Tissue microarray of decidua and villi pairing, preparation method and application thereof

A tissue chip and villi technology, applied in the biological field, can solve the problems of undiscovered tissue chip technology and application reports, and achieve the effects of reducing research costs, small experimental errors, and improving efficiency

Active Publication Date: 2017-09-22
夷希微医学科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In-depth research on the formation of the maternal-fetal interface is an important way and means to reveal the maintenance mechanism of normal pregnancy and the pathogenesis of pathological pregnancy. Therefore, according to the characteristics of decidua and villi, a paired tissue chip of decidua and villi and its preparation method are provided. It is of great significance for the fo

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  • Tissue microarray of decidua and villi pairing, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of decidua and villi paired tissue chip (1)

[0036] (1) Collect decidua and villi tissues during curettage, and record the patient's age, BMI, number of abortions, treatment plan, endocrine, autoantibodies, blood coagulation, immunity, chromosome screening or genetic thrombophilia screening data parameters , to establish a database of clinical data. (2) Collect decidua and villi tissue samples, divide them into about 5mm×15mm×15mm in size, dehydrate the tissue samples, and embed them in paraffin wax, and select the samples with complete clinical data as the donor wax blocks. (3) Cut the donor wax block into 4-8 micron thick tissue sections with a fully automatic paraffin microtome. (4) The tissue sections were stained with HE by an automatic staining machine, and typical tissue areas were marked under a microscope. (5) Design each tissue chip to include 90 spotting holes, the spotting holes are arranged in an equidistant matrix on the paraffin s...

Embodiment 2

[0037] Example 2 Preparation of Decidua and Villi Paired Tissue Chip (2)

[0038] (1) Collect decidua and villi tissues during curettage, and record the patient's age, BMI, number of abortions, treatment plan, endocrine, autoantibodies, blood coagulation, immunity, chromosome screening or genetic thrombophilia screening data parameters , to establish a database of clinical data. (2) Collect decidua and villi tissue samples, divide them into about 5mm×15mm×15mm in size, dehydrate the tissue samples, and embed them in paraffin wax, and select the samples with complete clinical data as the donor wax blocks. (3) Cut the donor wax block into 4-8 micron thick tissue sections with a fully automatic paraffin microtome. (4) The tissue sections were stained with HE by an automatic staining machine, and typical tissue areas were marked under a microscope. (5) Design each tissue chip to include 160 spotting holes, the spotting holes are arranged in an equidistant matrix on the paraffin ...

Embodiment 3

[0039] Example 3 Preparation of Decidua and Villi Paired Tissue Chip (3)

[0040] (1) Collect decidua and villi tissues during curettage, and record the patient's age, BMI, number of abortions, treatment plan, endocrine, autoantibodies, blood coagulation, immunity, chromosome screening or genetic thrombophilia screening data parameters , to establish a database of clinical data. (2) Collect decidua and villi tissue samples, divide them into about 5mm×15mm×15mm in size, dehydrate the tissue samples, and embed them in paraffin wax, and select the samples with complete clinical data as the donor wax blocks. (3) Cut the donor wax block into 4-8 micron thick tissue sections with a fully automatic paraffin microtome. (4) The tissue sections were stained with HE by an automatic staining machine, and typical tissue areas were marked under a microscope. (5) Design each tissue chip to include 400 spotting holes, the spotting holes are arranged in an equidistant matrix on the paraffin ...

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Abstract

The invention relates to the technical field of biology, in particular to a tissue microarray of decidua and villi pairing, a preparation method and application thereof. The tissue microarray is prepared with the decidua and villus specimens of a normal pregnancy woman or a pathologic pregnancy patient arranged in sample application holes, so that pairing is formed between the decidua tissue and the villus tissue from the same specimen, and the functional presentation of the maternal-fetal interface of a physiological structure onto the tissue microarray is achieved. The tissue microarray has the advantages that high throughput is achieved, plenty of information is obtained in one time, and the efficiency is improved by hundreds of times; parallelization is achieved, the comparability is high, and the precision is high; the experimental error is small, and experimental conditions remain the same; convenience is brought to arrangement of various kinds of experimental control, and the research cost is greatly reduced. The tissue microarray can be used for etiology screening and molecular typing of pathologic pregnancy, screening and verifying of a novel marker, screening of individual treatment patients, judgment of a therapeutic effect and prognosis and the like, and the tissue microarray has important significance for the research of a normal pregnancy maintaining mechanism and pathologic pregnancy pathogenesis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a paired tissue chip of decidua and villi, a preparation method and application thereof. Background technique [0002] Biochip refers to a high-throughput microarray of high-density tissues, cells, proteins, nucleic acids, sugars, and other biological components coated on solid supports such as silicon wafers and nylon membranes. Utilizing high-throughput biochips and specific molecular hybridization and staining techniques to detect the tested samples, a large amount of molecular detection information can be obtained accurately and quickly. At present, it has been widely used in the fields of life science research, molecular diagnosis of clinical medicine, and screening and verification of biological antibody-targeted drugs. Tissue chip technology can integrate hundreds of clinical tissue specimens from different individuals and different sources on a solid-phase carrier in a pre-d...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/36
CPCG01N1/28G01N1/2813G01N1/36
Inventor 鲍时华王柳柳朱丹
Owner 夷希微医学科技(上海)有限公司
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