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Method for obtaining maternal mesenchymal stem cells from placenta

A maternal-derived, mesenchymal stem cell technology, applied in cell dissociation methods, animal cells, vertebrate cells, etc., can solve the problems of loss of cell source, inability to obtain maternal-derived mesenchymal stem cells, fragmentation and other problems

Pending Publication Date: 2020-10-20
深圳华大基因细胞科技有限责任公司
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Problems solved by technology

The disadvantage of this method is that the number of maternal-derived mesenchymal stem cells obtained from the decidua is very small; and the decidua and amniotic membrane will be broken and broken during delivery, which is easy to lose, resulting in the loss of the source of cells and the inability to obtain maternal stem cells. Source mesenchymal stem cells

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  • Method for obtaining maternal mesenchymal stem cells from placenta
  • Method for obtaining maternal mesenchymal stem cells from placenta
  • Method for obtaining maternal mesenchymal stem cells from placenta

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Embodiment

[0060] 1. Main reagents and materials

[0061] Stem cell medium was purchased from Stempro, sodium chloride injection was purchased from Guizhou Tiandi, 50 mL centrifuge tubes, T75 culture flasks and T175 culture flasks were purchased from Corning, and collagenase, hyaluronidase, neutral protease and papain were purchased from Gibco.

[0062] Compound enzyme digestion solution: dissolve collagenase, hyaluronidase, neutral protease and papain in PBS solution to make a compound enzyme digestion solution; and, the concentration of collagenase in the compound enzyme digestion solution is 0.4% by mass fraction, The concentration of hyaluronidase is 0.1% by mass fraction, the concentration of neutral protease is 200U / mL, and the concentration of papain is 0.05% by mass fraction.

[0063] Control enzyme digestion solution: collagenase was dissolved in PBS solution to prepare a control enzyme digestion solution, wherein the concentration of collagenase was 0.5% by mass fraction.

[0...

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Abstract

The invention discloses a method for obtaining maternal mesenchymal stem cells from placenta. The method for obtaining the maternal mesenchymal stem cells from the placenta comprises the following steps: obtaining placenta tissues from a position, which are within 5cm away from the radius of an umbilical cord and are 0.5 cm away from a fetal surface, of the placenta; and carrying out enzyme digestion treatment on placenta tissues by adopting an enzyme digestion solution, and then carrying out cell culture on a product of the enzyme digestion treatment to obtain the maternal mesenchymal stem cells. According to the method disclosed by the invention, the technical problem of separating the maternal mesenchymal stem cells from the placenta is solved, the prepared maternal mesenchymal stem cells are large in quantity and high in purity, neonatal cells are not easy to mix, and the clinical application requirements can be met; the method provided by the invention solves the problems that thequantity of the maternal mesenchymal stem cells obtained from the decidua basalis is small and the decidua basalis is easy to lose, and provides a new scheme and way for obtaining the maternal mesenchymal stem cells from the placenta.

Description

technical field [0001] The present application relates to the technical field of mesenchymal stem cell preparation, in particular to a method for obtaining maternally derived mesenchymal stem cells from placenta. Background technique [0002] Mesenchymal stem cells (MSCs) are a group of pluripotent stem cells derived from mesoderm with self-renewal and multilineage differentiation potential. It was first discovered from mouse hematopoietic organs by Friedenstein et al., and proved that it can differentiate into osteoblasts and adipocytes in vitro. Studies have shown that mesenchymal stem cells are widely present in bone marrow, umbilical cord placenta, fat and other tissues. [0003] The placenta is an important organ for material exchange between the fetus and the mother. It is a tissue-combining organ between the mother and the child formed by the joint growth of the embryonic embryonic membrane and the maternal endometrium during human pregnancy. The placenta is rich in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2509/00
Inventor 万桦李国喜于航海
Owner 深圳华大基因细胞科技有限责任公司
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