Primers, probe and kit for detecting human MET gene 14 exon splicing mutation

Abstract

The invention discloses primers, a probe and a kit for detecting human MET gene 14 exon splicing mutation. The invention provides a specific primer pair A. The specific primer pair A is composed of a primer 1 and a primer 2 which are respectively disclosed as Sequence 3 and Sequence 4 in the sequence table. The invention also provides a primer-probe combination A which is composed of the specific primer pair A and a probe A, wherein the nucleotide sequence of the probe A is disclosed as Sequence 5 in the sequence table. The invention also provides a kit B which comprises the primer-probe combination A. The invention also provides application of the primer-probe combination A or the kit B in detecting whether mutation for causing MET gene 14 exon jump deletion occurs in the human genome DNA (deoxyribonucleic acid). The method is simple to operate, has the advantages of short detection time, high sensitivity, high specificity, high safety, no toxicity, low cost and the like, and can satisfy the actual demands for clinical quick detection.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and clinical molecular diagnosis, and relates to a primer, a probe and a kit for detecting the splicing mutation of exon 14 of human MET gene. Background technique [0002] Lung cancer is the most common malignant tumor in the world, and its morbidity and mortality rank first among all cancers. 80%-85% of lung cancer cases are non-small cell lung cancer (NSCLC). Every year, more than 1.3 million patients die of lung cancer worldwide, nearly half of which occur in developing countries. In recent years, the morbidity and mortality of lung cancer in China have shown a significant upward trend. The 5-year survival rate of lung cancer patients in my country is only 13%. The main reason is that most lung cancers lack highly sensitive gene mutation detection and diagnosis techniques. , and the matching scientific treatment plan, so that patients miss the best time for treatment. [0003] At presen...

AI Technical Summary

Problems solved by technology

[0005] At present, there is no commercial kit for the detection of MET14 exon splicing mutations. A few research institutions use high-throughput sequencing to scan the entire exon to detect the presence of MET14 exon splicing mutations. This method has high sensitivity and can Simultaneously detect dozens or even hundreds of tumor-related genes, but the operation is complicated, the later data processing is cumbersome, the detection cycle is long and the detection cost is expensive, the requirements for operation and interpretation technology are very high, and there are still many deficiencies in clinical practice. For the above reasons, high-throughput sequencing is not suitable for large-scale screening and diagnosis of MET mutation-positive NSCLC patients at this stage

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