Tissue microarray of decidua and villi pairing, preparation method and application thereof

Abstract

The invention relates to the technical field of biology, in particular to a tissue microarray of decidua and villi pairing, a preparation method and application thereof. The tissue microarray is prepared with the decidua and villus specimens of a normal pregnancy woman or a pathologic pregnancy patient arranged in sample application holes, so that pairing is formed between the decidua tissue and the villus tissue from the same specimen, and the functional presentation of the maternal-fetal interface of a physiological structure onto the tissue microarray is achieved. The tissue microarray has the advantages that high throughput is achieved, plenty of information is obtained in one time, and the efficiency is improved by hundreds of times; parallelization is achieved, the comparability is high, and the precision is high; the experimental error is small, and experimental conditions remain the same; convenience is brought to arrangement of various kinds of experimental control, and the research cost is greatly reduced. The tissue microarray can be used for etiology screening and molecular typing of pathologic pregnancy, screening and verifying of a novel marker, screening of individual treatment patients, judgment of a therapeutic effect and prognosis and the like, and the tissue microarray has important significance for the research of a normal pregnancy maintaining mechanism and pathologic pregnancy pathogenesis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a paired tissue chip of decidua and villi, a preparation method and application thereof. Background technique [0002] Biochip refers to a high-throughput microarray of high-density tissues, cells, proteins, nucleic acids, sugars, and other biological components coated on solid supports such as silicon wafers and nylon membranes. Utilizing high-throughput biochips and specific molecular hybridization and staining techniques to detect the tested samples, a large amount of molecular detection information can be obtained accurately and quickly. At present, it has been widely used in the fields of life science research, molecular diagnosis of clinical medicine, and screening and verification of biological antibody-targeted drugs. Tissue chip technology can integrate hundreds of clinical tissue specimens from different individuals and different sources on a solid-phase carrier in a pre-d...

AI Technical Summary

Problems solved by technology

In-depth research on the formation of the maternal-fetal interface is an important way and means to reveal the maintenance mechanism of normal pregnancy and the pathogenesis of pathological pregnancy. Therefore, according to the characteristics of decidua and villi, a paired tissue chip of decidua and villi and its preparation method are provided. It is of great significance for the formation of the maternal-fetal interface, the screening of pathological pregnancy etiology, the screening and verification of new markers, the screening of patients with individualized treatment, and the judgment of curative effect and prognosis. However, the use of decidua and chorionic tissue to prepare tissue chips has not been found in the prior art technology and application reports

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