patent application with the application number 200710028346.2 (an in vitro culture device for intestinal flora) published by the State Intellectual Property Office of China on January 30, 2008 , the application used a self-built fermenter and other accessories to construct a continuous culture device for intestinal flora. The purpose of the invention is to provide an in vitro culture device for intestinal flora that simulates the intestinal environment, and its advantages It has successfully established a chemostat model for the continuous culture of human intestinal flora, but judging from the published instructions and claims, the application did not provide relevant biological experimental data that fully explained the effect of the invention, and it was impossible for people to understand the effects of the invention. The effect of the implementation, and there is no example to illustrate the specific construction method of the model, that is, the specific continuous culture method of the flora. There are only simple operation instructions for the operation of the device, and it is difficult to really popularize the model. In addition, the device still has a system that is not stable enough , Routine maintenance is relatively complicated, conditions are not easy to control, etc.

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