A Simple Isolation and Identification Method of Human Placental Myofibroblasts

A technology of fibroblasts and separation method is applied in the field of simple separation and identification of human placental myofibroblastic cells, which can solve the problems of complicated experimental steps, waste of resources and the like, and achieves convenient cell purification, short time-consuming and simple operation. Effect
CN103173405BActive Publication Date: 2017-11-07BEIJING JING MENG STEM CELL TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING JING MENG STEM CELL TECH
Publication Date
2017-11-07

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Abstract

The present invention relates to a method for separating and identifying human placental myofibroblasts. After the placental fetal decidual tissue was mechanically disrupted, it was digested with DMEM digested solution containing collagenase I, and isolated into mononuclear cells. Cultivate under humidity to induce adherence. After 48 hours, change the medium in full to remove non-adherent cells and add fresh medium. After adherent cells form monoclonal cells, each monoclonal cell is cultured separately. When the cells are about 90% confluent, Trypsin passage to obtain myofibroblasts. The invention adopts the combined method of cell morphology, cell cycle, cell surface marker detection, cell adipogenic and osteogenic differentiation ability detection, cell pluripotent stem cell factor detection and cell desmin expression detection to identify the obtained myofibroblasts.
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Description

field of invention

[0001] The invention relates to a method for isolating myofibroblasts from the decidua surface of human placenta and identifying the myofibroblasts. Background technique

[0002] The placenta is considered to be the most attractive "adult stem cell", which can differentiate into muscle cells, liver cells, osteoblasts, chondrocytes, adipocytes and other cells in a suitable in vivo or in vitro environment. Because the source of placental stem cells is relatively convenient, easy to separate and purify, it still has the characteristics of stem cells after multiple passages and expansion, and does not have the characteristics of immune rejection; therefore, it has become a hot spot in stem cell research in recent years. Transplantation, tissue engineering, genetic engineering and other fields have good application prospects.

[0003] Myofibroblasts refer to muscle tissue precursor cells containing myofilaments in the cytoplasm, derived from mesoderm stem cell...

Claims

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