CAR-T cell for treating AIDS-associated lymphoma, and preparation method and application thereof

A tumor cell and cell technology, applied in the direction of genetically modified cells, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of missed treatment timing, long process, low tolerance, etc., to reduce the risk of GVHD , strong amplification and differentiation capabilities, simple collection process
CN107904259APending Publication Date: 2018-04-13SHANGHAI LONGYAO BIOTECH CO LTD
3 Cites 27 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI LONGYAO BIOTECH CO LTD
Publication Date
2018-04-13

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention discloses a preparation method of a CAR-T cell for treating AIDS-related lymphoma. A CD8+ T cell is used to produce the CAR-T cell, and the CD8-T cell derives from a cord blood T cell. The method includes the following steps: preparing cord blood mononuclear cells from cord blood, removing tumors and other cells by using a human T cell purification kit to obtain T cells, and carryingout separation by using magnetic beads to obtain the CD8+ T cell; activating the CD8+ T cell by using an appropriate medium and appropriate stimulation conditions; transferring CAR to the CD8+ T cellby using lentivirus to prepare the CAR-T cell; and amplifying the CAR-T cell in vitro by using cytokines and activating stimulators to achieve the desired effective dose. The invention also relates to the CAR-T cell prepared by the method, and an application thereof. The CAR-T cell prepared in the invention has a good cell activity and a high proliferation speed, and reduces the attack risk of GVHD; and only the CD8+T cell of the umbilical blood T cells of AIDS patients is used to prepare the CAR-T cell for, so the risk of input CAR-T infected with in-vivo HIV is reduced.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to the technical field of cellular immunotherapy, in particular to a CAR-T cell for treating AIDS combined with lymphoma and its preparation method and application. A method for preparing complex antigen receptor T cells (CAR-T) and cell preparations thereof. Background technique

[0002] The use of immunological treatment to overcome tumors has always been an important direction for the application of immunology in translational medicine. With the development of various omics (genomics, proteomics, etc.), the immunogenicity of tumor cells due to mutations has been widely recognized, which has laid a theoretical foundation for tumor immunotherapy. At the same time, with the accumulation of tumor immunology research itself, tumor immunotherapy has made great progress recently, and a series of new immunotherapy methods have gradually entered the clinic. The current tumor immunology research has established the central posi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More